A Styrene-alt-Maleic Acid Copolymer Is an Effective Inhibitor of R5 and X4 Human Immunodeficiency Virus Type 1 Infection
An alternating copolymer of styrene and maleic acid (alt-PSMA) differs from other polyanionic antiviral agents in that the negative charges of alt-PSMA are provided by carboxylic acid groups instead...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2010-01-01
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| Series: | Journal of Biomedicine and Biotechnology |
| Online Access: | http://dx.doi.org/10.1155/2010/548749 |
| Summary: | An alternating
copolymer of styrene and maleic acid
(alt-PSMA) differs from other
polyanionic antiviral agents in that the
negative charges of alt-PSMA
are provided by carboxylic acid groups instead
of sulfate or sulfonate moieties. We
hypothesized that alt-PSMA
would have activity against human
immunodeficiency virus type 1 (HIV-1) comparable
to other polyanions, such as the related
compound, poly(sodium 4-styrene sulfonate)
(PSS). In assays using cell lines and primary
immune cells, alt-PSMA was
characterized by low cytotoxicity and effective
inhibition of infection by HIV-1 BaL and IIIB as
well as clinical isolates of subtypes A, B, and
C. In mechanism of action assays, in which each
compound was added to cells and subsequently
removed prior to HIV-1 infection
(“washout” assay),
alt-PSMA caused no enhancement
of infection, while PSS washout increased
infection 70% above control levels. These
studies demonstrate that
alt-PSMA is an effective HIV-1
inhibitor with properties that warrant further
investigation. |
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| ISSN: | 1110-7243 1110-7251 |