Enhanced Production of Bryonolic Acid in <i>Trichosanthes cucumerina</i> L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities
Bryonolic acid is a triterpenoid compound found in cucurbitaceous roots. Due to its biological activities, this compound gets more attention to improve production. Herein, we carried out efficient ways with high bryonolic acid productions from <i>Trichosanthes cucumerina</i> L., a Thai m...
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doaj-177aea606eff47fba7cbf7ef4ad2ff572020-11-25T03:19:59ZengMDPI AGPlants2223-77472020-06-01970970910.3390/plants9060709Enhanced Production of Bryonolic Acid in <i>Trichosanthes cucumerina</i> L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological ActivitiesPornpatsorn Lertphadungkit0Jiraphong Suksiriworapong1Veena Satitpatipan2Supaart Sirikantaramas3Amaraporn Wongrakpanich4Somnuk Bunsupa5Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandMolecular Crop Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandDepartment of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandBryonolic acid is a triterpenoid compound found in cucurbitaceous roots. Due to its biological activities, this compound gets more attention to improve production. Herein, we carried out efficient ways with high bryonolic acid productions from <i>Trichosanthes cucumerina</i> L., a Thai medicinal plant utilizing plant cell cultures. The results showed that calli (24.65 ± 1.97 mg/g dry weight) and cell suspensions (15.69 ± 0.78 mg/g dry weight) exhibited the highest bryonolic acid productions compared with natural roots (approximately 2 mg/g dry weight). In the presence of three elicitors (methyl jasmonate, yeast extract, and chitosan), cell suspensions treated with 1 mg/mL of chitosan for eight days led to higher bryonolic acid contents (23.56 ± 1.68 mg/g dry weight). Interestingly, cell culture and root extracts with high bryonolic acid contents resulted in significantly higher percent cell viabilities than those observed under control (1%<i> v/v </i>DMSO) treatment in Saos-2 and MCF-7 cells. The present study indicated that <i>T. cucumerina</i> L. cell cultures are alternative and efficient to produce the biologically important secondary metabolite.https://www.mdpi.com/2223-7747/9/6/709bryonolic acidcalluscell suspensionelicitors<i>Trichosanthes cucumerina</i> L. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pornpatsorn Lertphadungkit Jiraphong Suksiriworapong Veena Satitpatipan Supaart Sirikantaramas Amaraporn Wongrakpanich Somnuk Bunsupa |
spellingShingle |
Pornpatsorn Lertphadungkit Jiraphong Suksiriworapong Veena Satitpatipan Supaart Sirikantaramas Amaraporn Wongrakpanich Somnuk Bunsupa Enhanced Production of Bryonolic Acid in <i>Trichosanthes cucumerina</i> L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities Plants bryonolic acid callus cell suspension elicitors <i>Trichosanthes cucumerina</i> L. |
author_facet |
Pornpatsorn Lertphadungkit Jiraphong Suksiriworapong Veena Satitpatipan Supaart Sirikantaramas Amaraporn Wongrakpanich Somnuk Bunsupa |
author_sort |
Pornpatsorn Lertphadungkit |
title |
Enhanced Production of Bryonolic Acid in <i>Trichosanthes cucumerina</i> L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities |
title_short |
Enhanced Production of Bryonolic Acid in <i>Trichosanthes cucumerina</i> L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities |
title_full |
Enhanced Production of Bryonolic Acid in <i>Trichosanthes cucumerina</i> L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities |
title_fullStr |
Enhanced Production of Bryonolic Acid in <i>Trichosanthes cucumerina</i> L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities |
title_full_unstemmed |
Enhanced Production of Bryonolic Acid in <i>Trichosanthes cucumerina</i> L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities |
title_sort |
enhanced production of bryonolic acid in <i>trichosanthes cucumerina</i> l. (thai cultivar) cell cultures by elicitors and their biological activities |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2020-06-01 |
description |
Bryonolic acid is a triterpenoid compound found in cucurbitaceous roots. Due to its biological activities, this compound gets more attention to improve production. Herein, we carried out efficient ways with high bryonolic acid productions from <i>Trichosanthes cucumerina</i> L., a Thai medicinal plant utilizing plant cell cultures. The results showed that calli (24.65 ± 1.97 mg/g dry weight) and cell suspensions (15.69 ± 0.78 mg/g dry weight) exhibited the highest bryonolic acid productions compared with natural roots (approximately 2 mg/g dry weight). In the presence of three elicitors (methyl jasmonate, yeast extract, and chitosan), cell suspensions treated with 1 mg/mL of chitosan for eight days led to higher bryonolic acid contents (23.56 ± 1.68 mg/g dry weight). Interestingly, cell culture and root extracts with high bryonolic acid contents resulted in significantly higher percent cell viabilities than those observed under control (1%<i> v/v </i>DMSO) treatment in Saos-2 and MCF-7 cells. The present study indicated that <i>T. cucumerina</i> L. cell cultures are alternative and efficient to produce the biologically important secondary metabolite. |
topic |
bryonolic acid callus cell suspension elicitors <i>Trichosanthes cucumerina</i> L. |
url |
https://www.mdpi.com/2223-7747/9/6/709 |
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