Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis
Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies. In this study we evaluated the asymptomatic malaria status in Nangarhar Province, Afghanistan in 2017. Methods: Overall, 296 finger blood s...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2020-06-01
|
Series: | Iranian Journal of Parasitology |
Subjects: | |
Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2938 |
id |
doaj-16f2bb2feb49424eaa22e4366289412d |
---|---|
record_format |
Article |
spelling |
doaj-16f2bb2feb49424eaa22e4366289412d2021-04-02T15:19:58ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2020-06-0115210.18502/ijpa.v15i2.32992938Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting AnalysisSayed Hussain MOSAWI0Abdolhossein DALIMI1Najibullah SAFI2Fatemeh GHAFFARIFAR3Javid SADRAEI4Department of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranWorld Health Organization Country Office, Kabul, AfghanistanDepartment of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies. In this study we evaluated the asymptomatic malaria status in Nangarhar Province, Afghanistan in 2017. Methods: Overall, 296 finger blood samples were taken on DNA Banking Cards and microscopic slides from asymptomatic individuals in Jalalabad city. We used a novel post real time PCR high resolution melting analysis beside microscopy and semi-nested multiplex PCR to evaluate status of asymptomatic malaria in this city. Results: The prevalence of asymptomatic malaria in Jalalabad city was determined 1.7% (5/296), 7.43% (22/296) and 7.78% (26/296) by microscopy, Seminested multiplex PCR and qRT-PCR-HRM, respectively. Out of 26 positive cases were detected by qRT-PCR-HRM, 21, 1 and 4 cases were detected P. falciparum, P. vivax and mixed infection of P. falciparum and P. vivax, respectively. Conclusion: Our data indicating on existence of significant number of asymptomatic reservoirs that assists in prolonged endemicity of the disease. On the other hand, the molecular methods are better alternatives for microscopy especially for monitoring of asymptomatic cases of malaria. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2938Asymptomatic malariaAfghanistanHigh resolution melting analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sayed Hussain MOSAWI Abdolhossein DALIMI Najibullah SAFI Fatemeh GHAFFARIFAR Javid SADRAEI |
spellingShingle |
Sayed Hussain MOSAWI Abdolhossein DALIMI Najibullah SAFI Fatemeh GHAFFARIFAR Javid SADRAEI Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis Iranian Journal of Parasitology Asymptomatic malaria Afghanistan High resolution melting analysis |
author_facet |
Sayed Hussain MOSAWI Abdolhossein DALIMI Najibullah SAFI Fatemeh GHAFFARIFAR Javid SADRAEI |
author_sort |
Sayed Hussain MOSAWI |
title |
Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis |
title_short |
Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis |
title_full |
Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis |
title_fullStr |
Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis |
title_full_unstemmed |
Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis |
title_sort |
evaluation of asymptomatic malaria status in eastern of afghanistan using high resolution melting analysis |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2020-06-01 |
description |
Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies. In this study we evaluated the asymptomatic malaria status in Nangarhar Province, Afghanistan in 2017.
Methods: Overall, 296 finger blood samples were taken on DNA Banking Cards and microscopic slides from asymptomatic individuals in Jalalabad city. We used a novel post real time PCR high resolution melting analysis beside microscopy and semi-nested multiplex PCR to evaluate status of asymptomatic malaria in this city.
Results: The prevalence of asymptomatic malaria in Jalalabad city was determined 1.7% (5/296), 7.43% (22/296) and 7.78% (26/296) by microscopy, Seminested multiplex PCR and qRT-PCR-HRM, respectively. Out of 26 positive cases were detected by qRT-PCR-HRM, 21, 1 and 4 cases were detected P. falciparum, P. vivax and mixed infection of P. falciparum and P. vivax, respectively.
Conclusion: Our data indicating on existence of significant number of asymptomatic reservoirs that assists in prolonged endemicity of the disease. On the other hand, the molecular methods are better alternatives for microscopy especially for monitoring of asymptomatic cases of malaria.
|
topic |
Asymptomatic malaria Afghanistan High resolution melting analysis |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2938 |
work_keys_str_mv |
AT sayedhussainmosawi evaluationofasymptomaticmalariastatusineasternofafghanistanusinghighresolutionmeltinganalysis AT abdolhosseindalimi evaluationofasymptomaticmalariastatusineasternofafghanistanusinghighresolutionmeltinganalysis AT najibullahsafi evaluationofasymptomaticmalariastatusineasternofafghanistanusinghighresolutionmeltinganalysis AT fatemehghaffarifar evaluationofasymptomaticmalariastatusineasternofafghanistanusinghighresolutionmeltinganalysis AT javidsadraei evaluationofasymptomaticmalariastatusineasternofafghanistanusinghighresolutionmeltinganalysis |
_version_ |
1721560186694402048 |