Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging
The balance of lung extravascular water depends upon the control of blood flow in the alveolar distribution vessels that feed downstream two districts placed in parallel, the corner vessels and the alveolar septal network. The occurrence of an edemagenic condition appears critical as an increase in...
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doaj-170f23fa71514a23bb2326903e6474db2020-11-25T02:46:21ZengMDPI AGJournal of Imaging2313-433X2019-01-01522210.3390/jimaging5020022jimaging5020022Understanding Vasomotion of Lung Microcirculation by In Vivo ImagingEnrico Mazzuca0Andrea Aliverti1Giuseppe Miserocchi2Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133 Milan, ItalyDepartment of Electronics, Information and Bioengineering, Politecnico di Milano, 20133 Milan, ItalyDepartment of Experimental Medicine, Università di Milano Bicocca, 20900 Milan, ItalyThe balance of lung extravascular water depends upon the control of blood flow in the alveolar distribution vessels that feed downstream two districts placed in parallel, the corner vessels and the alveolar septal network. The occurrence of an edemagenic condition appears critical as an increase in extravascular water endangers the thinness of the air⁻blood barrier, thus negatively affecting the diffusive capacity of the lung. We exposed anesthetized rabbits to an edemagenic factor (12% hypoxia) for 120 min and followed by in vivo imaging the micro-vascular morphology through a “pleural window„ using a stereo microscope at a magnification of 15× (resolution of 7.2 μm). We measured the change in diameter of distribution vessels (50⁻200 μm) and corner vessels (<50 μm). On average, hypoxia caused a significant decrease in diameter of both smaller distribution vessels (about ~50%) and corner vessels (about ~25%) at 30 min. After 120 min, reperfusion occurred. Regional differences in perivascular interstitial volume were observed and could be correlated with differences in blood flow control. To understand such difference, we modelled imaged alveolar capillary units, obtained by Voronoi method, integrating microvascular pressure parameters with capillary filtration. Results of the analysis suggested that at 120 min, alveolar blood flow was diverted to the corner vessels in larger alveoli, which were found also to undergo a greater filtration indicating greater proneness to develop lung edema.https://www.mdpi.com/2313-433X/5/2/22in vivo microscopylung capillariesedemaimage-based modeling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Enrico Mazzuca Andrea Aliverti Giuseppe Miserocchi |
spellingShingle |
Enrico Mazzuca Andrea Aliverti Giuseppe Miserocchi Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging Journal of Imaging in vivo microscopy lung capillaries edema image-based modeling |
author_facet |
Enrico Mazzuca Andrea Aliverti Giuseppe Miserocchi |
author_sort |
Enrico Mazzuca |
title |
Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging |
title_short |
Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging |
title_full |
Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging |
title_fullStr |
Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging |
title_full_unstemmed |
Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging |
title_sort |
understanding vasomotion of lung microcirculation by in vivo imaging |
publisher |
MDPI AG |
series |
Journal of Imaging |
issn |
2313-433X |
publishDate |
2019-01-01 |
description |
The balance of lung extravascular water depends upon the control of blood flow in the alveolar distribution vessels that feed downstream two districts placed in parallel, the corner vessels and the alveolar septal network. The occurrence of an edemagenic condition appears critical as an increase in extravascular water endangers the thinness of the air⁻blood barrier, thus negatively affecting the diffusive capacity of the lung. We exposed anesthetized rabbits to an edemagenic factor (12% hypoxia) for 120 min and followed by in vivo imaging the micro-vascular morphology through a “pleural window„ using a stereo microscope at a magnification of 15× (resolution of 7.2 μm). We measured the change in diameter of distribution vessels (50⁻200 μm) and corner vessels (<50 μm). On average, hypoxia caused a significant decrease in diameter of both smaller distribution vessels (about ~50%) and corner vessels (about ~25%) at 30 min. After 120 min, reperfusion occurred. Regional differences in perivascular interstitial volume were observed and could be correlated with differences in blood flow control. To understand such difference, we modelled imaged alveolar capillary units, obtained by Voronoi method, integrating microvascular pressure parameters with capillary filtration. Results of the analysis suggested that at 120 min, alveolar blood flow was diverted to the corner vessels in larger alveoli, which were found also to undergo a greater filtration indicating greater proneness to develop lung edema. |
topic |
in vivo microscopy lung capillaries edema image-based modeling |
url |
https://www.mdpi.com/2313-433X/5/2/22 |
work_keys_str_mv |
AT enricomazzuca understandingvasomotionoflungmicrocirculationbyinvivoimaging AT andreaaliverti understandingvasomotionoflungmicrocirculationbyinvivoimaging AT giuseppemiserocchi understandingvasomotionoflungmicrocirculationbyinvivoimaging |
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