The effects of L-Arginine supplementation on growth performance and intestinal health of broiler chickens challenged with Eimeria spp.
This study evaluated the effects of varying levels of L-arginine (Arg) on performance and intestinal health of broilers challenged with Eimeria. Cobb 500 male chicks (n = 720) were randomly distributed in a 5 × 2 factorial arrangement (6 replicates/12 birds). The main factors were Arg levels (1.04,...
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doaj-183dd811aacd4866abef40fff302d94f2020-11-25T03:35:59ZengElsevierPoultry Science0032-57912020-11-01991158445857The effects of L-Arginine supplementation on growth performance and intestinal health of broiler chickens challenged with Eimeria spp.Fernanda L.S. Castro0Po-Yun Teng1Sudhir Yadav2Rebecca L. Gould3Steven Craig4Robert Pazdro5Woo K. Kim6Department of Poultry Science, University of Georgia, Athens, GA, 30602, USADepartment of Poultry Science, University of Georgia, Athens, GA, 30602, USADepartment of Poultry Science, University of Georgia, Athens, GA, 30602, USADepartment of Foods and Nutrition, University of Georgia (UGA), Athens, GA, USADepartment of Foods and Nutrition, University of Georgia (UGA), Athens, GA, USADepartment of Foods and Nutrition, University of Georgia (UGA), Athens, GA, USADepartment of Poultry Science, University of Georgia, Athens, GA, 30602, USA; Corresponding author:This study evaluated the effects of varying levels of L-arginine (Arg) on performance and intestinal health of broilers challenged with Eimeria. Cobb 500 male chicks (n = 720) were randomly distributed in a 5 × 2 factorial arrangement (6 replicates/12 birds). The main factors were Arg levels (1.04, 1.14, 1.24, 1.34, 1.44%) and challenge or non-challenge with Eimeria. At day 12, in the challenge group, each bird received orally 12,500 Eimeria maxima, 12,500 Eimeria tenella, and 62,500 Eimeria acervulina sporulated oocysts. At 5 d postinfection (dpi), intestinal permeability was measured. At 6 and 14 dpi, performance, intestinal histomorphology, nutrient digestibility, tight junction protein (TJP) gene expression, and antioxidant markers were evaluated. Few interactions were found, and when significant, the supplementation of Arg did not counteract the negative effects of Eimeria challenge. Challenge, regardless of Arg level, increased intestinal permeability, although the expression of Claudin-1, a TJP, was upregulated. At 6 dpi, the antioxidant system was impaired by the challenge. Moreover, growth performance, intestinal histomorphology, and nutrient digestibility were negatively affected by challenge at 6 and 14 dpi. Regardless of challenge, from 0 to 14 dpi, birds fed 1.44% showed higher weight gain than 1.04% of Arg, and birds fed 1.34% showed lower feed conversion than 1.04% of Arg. At 5 dpi, intestinal permeability was improved in birds fed 1.34% than 1.04% of Arg. Moreover, 1.34% of Arg upregulated the expression of the TJP Zonula occludens-1 (ZO-1) as compared with 1.24 and 1.44% of Arg at 6 dpi. At 14 dpi, 1.44% of Arg upregulated the expression of ZO-1 and ZO-2 compared with 1.24 and 1.34% of Arg. The nutrient digestibility was quadratically influenced by Arg, whereas the antioxidant markers were unaffected. Thus, the challenge with Eimeria had a negative impact on growth and intestinal health. The dietary supplementation of levels ranging from 1.24 to 1.44% of Arg showed promising results, improving overall growth, intestinal integrity, and morphology in broilers subjected or not to Eimeria challenge.http://www.sciencedirect.com/science/article/pii/S0032579120305551L-argininebroilerEimeriaintestinal health |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernanda L.S. Castro Po-Yun Teng Sudhir Yadav Rebecca L. Gould Steven Craig Robert Pazdro Woo K. Kim |
spellingShingle |
Fernanda L.S. Castro Po-Yun Teng Sudhir Yadav Rebecca L. Gould Steven Craig Robert Pazdro Woo K. Kim The effects of L-Arginine supplementation on growth performance and intestinal health of broiler chickens challenged with Eimeria spp. Poultry Science L-arginine broiler Eimeria intestinal health |
author_facet |
Fernanda L.S. Castro Po-Yun Teng Sudhir Yadav Rebecca L. Gould Steven Craig Robert Pazdro Woo K. Kim |
author_sort |
Fernanda L.S. Castro |
title |
The effects of L-Arginine supplementation on growth performance and intestinal health of broiler chickens challenged with Eimeria spp. |
title_short |
The effects of L-Arginine supplementation on growth performance and intestinal health of broiler chickens challenged with Eimeria spp. |
title_full |
The effects of L-Arginine supplementation on growth performance and intestinal health of broiler chickens challenged with Eimeria spp. |
title_fullStr |
The effects of L-Arginine supplementation on growth performance and intestinal health of broiler chickens challenged with Eimeria spp. |
title_full_unstemmed |
The effects of L-Arginine supplementation on growth performance and intestinal health of broiler chickens challenged with Eimeria spp. |
title_sort |
effects of l-arginine supplementation on growth performance and intestinal health of broiler chickens challenged with eimeria spp. |
publisher |
Elsevier |
series |
Poultry Science |
issn |
0032-5791 |
publishDate |
2020-11-01 |
description |
This study evaluated the effects of varying levels of L-arginine (Arg) on performance and intestinal health of broilers challenged with Eimeria. Cobb 500 male chicks (n = 720) were randomly distributed in a 5 × 2 factorial arrangement (6 replicates/12 birds). The main factors were Arg levels (1.04, 1.14, 1.24, 1.34, 1.44%) and challenge or non-challenge with Eimeria. At day 12, in the challenge group, each bird received orally 12,500 Eimeria maxima, 12,500 Eimeria tenella, and 62,500 Eimeria acervulina sporulated oocysts. At 5 d postinfection (dpi), intestinal permeability was measured. At 6 and 14 dpi, performance, intestinal histomorphology, nutrient digestibility, tight junction protein (TJP) gene expression, and antioxidant markers were evaluated. Few interactions were found, and when significant, the supplementation of Arg did not counteract the negative effects of Eimeria challenge. Challenge, regardless of Arg level, increased intestinal permeability, although the expression of Claudin-1, a TJP, was upregulated. At 6 dpi, the antioxidant system was impaired by the challenge. Moreover, growth performance, intestinal histomorphology, and nutrient digestibility were negatively affected by challenge at 6 and 14 dpi. Regardless of challenge, from 0 to 14 dpi, birds fed 1.44% showed higher weight gain than 1.04% of Arg, and birds fed 1.34% showed lower feed conversion than 1.04% of Arg. At 5 dpi, intestinal permeability was improved in birds fed 1.34% than 1.04% of Arg. Moreover, 1.34% of Arg upregulated the expression of the TJP Zonula occludens-1 (ZO-1) as compared with 1.24 and 1.44% of Arg at 6 dpi. At 14 dpi, 1.44% of Arg upregulated the expression of ZO-1 and ZO-2 compared with 1.24 and 1.34% of Arg. The nutrient digestibility was quadratically influenced by Arg, whereas the antioxidant markers were unaffected. Thus, the challenge with Eimeria had a negative impact on growth and intestinal health. The dietary supplementation of levels ranging from 1.24 to 1.44% of Arg showed promising results, improving overall growth, intestinal integrity, and morphology in broilers subjected or not to Eimeria challenge. |
topic |
L-arginine broiler Eimeria intestinal health |
url |
http://www.sciencedirect.com/science/article/pii/S0032579120305551 |
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