9-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression
Objective: To investigate the potential anti-aging mechanism of 9-hydroxy-6,7-dimethoxydalbergiquinol (HDDQ) on hydrogen peroxide (H2O2)-induced oxidative stress in human dermal fibroblasts (HDFs). Methods: The effect of HDDQ on cell viability was assessed by MTT assay, and the effects of HDDQ on se...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2021-01-01
|
Series: | Asian Pacific Journal of Tropical Biomedicine |
Subjects: | |
Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=89;epage=96;aulast=Lim |
id |
doaj-17c8e57d04584728b314bcc13b059bc0 |
---|---|
record_format |
Article |
spelling |
doaj-17c8e57d04584728b314bcc13b059bc02021-02-03T05:47:58ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222021-01-01112899610.4103/2221-1691.3010589-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expressionSeok-Hee LimBing Si LiRi Zhe ZhuByung-Min ChoiObjective: To investigate the potential anti-aging mechanism of 9-hydroxy-6,7-dimethoxydalbergiquinol (HDDQ) on hydrogen peroxide (H2O2)-induced oxidative stress in human dermal fibroblasts (HDFs). Methods: The effect of HDDQ on cell viability was assessed by MTT assay, and the effects of HDDQ on senescence-like phenotypes were determined by senescence-associated β-galactosidase (SA-β-gal) staining, Western blotting analysis, and a cell proliferation assay. The expression level and activity of sirtuin-1 (SIRT1) induced by HDDQ were also measured. Results: HDDQ reversed senescence-like phenotypes in the oxidant-challenged model, through reducing SA-β-gal activity and promoting cell growth. Meanwhile, decreases in ac-p53, p21Cip1/WAF1, and p16Ink4a and an increase in pRb were observed. HDDQ induced the expression of SIRT1 in a concentration- and time-dependent manner. Moreover, HDDQ inhibited H2O2-induced phosphorylation of Akt by SIRT1 up-regulation and reduced SA-β-gal staining. Conclusions: HDDQ inhibits H2O2-induced premature senescence and upregulation of SIRT1 expression plays a vital role in the inhibition of the senescence phenotype in HDFs.http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=89;epage=96;aulast=Lim9-hydroxy-67-dimethoxydalbergiquinol; hydrogen peroxide; senescence; sirtuin-1; human dermal fibroblasts |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seok-Hee Lim Bing Si Li Ri Zhe Zhu Byung-Min Choi |
spellingShingle |
Seok-Hee Lim Bing Si Li Ri Zhe Zhu Byung-Min Choi 9-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression Asian Pacific Journal of Tropical Biomedicine 9-hydroxy-6 7-dimethoxydalbergiquinol; hydrogen peroxide; senescence; sirtuin-1; human dermal fibroblasts |
author_facet |
Seok-Hee Lim Bing Si Li Ri Zhe Zhu Byung-Min Choi |
author_sort |
Seok-Hee Lim |
title |
9-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression |
title_short |
9-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression |
title_full |
9-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression |
title_fullStr |
9-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression |
title_full_unstemmed |
9-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression |
title_sort |
9-hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Pacific Journal of Tropical Biomedicine |
issn |
2221-1691 2588-9222 |
publishDate |
2021-01-01 |
description |
Objective: To investigate the potential anti-aging mechanism of 9-hydroxy-6,7-dimethoxydalbergiquinol (HDDQ) on hydrogen peroxide (H2O2)-induced oxidative stress in human dermal fibroblasts (HDFs).
Methods: The effect of HDDQ on cell viability was assessed by MTT assay, and the effects of HDDQ on senescence-like phenotypes were determined by senescence-associated β-galactosidase (SA-β-gal) staining, Western blotting analysis, and a cell proliferation assay. The expression level and activity of sirtuin-1 (SIRT1) induced by HDDQ were also measured.
Results: HDDQ reversed senescence-like phenotypes in the oxidant-challenged model, through reducing SA-β-gal activity and promoting cell growth. Meanwhile, decreases in ac-p53, p21Cip1/WAF1, and p16Ink4a and an increase in pRb were observed. HDDQ induced the expression of SIRT1 in a concentration- and time-dependent manner. Moreover, HDDQ inhibited H2O2-induced phosphorylation of Akt by SIRT1 up-regulation and reduced SA-β-gal staining.
Conclusions: HDDQ inhibits H2O2-induced premature senescence and upregulation of SIRT1 expression plays a vital role in the inhibition of the senescence phenotype in HDFs. |
topic |
9-hydroxy-6 7-dimethoxydalbergiquinol; hydrogen peroxide; senescence; sirtuin-1; human dermal fibroblasts |
url |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=89;epage=96;aulast=Lim |
work_keys_str_mv |
AT seokheelim 9hydroxy67dimethoxydalbergiquinolsuppresseshydrogenperoxideinducedsenescenceinhumandermalfibroblaststhroughinductionofsirtuin1expression AT bingsili 9hydroxy67dimethoxydalbergiquinolsuppresseshydrogenperoxideinducedsenescenceinhumandermalfibroblaststhroughinductionofsirtuin1expression AT rizhezhu 9hydroxy67dimethoxydalbergiquinolsuppresseshydrogenperoxideinducedsenescenceinhumandermalfibroblaststhroughinductionofsirtuin1expression AT byungminchoi 9hydroxy67dimethoxydalbergiquinolsuppresseshydrogenperoxideinducedsenescenceinhumandermalfibroblaststhroughinductionofsirtuin1expression |
_version_ |
1724289032628731904 |