Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions
In this study, we aimed to compare the morphogenetic andneuronal characteristics between monolayer cells andspheroids. For this purpose, we established spheroid formationby growing SH-SY5Y cells on the hydrophobic surfaces ofthermally-collapsed elastin-like polypeptide. After 4 days ofculture, the r...
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Korean Society for Biochemistry and Molecular Biology
2013-05-01
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doaj-184576ec99f94b7f8dfaab153a3ec61e2020-11-24T22:46:59ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2013-05-01465276281http://dx.doi.org/10.5483/BMBRep.2013.46.5.196Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditionsGwon-Soo JungKyeong-Min LeeJin-Kyu ParkSeong-Kyoon ChoiWon Bae JeonIn this study, we aimed to compare the morphogenetic andneuronal characteristics between monolayer cells andspheroids. For this purpose, we established spheroid formationby growing SH-SY5Y cells on the hydrophobic surfaces ofthermally-collapsed elastin-like polypeptide. After 4 days ofculture, the relative proliferation of the cells within spheroidswas approximately 92% of the values for monolayer cultures.As measured by quantitative assays for mRNA and proteinexpressions, the production of synaptophysin and neuronspecificenolase (NSE) as well as the contents of cell adhesionmolecules (CAMs) and extracellular matrix (ECM) proteins aremuch higher in spheroids than in monolayer cells. Under theall-trans-retinoic acid (RA)-induced differentiation condition,spheroids extended neurites and further up-regulated theexpression of synaptophysin, NSE, CAMs, and ECM proteins.Our data indicate that RA-differentiated SH-SY5Y neurospheroidsare functionally matured neuronal architectures. [BMBReports 2013; 46(5): 276-281]http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTFAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni01JTVEMTMwNTI5MjI0MV8lMjgyNzYtMjgxJTI5Qk1CXzEyLTE5Ni5wZGY=Cell adhesion moleculesExtracellular matrixMulticellular neurospheroidsNeuroblastoma cellsNeuronal differentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gwon-Soo Jung Kyeong-Min Lee Jin-Kyu Park Seong-Kyoon Choi Won Bae Jeon |
spellingShingle |
Gwon-Soo Jung Kyeong-Min Lee Jin-Kyu Park Seong-Kyoon Choi Won Bae Jeon Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions BMB Reports Cell adhesion molecules Extracellular matrix Multicellular neurospheroids Neuroblastoma cells Neuronal differentiation |
author_facet |
Gwon-Soo Jung Kyeong-Min Lee Jin-Kyu Park Seong-Kyoon Choi Won Bae Jeon |
author_sort |
Gwon-Soo Jung |
title |
Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions |
title_short |
Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions |
title_full |
Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions |
title_fullStr |
Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions |
title_full_unstemmed |
Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions |
title_sort |
morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions |
publisher |
Korean Society for Biochemistry and Molecular Biology |
series |
BMB Reports |
issn |
1976-6696 1976-670X |
publishDate |
2013-05-01 |
description |
In this study, we aimed to compare the morphogenetic andneuronal characteristics between monolayer cells andspheroids. For this purpose, we established spheroid formationby growing SH-SY5Y cells on the hydrophobic surfaces ofthermally-collapsed elastin-like polypeptide. After 4 days ofculture, the relative proliferation of the cells within spheroidswas approximately 92% of the values for monolayer cultures.As measured by quantitative assays for mRNA and proteinexpressions, the production of synaptophysin and neuronspecificenolase (NSE) as well as the contents of cell adhesionmolecules (CAMs) and extracellular matrix (ECM) proteins aremuch higher in spheroids than in monolayer cells. Under theall-trans-retinoic acid (RA)-induced differentiation condition,spheroids extended neurites and further up-regulated theexpression of synaptophysin, NSE, CAMs, and ECM proteins.Our data indicate that RA-differentiated SH-SY5Y neurospheroidsare functionally matured neuronal architectures. [BMBReports 2013; 46(5): 276-281] |
topic |
Cell adhesion molecules Extracellular matrix Multicellular neurospheroids Neuroblastoma cells Neuronal differentiation |
url |
http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTFAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni01JTVEMTMwNTI5MjI0MV8lMjgyNzYtMjgxJTI5Qk1CXzEyLTE5Ni5wZGY= |
work_keys_str_mv |
AT gwonsoojung morphogeneticandneuronalcharacterizationofhumanneuroblastomamulticellularspheroidsculturedunderundifferentiatedandalltransretinoicaciddifferentiatedconditions AT kyeongminlee morphogeneticandneuronalcharacterizationofhumanneuroblastomamulticellularspheroidsculturedunderundifferentiatedandalltransretinoicaciddifferentiatedconditions AT jinkyupark morphogeneticandneuronalcharacterizationofhumanneuroblastomamulticellularspheroidsculturedunderundifferentiatedandalltransretinoicaciddifferentiatedconditions AT seongkyoonchoi morphogeneticandneuronalcharacterizationofhumanneuroblastomamulticellularspheroidsculturedunderundifferentiatedandalltransretinoicaciddifferentiatedconditions AT wonbaejeon morphogeneticandneuronalcharacterizationofhumanneuroblastomamulticellularspheroidsculturedunderundifferentiatedandalltransretinoicaciddifferentiatedconditions |
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1725682868182581248 |