TRANSFER GEN BADH2 TERMUTASI VARIETAS AROMATIK MENTIK WANGI KE VARIETAS NONAROMATIK CIHERANG

• Main Authors: Djarot Sasongko Hami Seno, Bambang Padmadi, Dewi Praptiwi, Sugihartati, Taufiq, Muhammad Taufan Fatahajudin, Helmy Ramadhan Al Anshary, Tri Joko Santoso, Zainal Alim Mas'ud
• Format: Article
• Language: English
• Published: Bogor Agricultural University 2011-04-01
• Series: Jurnal Ilmu Pertanian Indonesia
• Subjects: Backcross; Bradbury; fragrant; Mentik Wangi; badh2; site-directed crossing
• Online Access: http://journal.ipb.ac.id/index.php/JIPI/article/view/6593

Replacement of non-fragrant-native badh2 gene with mutated badh2 of fragrant rice is an alternative to engineer new fragrant rice varieties with good agronomic traits as those of non-fragrant. Fragrant gene (mutated badh2) of Mentik wangi donor was introgressed into non-fragrant Ciherang host through site-directed crossing. Mentik Wangi was crossed with Ciherang, and the progeny was further backcross until BC3F1. Bradbury marka-assisted PCR was used to select progeny in every cross and backcross generation. Ciherang, Mentik Wangi, and their cross/backcross showed different PCR profiles. The statues of badh2 gene (native/mutated), as well as alleles (homozygote/heterozygote) between samples were identified. Mutated badh2-introgression was also observed within the selected heterozygote cross or backcross progenies (F1, BC1, BC2, and BC3), indicated successful transfer of mutated badh2 gene from donor to host