Inhibitory effect of TNF-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations.
<h4>Background</h4>The liver stages of malaria parasites are inhibited by cytokines such as interferon-γ or Interleukin (IL)-6. Binding of these cytokines to their receptors at the surface of the infected hepatocytes leads to the production of nitric oxide (NO) and radical oxygen interme...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011-03-01
|
Series: | PLoS ONE |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21394207/pdf/?tool=EBI |
id |
doaj-18039d74296d45ac84cfab934fef6a55 |
---|---|
record_format |
Article |
spelling |
doaj-18039d74296d45ac84cfab934fef6a552021-03-04T02:02:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-03-0163e1746410.1371/journal.pone.0017464Inhibitory effect of TNF-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations.Nadya DepinayJean Francois FranetichAnne Charlotte GrünerMarjorie MauduitJean-Marc ChavatteAdrian J F LutyGeert-Jan van GemertRobert W SauerweinJean-Michel SiksikLaurent HannounDominique MazierGeorges SnounouLaurent Rénia<h4>Background</h4>The liver stages of malaria parasites are inhibited by cytokines such as interferon-γ or Interleukin (IL)-6. Binding of these cytokines to their receptors at the surface of the infected hepatocytes leads to the production of nitric oxide (NO) and radical oxygen intermediates (ROI), which kill hepatic parasites. However, conflicting results were obtained with TNF-α possibly because of differences in the models used. We have reassessed the role of TNF-α in the different cellular systems used to study the Plasmodium pre-erythrocytic stages.<h4>Methods and findings</h4>Human or mouse TNF-α were tested against human and rodent malaria parasites grown in vitro in human or rodent primary hepatocytes, or in hepatoma cell lines. Our data demonstrated that TNF-α treatment prevents the development of malaria pre-erythrocytic stages. This inhibitory effect however varies with the infecting parasite species and with the nature and origin of the cytokine and hepatocytes. Inhibition was only observed for all parasite species tested when hepatocytes were pre-incubated 24 or 48 hrs before infection and activity was directed only against early hepatic parasite. We further showed that TNF-α inhibition was mediated by a soluble factor present in the supernatant of TNF-α stimulated hepatocytes but it was not related to NO or ROI. Treatment TNF-α prevents the development of human and rodent malaria pre-erythrocytic stages through the activity of a mediator that remains to be identified.<h4>Conclusions</h4>Treatment TNF-α prevents the development of human and rodent malaria pre-erythrocytic stages through the activity of a mediator that remains to be identified. However, the nature of the cytokine-host cell-parasite combination must be carefully considered for extrapolation to the human infection.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21394207/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nadya Depinay Jean Francois Franetich Anne Charlotte Grüner Marjorie Mauduit Jean-Marc Chavatte Adrian J F Luty Geert-Jan van Gemert Robert W Sauerwein Jean-Michel Siksik Laurent Hannoun Dominique Mazier Georges Snounou Laurent Rénia |
spellingShingle |
Nadya Depinay Jean Francois Franetich Anne Charlotte Grüner Marjorie Mauduit Jean-Marc Chavatte Adrian J F Luty Geert-Jan van Gemert Robert W Sauerwein Jean-Michel Siksik Laurent Hannoun Dominique Mazier Georges Snounou Laurent Rénia Inhibitory effect of TNF-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations. PLoS ONE |
author_facet |
Nadya Depinay Jean Francois Franetich Anne Charlotte Grüner Marjorie Mauduit Jean-Marc Chavatte Adrian J F Luty Geert-Jan van Gemert Robert W Sauerwein Jean-Michel Siksik Laurent Hannoun Dominique Mazier Georges Snounou Laurent Rénia |
author_sort |
Nadya Depinay |
title |
Inhibitory effect of TNF-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations. |
title_short |
Inhibitory effect of TNF-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations. |
title_full |
Inhibitory effect of TNF-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations. |
title_fullStr |
Inhibitory effect of TNF-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations. |
title_full_unstemmed |
Inhibitory effect of TNF-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations. |
title_sort |
inhibitory effect of tnf-α on malaria pre-erythrocytic stage development: influence of host hepatocyte/parasite combinations. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-03-01 |
description |
<h4>Background</h4>The liver stages of malaria parasites are inhibited by cytokines such as interferon-γ or Interleukin (IL)-6. Binding of these cytokines to their receptors at the surface of the infected hepatocytes leads to the production of nitric oxide (NO) and radical oxygen intermediates (ROI), which kill hepatic parasites. However, conflicting results were obtained with TNF-α possibly because of differences in the models used. We have reassessed the role of TNF-α in the different cellular systems used to study the Plasmodium pre-erythrocytic stages.<h4>Methods and findings</h4>Human or mouse TNF-α were tested against human and rodent malaria parasites grown in vitro in human or rodent primary hepatocytes, or in hepatoma cell lines. Our data demonstrated that TNF-α treatment prevents the development of malaria pre-erythrocytic stages. This inhibitory effect however varies with the infecting parasite species and with the nature and origin of the cytokine and hepatocytes. Inhibition was only observed for all parasite species tested when hepatocytes were pre-incubated 24 or 48 hrs before infection and activity was directed only against early hepatic parasite. We further showed that TNF-α inhibition was mediated by a soluble factor present in the supernatant of TNF-α stimulated hepatocytes but it was not related to NO or ROI. Treatment TNF-α prevents the development of human and rodent malaria pre-erythrocytic stages through the activity of a mediator that remains to be identified.<h4>Conclusions</h4>Treatment TNF-α prevents the development of human and rodent malaria pre-erythrocytic stages through the activity of a mediator that remains to be identified. However, the nature of the cytokine-host cell-parasite combination must be carefully considered for extrapolation to the human infection. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21394207/pdf/?tool=EBI |
work_keys_str_mv |
AT nadyadepinay inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT jeanfrancoisfranetich inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT annecharlottegruner inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT marjoriemauduit inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT jeanmarcchavatte inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT adrianjfluty inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT geertjanvangemert inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT robertwsauerwein inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT jeanmichelsiksik inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT laurenthannoun inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT dominiquemazier inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT georgessnounou inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations AT laurentrenia inhibitoryeffectoftnfaonmalariapreerythrocyticstagedevelopmentinfluenceofhosthepatocyteparasitecombinations |
_version_ |
1714808966044712960 |