Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)
The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm mo...
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Colégio Brasileiro de Patologia Animal (CBPA)
2013-07-01
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doaj-183a3c3a6525416f950662ff86acd1d02020-11-25T01:32:40ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-51502013-07-0133792493010.1590/S0100-736X2013000700014S0100-736X2013000700014Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)Patrícia C. Sousa0Erika A.A. Santos1Ana L.P. Souza2Gabriela L. Lima3Felipe F.P.C. Barros4Moacir F. Oliveira5Alexandre R. Silva6Universidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoThe aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013000700014&lng=en&tlng=enPecari tajacucoloraçãomorfologia espermáticamorfometria espermática |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Patrícia C. Sousa Erika A.A. Santos Ana L.P. Souza Gabriela L. Lima Felipe F.P.C. Barros Moacir F. Oliveira Alexandre R. Silva |
spellingShingle |
Patrícia C. Sousa Erika A.A. Santos Ana L.P. Souza Gabriela L. Lima Felipe F.P.C. Barros Moacir F. Oliveira Alexandre R. Silva Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu) Pesquisa Veterinária Brasileira Pecari tajacu coloração morfologia espermática morfometria espermática |
author_facet |
Patrícia C. Sousa Erika A.A. Santos Ana L.P. Souza Gabriela L. Lima Felipe F.P.C. Barros Moacir F. Oliveira Alexandre R. Silva |
author_sort |
Patrícia C. Sousa |
title |
Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu) |
title_short |
Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu) |
title_full |
Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu) |
title_fullStr |
Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu) |
title_full_unstemmed |
Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu) |
title_sort |
sperm morphological and morphometric evaluation in captive collared peccaries (pecari tajacu) |
publisher |
Colégio Brasileiro de Patologia Animal (CBPA) |
series |
Pesquisa Veterinária Brasileira |
issn |
1678-5150 |
publishDate |
2013-07-01 |
description |
The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm. |
topic |
Pecari tajacu coloração morfologia espermática morfometria espermática |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013000700014&lng=en&tlng=en |
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