Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages
The fruit and root of Clausena lansium are remedy for bronchitis in oriental traditional medicine. Extracts from Clausena lansium are reported to have antioxidant, anti-inflammatory, and anticancer activities. This study is designed to investigate whether the ethanol extract of Clausena lansium (Lou...
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Series: | European Journal of Inflammation |
Online Access: | https://doi.org/10.1177/2058739219841973 |
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doaj-175b312d09d249488f9f2fd2bce38a9b2020-11-25T03:48:01ZengSAGE PublishingEuropean Journal of Inflammation2058-73922019-04-011710.1177/2058739219841973Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophagesGuihong Huang0Juan Li1Wanlian Li2Tianxu Liu3Guojun Jiang4Ting Wu5Yong Zou6Junhe Huang7Liqun Tao8Zhaoming Zhu9Jiakui Yue10Xiaoling Zhang11Shengjun Xiao12Department of Pharmacy, The second Affiliated Hospital of Guilin Medical University, Guilin, P.R. ChinaLingui Clinical College, Guilin Medical University, Guilin, P.R. ChinaGuangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Guilin, P.R. ChinaLingui Clinical College, Guilin Medical University, Guilin, P.R. ChinaLingui Clinical College, Guilin Medical University, Guilin, P.R. ChinaLingui Clinical College, Guilin Medical University, Guilin, P.R. ChinaDepartment of Pharmacy, The second Affiliated Hospital of Guilin Medical University, Guilin, P.R. ChinaDepartment of Pharmacy, The second Affiliated Hospital of Guilin Medical University, Guilin, P.R. ChinaDepartment of Pharmacy, The second Affiliated Hospital of Guilin Medical University, Guilin, P.R. ChinaDepartment of Pharmacy, The second Affiliated Hospital of Guilin Medical University, Guilin, P.R. ChinaDepartment of Pharmacy, The second Affiliated Hospital of Guilin Medical University, Guilin, P.R. ChinaDepartment of Physiology, Faculty of Basic Medical Sciences, Guilin Medical University, Guilin, P.R. ChinaDepartment of Pathology, The second Affiliated Hospital of Guilin Medical University, Guilin, P.R. ChinaThe fruit and root of Clausena lansium are remedy for bronchitis in oriental traditional medicine. Extracts from Clausena lansium are reported to have antioxidant, anti-inflammatory, and anticancer activities. This study is designed to investigate whether the ethanol extract of Clausena lansium (Lour.) Skeels could inhibit the lipopolysaccharides (LPS)-induced inflammatory in RAW 264.7 macrophages and the underlining mechanisms. Enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) are used to detect the secretion of tumor necrosis factor alpha (TNF-α) protein and expression of TNF-α mRNA, respectively. RT-PCR and Western blotting are used to determine the mRNA and protein levels of TLR4, MYD88, and TRAF6, respectively. The extract of Clausena lansium suppressed the expression and secretion of TNF-α, which is released by RAW 264.7 cells after LPS induction. Meanwhile, the expression of TLR4, MYD88, and TRAF6 are decreased by extracts from Clausena lansium . Meanwhile, NBP2-29328, an inhibitor of MYD88, synergistically enhances the inhibiting effect of extract from Clausena lansium . The results imply that ethanol extract from Clausena lansium attenuate macrophage-mediated inflammation and TLR4/MYD88/TRAF6 pathway is its potential target.https://doi.org/10.1177/2058739219841973 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guihong Huang Juan Li Wanlian Li Tianxu Liu Guojun Jiang Ting Wu Yong Zou Junhe Huang Liqun Tao Zhaoming Zhu Jiakui Yue Xiaoling Zhang Shengjun Xiao |
spellingShingle |
Guihong Huang Juan Li Wanlian Li Tianxu Liu Guojun Jiang Ting Wu Yong Zou Junhe Huang Liqun Tao Zhaoming Zhu Jiakui Yue Xiaoling Zhang Shengjun Xiao Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages European Journal of Inflammation |
author_facet |
Guihong Huang Juan Li Wanlian Li Tianxu Liu Guojun Jiang Ting Wu Yong Zou Junhe Huang Liqun Tao Zhaoming Zhu Jiakui Yue Xiaoling Zhang Shengjun Xiao |
author_sort |
Guihong Huang |
title |
Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages |
title_short |
Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages |
title_full |
Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages |
title_fullStr |
Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages |
title_full_unstemmed |
Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages |
title_sort |
suppression of inflammation by ethanol extract of clausena lansium via modulation of tlr4/myd88/traf6 signaling pathway in raw 264.7 macrophages |
publisher |
SAGE Publishing |
series |
European Journal of Inflammation |
issn |
2058-7392 |
publishDate |
2019-04-01 |
description |
The fruit and root of Clausena lansium are remedy for bronchitis in oriental traditional medicine. Extracts from Clausena lansium are reported to have antioxidant, anti-inflammatory, and anticancer activities. This study is designed to investigate whether the ethanol extract of Clausena lansium (Lour.) Skeels could inhibit the lipopolysaccharides (LPS)-induced inflammatory in RAW 264.7 macrophages and the underlining mechanisms. Enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) are used to detect the secretion of tumor necrosis factor alpha (TNF-α) protein and expression of TNF-α mRNA, respectively. RT-PCR and Western blotting are used to determine the mRNA and protein levels of TLR4, MYD88, and TRAF6, respectively. The extract of Clausena lansium suppressed the expression and secretion of TNF-α, which is released by RAW 264.7 cells after LPS induction. Meanwhile, the expression of TLR4, MYD88, and TRAF6 are decreased by extracts from Clausena lansium . Meanwhile, NBP2-29328, an inhibitor of MYD88, synergistically enhances the inhibiting effect of extract from Clausena lansium . The results imply that ethanol extract from Clausena lansium attenuate macrophage-mediated inflammation and TLR4/MYD88/TRAF6 pathway is its potential target. |
url |
https://doi.org/10.1177/2058739219841973 |
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