Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions
In this study, we aimed to compare the morphogenetic andneuronal characteristics between monolayer cells andspheroids. For this purpose, we established spheroid formationby growing SH-SY5Y cells on the hydrophobic surfaces ofthermally-collapsed elastin-like polypeptide. After 4 days ofculture, the r...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Korean Society for Biochemistry and Molecular Biology
2013-05-01
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Series: | BMB Reports |
Subjects: | |
Online Access: | http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTFAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni01JTVEMTMwNTI5MjI0MV8lMjgyNzYtMjgxJTI5Qk1CXzEyLTE5Ni5wZGY= |
Summary: | In this study, we aimed to compare the morphogenetic andneuronal characteristics between monolayer cells andspheroids. For this purpose, we established spheroid formationby growing SH-SY5Y cells on the hydrophobic surfaces ofthermally-collapsed elastin-like polypeptide. After 4 days ofculture, the relative proliferation of the cells within spheroidswas approximately 92% of the values for monolayer cultures.As measured by quantitative assays for mRNA and proteinexpressions, the production of synaptophysin and neuronspecificenolase (NSE) as well as the contents of cell adhesionmolecules (CAMs) and extracellular matrix (ECM) proteins aremuch higher in spheroids than in monolayer cells. Under theall-trans-retinoic acid (RA)-induced differentiation condition,spheroids extended neurites and further up-regulated theexpression of synaptophysin, NSE, CAMs, and ECM proteins.Our data indicate that RA-differentiated SH-SY5Y neurospheroidsare functionally matured neuronal architectures. [BMBReports 2013; 46(5): 276-281] |
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ISSN: | 1976-6696 1976-670X |