Hepatoprotective Effects of Steamed and Freeze-Dried Mature Silkworm Larval Powder against Ethanol-Induced Fatty Liver Disease in Rats
Silkworm, <i>Bombyx mori</i>, contains high amounts of beneficial nutrients, including amino acids, proteins, essential minerals, and omega-3 fatty acids. We have previously reported a technique for producing steamed and freeze-dried mature silkworm larval powder (SMSP), which makes it e...
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doaj-1834c0be4cc9480dae63dc6b0bb0ccf82020-11-25T02:15:07ZengMDPI AGFoods2304-81582020-03-019328510.3390/foods9030285foods9030285Hepatoprotective Effects of Steamed and Freeze-Dried Mature Silkworm Larval Powder against Ethanol-Induced Fatty Liver Disease in RatsDa-Young Lee0Kyung-Sook Hong1Moon-Young Song2Sun-Mi Yun3Sang-Deok Ji4Jong-Gon Son5Eun-Hee Kim6College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam 13488, KoreaCollege of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam 13488, KoreaCollege of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam 13488, KoreaCollege of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam 13488, KoreaDepartment of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, KoreaDepartment of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, KoreaCollege of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam 13488, KoreaSilkworm, <i>Bombyx mori</i>, contains high amounts of beneficial nutrients, including amino acids, proteins, essential minerals, and omega-3 fatty acids. We have previously reported a technique for producing steamed and freeze-dried mature silkworm larval powder (SMSP), which makes it easier to digest mature silkworm. In this study, we investigated the preventive effects of SMSP on alcoholic fatty liver disease and elucidated its mechanism of action. Male Sprague-Dawley rats treated with SMSP (50 mg/kg) or normal diet (AIN-76A) were administered 25% ethanol (3 g/kg body weight) by oral gavage for 4 weeks. SMSP administration for 4 weeks significantly decreased hepatic fat accumulation in ethanol-treated rats by modulating lipogenesis and fatty acid oxidation-related molecules such as sirtuin 1, AMP-activated protein kinase, and acetyl-CoA carboxylase 1. Moreover, SMSP administration significantly diminished the levels of triglyceride in liver tissues by as much as 35%, as well as lowering the serum levels of triglyceride, gamma glutamyl transpeptidase, alanine transaminase, and aspartate aminotransferase in ethanol-treated rats. SMSP supplementation also decreased the pro-inflammatory tumor necrosis factor-alpha and interleukin 1 beta levels and cytochrome P450 2E1 generating oxidative stress. These results suggest that SMSP administration may be possible for the prevention of alcoholic liver disease.https://www.mdpi.com/2304-8158/9/3/285steamed and freeze-dried mature silkworm larval powderalcoholic fatty liverethanollipogenesisfatty acid oxidationsprague-dawley rats |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Da-Young Lee Kyung-Sook Hong Moon-Young Song Sun-Mi Yun Sang-Deok Ji Jong-Gon Son Eun-Hee Kim |
spellingShingle |
Da-Young Lee Kyung-Sook Hong Moon-Young Song Sun-Mi Yun Sang-Deok Ji Jong-Gon Son Eun-Hee Kim Hepatoprotective Effects of Steamed and Freeze-Dried Mature Silkworm Larval Powder against Ethanol-Induced Fatty Liver Disease in Rats Foods steamed and freeze-dried mature silkworm larval powder alcoholic fatty liver ethanol lipogenesis fatty acid oxidation sprague-dawley rats |
author_facet |
Da-Young Lee Kyung-Sook Hong Moon-Young Song Sun-Mi Yun Sang-Deok Ji Jong-Gon Son Eun-Hee Kim |
author_sort |
Da-Young Lee |
title |
Hepatoprotective Effects of Steamed and Freeze-Dried Mature Silkworm Larval Powder against Ethanol-Induced Fatty Liver Disease in Rats |
title_short |
Hepatoprotective Effects of Steamed and Freeze-Dried Mature Silkworm Larval Powder against Ethanol-Induced Fatty Liver Disease in Rats |
title_full |
Hepatoprotective Effects of Steamed and Freeze-Dried Mature Silkworm Larval Powder against Ethanol-Induced Fatty Liver Disease in Rats |
title_fullStr |
Hepatoprotective Effects of Steamed and Freeze-Dried Mature Silkworm Larval Powder against Ethanol-Induced Fatty Liver Disease in Rats |
title_full_unstemmed |
Hepatoprotective Effects of Steamed and Freeze-Dried Mature Silkworm Larval Powder against Ethanol-Induced Fatty Liver Disease in Rats |
title_sort |
hepatoprotective effects of steamed and freeze-dried mature silkworm larval powder against ethanol-induced fatty liver disease in rats |
publisher |
MDPI AG |
series |
Foods |
issn |
2304-8158 |
publishDate |
2020-03-01 |
description |
Silkworm, <i>Bombyx mori</i>, contains high amounts of beneficial nutrients, including amino acids, proteins, essential minerals, and omega-3 fatty acids. We have previously reported a technique for producing steamed and freeze-dried mature silkworm larval powder (SMSP), which makes it easier to digest mature silkworm. In this study, we investigated the preventive effects of SMSP on alcoholic fatty liver disease and elucidated its mechanism of action. Male Sprague-Dawley rats treated with SMSP (50 mg/kg) or normal diet (AIN-76A) were administered 25% ethanol (3 g/kg body weight) by oral gavage for 4 weeks. SMSP administration for 4 weeks significantly decreased hepatic fat accumulation in ethanol-treated rats by modulating lipogenesis and fatty acid oxidation-related molecules such as sirtuin 1, AMP-activated protein kinase, and acetyl-CoA carboxylase 1. Moreover, SMSP administration significantly diminished the levels of triglyceride in liver tissues by as much as 35%, as well as lowering the serum levels of triglyceride, gamma glutamyl transpeptidase, alanine transaminase, and aspartate aminotransferase in ethanol-treated rats. SMSP supplementation also decreased the pro-inflammatory tumor necrosis factor-alpha and interleukin 1 beta levels and cytochrome P450 2E1 generating oxidative stress. These results suggest that SMSP administration may be possible for the prevention of alcoholic liver disease. |
topic |
steamed and freeze-dried mature silkworm larval powder alcoholic fatty liver ethanol lipogenesis fatty acid oxidation sprague-dawley rats |
url |
https://www.mdpi.com/2304-8158/9/3/285 |
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