The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian population
Aims: Lung cancer is still the leading cause of cancer mortality in all over the world. Nicotine and its derivatives are the most well-known carcinogens that participate in both etiology and progression of lung cancer. The objective of the current study was to investigate whether single nucleotide p...
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doaj-1770434e1cae486da2ed1d26aa46785f2021-10-04T10:51:17ZengElsevierHeliyon2405-84402021-09-0179e07867The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian populationParisa Veiskarami0Massoud Houshmand1Sharareh Seifi2Nafiseh Ansarinejad3Farshid Fardad4Bahareh Abbasi5Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranDepartment of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran; Research Center, Knowledge University, Erbil, Kurdistan Region, IraqChronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Hematology and Medical Oncology, Rasool Akram Hospital, Iran University of Medical Sciences, Tehran, IranDepartment of Hematology and Medical Oncology, Rasool Akram Hospital, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran; Corresponding author.Aims: Lung cancer is still the leading cause of cancer mortality in all over the world. Nicotine and its derivatives are the most well-known carcinogens that participate in both etiology and progression of lung cancer. The objective of the current study was to investigate whether single nucleotide polymorphisms (SNPs) rs1051730C > T in CHRNA3 and rs3842A > G in ABCB1, two genes contributing in the mechanism of disposition and metabolism of nicotine and its derivatives, could modify the risk of developing lung cancer, as well as nicotine dependence in Iranian. Main methods: The genotyping analysis for these two SNPs was conducted in a case-control study of 108 lung cancer cases and 120 healthy controls using ARMS-PCR and Tetra-primer ARMS-PCR techniques. The correlation between studied SNPs and lung cancer was assessed by the regression analysis. Key findings: We observed a significant association between lung cancer and rs1051730C > T by using four genetic models: allele (OR:1.83; 95% CI:1.24–2.6; p = 0.002), dominant (OR: 2.19; 95% CI:1.27–3.78; p = 0.005), recessive (OR: 2.25; 95% CI: 1.02–4.95; p = 0.043) and additive (TT vs CC: OR:3.25; 95% CI:1.38–7.60; p = 0.007, CT vs CC: OR:1.96; 95% CI:1.10–3.48; p = 0.021). Furthermore, a significant association between this variant and nicotine dependence (OR: 2.27; 95% CI: 1.52–3.39; p = 0.00005) was reported. However, no association was found for rs3842A > G. Significance: The results suggested that the CHRNA3 rs1051730C > T via a smoking-dependent manner could modify susceptibility to lung cancer among Iranian population.http://www.sciencedirect.com/science/article/pii/S2405844021019708Lung cancerNicotine dependencePolymorphismCHRNA3 rs1051730 C>TABCB1 rs3842 A>G |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Parisa Veiskarami Massoud Houshmand Sharareh Seifi Nafiseh Ansarinejad Farshid Fardad Bahareh Abbasi |
spellingShingle |
Parisa Veiskarami Massoud Houshmand Sharareh Seifi Nafiseh Ansarinejad Farshid Fardad Bahareh Abbasi The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian population Heliyon Lung cancer Nicotine dependence Polymorphism CHRNA3 rs1051730 C>T ABCB1 rs3842 A>G |
author_facet |
Parisa Veiskarami Massoud Houshmand Sharareh Seifi Nafiseh Ansarinejad Farshid Fardad Bahareh Abbasi |
author_sort |
Parisa Veiskarami |
title |
The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian population |
title_short |
The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian population |
title_full |
The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian population |
title_fullStr |
The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian population |
title_full_unstemmed |
The effect of CHRNA3 rs1051730 C>T and ABCB1 rs3842 A>G polymorphisms on non-small cell lung cancer and nicotine dependence in Iranian population |
title_sort |
effect of chrna3 rs1051730 c>t and abcb1 rs3842 a>g polymorphisms on non-small cell lung cancer and nicotine dependence in iranian population |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2021-09-01 |
description |
Aims: Lung cancer is still the leading cause of cancer mortality in all over the world. Nicotine and its derivatives are the most well-known carcinogens that participate in both etiology and progression of lung cancer. The objective of the current study was to investigate whether single nucleotide polymorphisms (SNPs) rs1051730C > T in CHRNA3 and rs3842A > G in ABCB1, two genes contributing in the mechanism of disposition and metabolism of nicotine and its derivatives, could modify the risk of developing lung cancer, as well as nicotine dependence in Iranian. Main methods: The genotyping analysis for these two SNPs was conducted in a case-control study of 108 lung cancer cases and 120 healthy controls using ARMS-PCR and Tetra-primer ARMS-PCR techniques. The correlation between studied SNPs and lung cancer was assessed by the regression analysis. Key findings: We observed a significant association between lung cancer and rs1051730C > T by using four genetic models: allele (OR:1.83; 95% CI:1.24–2.6; p = 0.002), dominant (OR: 2.19; 95% CI:1.27–3.78; p = 0.005), recessive (OR: 2.25; 95% CI: 1.02–4.95; p = 0.043) and additive (TT vs CC: OR:3.25; 95% CI:1.38–7.60; p = 0.007, CT vs CC: OR:1.96; 95% CI:1.10–3.48; p = 0.021). Furthermore, a significant association between this variant and nicotine dependence (OR: 2.27; 95% CI: 1.52–3.39; p = 0.00005) was reported. However, no association was found for rs3842A > G. Significance: The results suggested that the CHRNA3 rs1051730C > T via a smoking-dependent manner could modify susceptibility to lung cancer among Iranian population. |
topic |
Lung cancer Nicotine dependence Polymorphism CHRNA3 rs1051730 C>T ABCB1 rs3842 A>G |
url |
http://www.sciencedirect.com/science/article/pii/S2405844021019708 |
work_keys_str_mv |
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