Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection.
Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interpla...
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doaj-177ad084a98c4563a6c3cad10b7b526c2020-11-25T02:04:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2644410.1371/journal.pone.0026444Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection.Nuno CarinhasAaron Mark RobitailleSuzette MoesManuel José Teixeira CarrondoPaul JenoeRui OliveiraPaula Marques AlvesBaculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome quantitation of S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism, endoplasmic reticulum and oxidative stress, yet not investigated in the scope of baculovirus infection. Further, the reduced expression of key viral-encoded proteins early in the infection cycle is suggested to be related with decreased viral replication at high cell density culture. These findings have implications for virological research and improvement of baculovirus-based bioprocesses.http://europepmc.org/articles/PMC3196586?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nuno Carinhas Aaron Mark Robitaille Suzette Moes Manuel José Teixeira Carrondo Paul Jenoe Rui Oliveira Paula Marques Alves |
spellingShingle |
Nuno Carinhas Aaron Mark Robitaille Suzette Moes Manuel José Teixeira Carrondo Paul Jenoe Rui Oliveira Paula Marques Alves Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. PLoS ONE |
author_facet |
Nuno Carinhas Aaron Mark Robitaille Suzette Moes Manuel José Teixeira Carrondo Paul Jenoe Rui Oliveira Paula Marques Alves |
author_sort |
Nuno Carinhas |
title |
Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. |
title_short |
Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. |
title_full |
Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. |
title_fullStr |
Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. |
title_full_unstemmed |
Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. |
title_sort |
quantitative proteomics of spodoptera frugiperda cells during growth and baculovirus infection. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome quantitation of S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism, endoplasmic reticulum and oxidative stress, yet not investigated in the scope of baculovirus infection. Further, the reduced expression of key viral-encoded proteins early in the infection cycle is suggested to be related with decreased viral replication at high cell density culture. These findings have implications for virological research and improvement of baculovirus-based bioprocesses. |
url |
http://europepmc.org/articles/PMC3196586?pdf=render |
work_keys_str_mv |
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