Summary: | To produce high-quality broccoli microgreens, suitable light intensity for growth and phytochemical contents of broccoli microgreens in an artificial light plant factory were studied. Broccoli microgreens were irradiated under different photosynthetic photon flux density (PPFD): 30, 50, 70 and 90 μmol·m<sup>−2</sup>·s<sup>−1</sup> with red: green: blue = 1:1:1 light-emitting diodes (LEDs). The broccoli microgreens grown under 50 μmol·m<sup>−2</sup>·s<sup>−1</sup> had the highest fresh weight, dry weight, and moisture content, while the phytochemical contents were the lowest. With increasing light intensity, the chlorophyll content increased, whereas the carotenoid content decreased. The contents of soluble protein, soluble sugar, free amino acid, flavonoid, vitamin C, and glucosinolates except for progoitrin in broccoli microgreens were higher under 70 μmol·m<sup>−2</sup>·s<sup>−1</sup>. Overall, 50 μmol·m<sup>−2</sup>·s<sup>−1</sup> was the optimal light intensity for enhancement of growth of broccoli microgreens, while 70 μmol·m<sup>−2</sup>·s<sup>−1</sup> was more feasible for improving the phytochemicals of broccoli microgreens in an artificial light plant factory.
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