Study on human chondrocyte culture viability for autologous transplantation in clinical application
Objective: The limited regenerative capacity of the cartilage tissuemakes the treatment of chondral lesions difficult. The techniquescurrently available to treat cartilage lesions may relieve symptoms,but do not regenerate the injured tissue. Autologous chondrocytetransplantation uses cell biology a...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Instituto Israelita de Ensino e Pesquisa Albert Einstein
2003-06-01
|
Series: | Einstein (São Paulo) |
Subjects: | |
Online Access: | http://www.einstein.br/biblioteca/artigos/Study%20on%20human%20original.pdf |
id |
doaj-182a6b5a4cc544278cfde966b53ca788 |
---|---|
record_format |
Article |
spelling |
doaj-182a6b5a4cc544278cfde966b53ca7882020-11-24T20:43:53ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)1679-45082003-06-01128488Study on human chondrocyte culture viability for autologous transplantation in clinical applicationChristiane LombelloGeraldo Marins dos Reis JrMoisés CohenObjective: The limited regenerative capacity of the cartilage tissuemakes the treatment of chondral lesions difficult. The techniquescurrently available to treat cartilage lesions may relieve symptoms,but do not regenerate the injured tissue. Autologous chondrocytetransplantation uses cell biology and cell culture techniques toregenerate the hyaline cartilage. Methods: In this study, we analyzechondrocyte biopsy collection and culture for autologoustransplantation. Ultrastructural analyses of hyaline cartilage biopsieswere performed 0, 6, 24 and 48 hours after collection. The tissue evenafter 48 hours. Eleven cell culture assays were performed to evaluateisolation, viability, morphology, proliferation and absence ofcontaminants. Results: The cell culture techniques used allowedchondrocyte proliferation. Rates on cell viability were maintained abovethe acceptable patterns (above 90). Control of cell culture laboratoryconditions showed absence of contaminants, assuring safety of theprocess. The chondrocytes obtained presented the morphology typicalof cultured cell monolayers. Conclusion: The results indicate viabilityof chondrocyte culture technique for clinical application in autologoustransplantation.http://www.einstein.br/biblioteca/artigos/Study%20on%20human%20original.pdfCell cultureChondrocytes/transplantationTransplantationautologous/methodsCartilage |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christiane Lombello Geraldo Marins dos Reis Jr Moisés Cohen |
spellingShingle |
Christiane Lombello Geraldo Marins dos Reis Jr Moisés Cohen Study on human chondrocyte culture viability for autologous transplantation in clinical application Einstein (São Paulo) Cell culture Chondrocytes/transplantation Transplantation autologous/methods Cartilage |
author_facet |
Christiane Lombello Geraldo Marins dos Reis Jr Moisés Cohen |
author_sort |
Christiane Lombello |
title |
Study on human chondrocyte culture viability for autologous transplantation in clinical application |
title_short |
Study on human chondrocyte culture viability for autologous transplantation in clinical application |
title_full |
Study on human chondrocyte culture viability for autologous transplantation in clinical application |
title_fullStr |
Study on human chondrocyte culture viability for autologous transplantation in clinical application |
title_full_unstemmed |
Study on human chondrocyte culture viability for autologous transplantation in clinical application |
title_sort |
study on human chondrocyte culture viability for autologous transplantation in clinical application |
publisher |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
series |
Einstein (São Paulo) |
issn |
1679-4508 |
publishDate |
2003-06-01 |
description |
Objective: The limited regenerative capacity of the cartilage tissuemakes the treatment of chondral lesions difficult. The techniquescurrently available to treat cartilage lesions may relieve symptoms,but do not regenerate the injured tissue. Autologous chondrocytetransplantation uses cell biology and cell culture techniques toregenerate the hyaline cartilage. Methods: In this study, we analyzechondrocyte biopsy collection and culture for autologoustransplantation. Ultrastructural analyses of hyaline cartilage biopsieswere performed 0, 6, 24 and 48 hours after collection. The tissue evenafter 48 hours. Eleven cell culture assays were performed to evaluateisolation, viability, morphology, proliferation and absence ofcontaminants. Results: The cell culture techniques used allowedchondrocyte proliferation. Rates on cell viability were maintained abovethe acceptable patterns (above 90). Control of cell culture laboratoryconditions showed absence of contaminants, assuring safety of theprocess. The chondrocytes obtained presented the morphology typicalof cultured cell monolayers. Conclusion: The results indicate viabilityof chondrocyte culture technique for clinical application in autologoustransplantation. |
topic |
Cell culture Chondrocytes/transplantation Transplantation autologous/methods Cartilage |
url |
http://www.einstein.br/biblioteca/artigos/Study%20on%20human%20original.pdf |
work_keys_str_mv |
AT christianelombello studyonhumanchondrocytecultureviabilityforautologoustransplantationinclinicalapplication AT geraldomarinsdosreisjr studyonhumanchondrocytecultureviabilityforautologoustransplantationinclinicalapplication AT moisescohen studyonhumanchondrocytecultureviabilityforautologoustransplantationinclinicalapplication |
_version_ |
1716818666003103744 |