Low level of baseline resistance in recently HCV-infected men who have sex with men with high-risk behaviours
Objectives: Presence of baseline hepatitis C virus (HCV) resistance-associated substitutions (RASs) can impair treatment outcome of direct-acting antivirals. We investigated the prevalence of pre-treatment HCV resistance among recently HCV-infected men who have sex with men (MSM) with high risk beha...
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Format: | Article |
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Elsevier
2021-03-01
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Series: | Journal of Global Antimicrobial Resistance |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716521000242 |
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doaj-1825667bb7214d4aaceb397a01b4711a |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thuy Nguyen Marc-Antoine Valantin Constance Delaugerre Corinne Amiel Emmanuelle Netzer Thomas L’Yavanc Michel Ohayon Nadia Valin Nesrine Day Georges Kreplak Gilles Pialoux Vincent Calvez Jean-Michel Molina Anne-Geneviève Marcelin Eve Todesco |
spellingShingle |
Thuy Nguyen Marc-Antoine Valantin Constance Delaugerre Corinne Amiel Emmanuelle Netzer Thomas L’Yavanc Michel Ohayon Nadia Valin Nesrine Day Georges Kreplak Gilles Pialoux Vincent Calvez Jean-Michel Molina Anne-Geneviève Marcelin Eve Todesco Low level of baseline resistance in recently HCV-infected men who have sex with men with high-risk behaviours Journal of Global Antimicrobial Resistance Men who have sex with men Deep sequencing Hepatitis C virus HCV Resistance transmission Pre-exposure prophylaxis |
author_facet |
Thuy Nguyen Marc-Antoine Valantin Constance Delaugerre Corinne Amiel Emmanuelle Netzer Thomas L’Yavanc Michel Ohayon Nadia Valin Nesrine Day Georges Kreplak Gilles Pialoux Vincent Calvez Jean-Michel Molina Anne-Geneviève Marcelin Eve Todesco |
author_sort |
Thuy Nguyen |
title |
Low level of baseline resistance in recently HCV-infected men who have sex with men with high-risk behaviours |
title_short |
Low level of baseline resistance in recently HCV-infected men who have sex with men with high-risk behaviours |
title_full |
Low level of baseline resistance in recently HCV-infected men who have sex with men with high-risk behaviours |
title_fullStr |
Low level of baseline resistance in recently HCV-infected men who have sex with men with high-risk behaviours |
title_full_unstemmed |
Low level of baseline resistance in recently HCV-infected men who have sex with men with high-risk behaviours |
title_sort |
low level of baseline resistance in recently hcv-infected men who have sex with men with high-risk behaviours |
publisher |
Elsevier |
series |
Journal of Global Antimicrobial Resistance |
issn |
2213-7165 |
publishDate |
2021-03-01 |
description |
Objectives: Presence of baseline hepatitis C virus (HCV) resistance-associated substitutions (RASs) can impair treatment outcome of direct-acting antivirals. We investigated the prevalence of pre-treatment HCV resistance among recently HCV-infected men who have sex with men (MSM) with high risk behaviours, either human immunodeficiency virus (HIV) co-infected or at high risk of HIV acquisition and under pre-exposure prophylaxis (PrEP). Methods: NS5A and NS3 fragments were deep sequenced on pre-treatment samples of 72 subjects using Illumina MiSeq paired-end sequencing technology. Ultra-deep sequencing data were analysed by SmartGene® platform. RASs mentioned in the literature were analysed and interpreted depending on genotype (GT) at 10% cut-off. Results: HCV genotyping showed 36 (50.0%) GT1a, 31 (43.1%) GT4d and 5 (6.9%) GT3a infections. Fifty-five patients (76.4%) were co-infected with HIV and 15 (20.8%) received PrEP. In GT1a viruses, NS3 RASs were found in 4/30 viruses (13.3%; S122 G/N, R155 K and I170 V) and Q80 K polymorphism was present in 14/30 viruses (46.7%). No NS3 RASs were detected in GT4d and GT3a viruses. NS5A RASs were detected in 3/36 GT1a viruses (8.3%; Q30E/R, L31 M and H58 L). NS5A subtype-specific polymorphisms L30R and T58 P were found at high frequencies in 31/31 (100%) and 16/31 (51.6%) GT4d viruses, respectively. One RAS M31 L was also observed along with the polymorphisms L30R and T58 P. No NS5A RASs were detected in GT3a viruses. Conclusion: A low level of RASs to NS3 and NS5A inhibitors in pre-treatment samples was detected in the study population. Our findings reassure the clinical management of HCV infection in this high-risk population. |
topic |
Men who have sex with men Deep sequencing Hepatitis C virus HCV Resistance transmission Pre-exposure prophylaxis |
url |
http://www.sciencedirect.com/science/article/pii/S2213716521000242 |
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doaj-1825667bb7214d4aaceb397a01b4711a2021-06-09T05:58:19ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-03-0124311315Low level of baseline resistance in recently HCV-infected men who have sex with men with high-risk behavioursThuy Nguyen0Marc-Antoine Valantin1Constance Delaugerre2Corinne Amiel3Emmanuelle Netzer4Thomas L’Yavanc5Michel Ohayon6Nadia Valin7Nesrine Day8Georges Kreplak9Gilles Pialoux10Vincent Calvez11Jean-Michel Molina12Anne-Geneviève Marcelin13Eve Todesco14Sorbonne Université, INSERM, Institut Pierre Louis d’Epidémiologie et de Santé Publique (iPLESP), AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de virologie, F-75013 Paris, FranceSorbonne Université, INSERM, Institut Pierre Louis d’Epidémiologie et de Santé Publique (iPLESP), AP-HP, Hôpital Pitié-Salpêtrière, Services de maladies infectieuses et tropicales, F-75013 Paris, FranceAP-HP, Hôpital Saint-Louis, Laboratoire de virologie, Paris, France; INSERM UMR 941, Université de Paris Diderot, Paris, FranceSorbonne Université, Centre d’Immunologie et de Maladies Infectieuses (CIMI) UMRS CR7, Persistent Viral Infection (PVI) Team, Inserm U1135, AP-HP, Groupe Hospitalier Paris Est, Hôpital Tenon, Laboratoire de virologie, F-75020 Paris, FranceINSERM SC10, Villejuif, FranceCentre de santé sexuelle Le 190, Paris, France; Sorbonne Université, AP-HP, Hôpital Tenon, Department of Infectious Diseases, Paris, FranceCentre de santé sexuelle Le 190, Paris, France; Sorbonne Université, AP-HP, Hôpital Tenon, Department of Infectious Diseases, Paris, FranceSorbonne Université, INSERM, Institut Pierre Louis d’Epidémiologie et de Santé Publique (iPLESP), AP-HP, Hôpital Saint Antoine, Department of Infectious Diseases, F-75012 Paris, FranceCerballiance Laboratory, Paris, FranceCerballiance Laboratory, Paris, FranceSorbonne Université, AP-HP, Hôpital Tenon, Department of Infectious Diseases, Paris, FranceSorbonne Université, INSERM, Institut Pierre Louis d’Epidémiologie et de Santé Publique (iPLESP), AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de virologie, F-75013 Paris, FranceINSERM UMR 941, Université de Paris Diderot, Paris, France; AP-HP, Hôpital Saint-Louis, Department of Infectious Diseases, Paris, FranceSorbonne Université, INSERM, Institut Pierre Louis d’Epidémiologie et de Santé Publique (iPLESP), AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de virologie, F-75013 Paris, FranceSorbonne Université, INSERM, Institut Pierre Louis d’Epidémiologie et de Santé Publique (iPLESP), AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de virologie, F-75013 Paris, France; Corresponding author at: Department of Virology, Bât CERVI, Hôpital Pitié-Salpêtrière, 83 Bd de l’Hôpital, 75013 Paris, France.Objectives: Presence of baseline hepatitis C virus (HCV) resistance-associated substitutions (RASs) can impair treatment outcome of direct-acting antivirals. We investigated the prevalence of pre-treatment HCV resistance among recently HCV-infected men who have sex with men (MSM) with high risk behaviours, either human immunodeficiency virus (HIV) co-infected or at high risk of HIV acquisition and under pre-exposure prophylaxis (PrEP). Methods: NS5A and NS3 fragments were deep sequenced on pre-treatment samples of 72 subjects using Illumina MiSeq paired-end sequencing technology. Ultra-deep sequencing data were analysed by SmartGene® platform. RASs mentioned in the literature were analysed and interpreted depending on genotype (GT) at 10% cut-off. Results: HCV genotyping showed 36 (50.0%) GT1a, 31 (43.1%) GT4d and 5 (6.9%) GT3a infections. Fifty-five patients (76.4%) were co-infected with HIV and 15 (20.8%) received PrEP. In GT1a viruses, NS3 RASs were found in 4/30 viruses (13.3%; S122 G/N, R155 K and I170 V) and Q80 K polymorphism was present in 14/30 viruses (46.7%). No NS3 RASs were detected in GT4d and GT3a viruses. NS5A RASs were detected in 3/36 GT1a viruses (8.3%; Q30E/R, L31 M and H58 L). NS5A subtype-specific polymorphisms L30R and T58 P were found at high frequencies in 31/31 (100%) and 16/31 (51.6%) GT4d viruses, respectively. One RAS M31 L was also observed along with the polymorphisms L30R and T58 P. No NS5A RASs were detected in GT3a viruses. Conclusion: A low level of RASs to NS3 and NS5A inhibitors in pre-treatment samples was detected in the study population. Our findings reassure the clinical management of HCV infection in this high-risk population.http://www.sciencedirect.com/science/article/pii/S2213716521000242Men who have sex with menDeep sequencingHepatitis C virusHCVResistance transmissionPre-exposure prophylaxis |