A solution to prevent secondary flow in adherent cell cultures
High quality cell cultures require reliable laboratory practices. Today's small-scale in vitro cell culture format is dominated by circular topology vessels, with the inherent disadvantage of secondary flow induced each time the cell cultures are repositioned. The secondary flow generates uneve...
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The Company of Biologists
2019-07-01
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doaj-1800bc4a1aea49abb48ddbd1082c5b892021-06-02T14:58:04ZengThe Company of BiologistsBiology Open2046-63902019-07-018710.1242/bio.045294045294A solution to prevent secondary flow in adherent cell culturesPeter Szaraz0Matthew Librach1Poonam Mander2Banafshe Hoseini3Max Librach4Farwah Iqbal5Clifford Librach6 Create Program Inc., Suite 412, Toronto, Ontario M5G 1N8, Canada Create Program Inc., Suite 412, Toronto, Ontario M5G 1N8, Canada Create Program Inc., Suite 412, Toronto, Ontario M5G 1N8, Canada Create Program Inc., Suite 412, Toronto, Ontario M5G 1N8, Canada Create Program Inc., Suite 412, Toronto, Ontario M5G 1N8, Canada Dept. Physiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada Create Program Inc., Suite 412, Toronto, Ontario M5G 1N8, Canada High quality cell cultures require reliable laboratory practices. Today's small-scale in vitro cell culture format is dominated by circular topology vessels, with the inherent disadvantage of secondary flow induced each time the cell cultures are repositioned. The secondary flow generates uneven sedimentation and adherence that negatively impacts cell culture quality. Here we show a modification of the circular culture vessel that abrogates these disturbances. Cell culture wells were augmented with a central column to diminish secondary flow. Human carcinoma cell lines (BeWo, JEG-3), mesenchymal stem cells [human umbilical cord perivascular cells (HUCPVC)] and mouse embryonic fibroblasts (MEF) were cultured in both column-augmented and regular culture wells. Human carcinoma cell cultures showed even cell densities and significantly more viable cells in column-augmented vessels. In FTM HUCPVC cultures, cell surface MSC marker (CD90, CD105) expression and cell differentiation-related gene expression patterns were significantly more homogeneous in column-augmented vessels. MEF cells in column-augmented culture vessels showed a more consistent expression of IGF-1. Column-augmented cell culture vessels significantly improve the homogeneity of adherent cell cultures by mitigating the adverse effect of the secondary flow. This article has an associated First Person interview with the first author of the paper.http://bio.biologists.org/content/8/7/bio045294Adherent cell cultureCell differentiationMesenchymal stem cellsFeeder cell culture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peter Szaraz Matthew Librach Poonam Mander Banafshe Hoseini Max Librach Farwah Iqbal Clifford Librach |
spellingShingle |
Peter Szaraz Matthew Librach Poonam Mander Banafshe Hoseini Max Librach Farwah Iqbal Clifford Librach A solution to prevent secondary flow in adherent cell cultures Biology Open Adherent cell culture Cell differentiation Mesenchymal stem cells Feeder cell culture |
author_facet |
Peter Szaraz Matthew Librach Poonam Mander Banafshe Hoseini Max Librach Farwah Iqbal Clifford Librach |
author_sort |
Peter Szaraz |
title |
A solution to prevent secondary flow in adherent cell cultures |
title_short |
A solution to prevent secondary flow in adherent cell cultures |
title_full |
A solution to prevent secondary flow in adherent cell cultures |
title_fullStr |
A solution to prevent secondary flow in adherent cell cultures |
title_full_unstemmed |
A solution to prevent secondary flow in adherent cell cultures |
title_sort |
solution to prevent secondary flow in adherent cell cultures |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2019-07-01 |
description |
High quality cell cultures require reliable laboratory practices. Today's small-scale in vitro cell culture format is dominated by circular topology vessels, with the inherent disadvantage of secondary flow induced each time the cell cultures are repositioned. The secondary flow generates uneven sedimentation and adherence that negatively impacts cell culture quality. Here we show a modification of the circular culture vessel that abrogates these disturbances. Cell culture wells were augmented with a central column to diminish secondary flow. Human carcinoma cell lines (BeWo, JEG-3), mesenchymal stem cells [human umbilical cord perivascular cells (HUCPVC)] and mouse embryonic fibroblasts (MEF) were cultured in both column-augmented and regular culture wells. Human carcinoma cell cultures showed even cell densities and significantly more viable cells in column-augmented vessels. In FTM HUCPVC cultures, cell surface MSC marker (CD90, CD105) expression and cell differentiation-related gene expression patterns were significantly more homogeneous in column-augmented vessels. MEF cells in column-augmented culture vessels showed a more consistent expression of IGF-1. Column-augmented cell culture vessels significantly improve the homogeneity of adherent cell cultures by mitigating the adverse effect of the secondary flow. This article has an associated First Person interview with the first author of the paper. |
topic |
Adherent cell culture Cell differentiation Mesenchymal stem cells Feeder cell culture |
url |
http://bio.biologists.org/content/8/7/bio045294 |
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