Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system
Abstract The type VI secretion system (T6SS) is a multiprotein weapon that kills eukaryotic predators or prokaryotic competitors by delivering toxic effectors. Despite the importance of T6SS in bacterial environmental adaptation, it is still challenging to systematically identify T6SS effectors beca...
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doaj-184c9f695ed048d08e9d10d483b9642f2020-11-25T03:21:55ZengBMCVeterinary Research1297-97162020-05-0151111410.1186/s13567-020-00794-wIdentification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion systemShuiyan Ma0Yuhao Dong1Nannan Wang2Jin Liu3Chengping Lu4Yongjie Liu5Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Jinling Institute of TechnologyJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural UniversityAbstract The type VI secretion system (T6SS) is a multiprotein weapon that kills eukaryotic predators or prokaryotic competitors by delivering toxic effectors. Despite the importance of T6SS in bacterial environmental adaptation, it is still challenging to systematically identify T6SS effectors because of their high diversity and lack of conserved domains. In this report, we discovered a putative effector gene, U876-17730, in the whole genome of Aeromonas hydrophila NJ-35 based on the reported conservative domain DUF4123 (domain of unknown function), with two cognate immunity proteins encoded downstream. Phylogenetic tree analysis of amino acids indicates that AH17730 belongs to the Tle1 (type VI lipase effector) family, and therefore was named Tle1AH. The deletion of tle1 AH resulted in significantly decreased biofilm formation, antibacterial competition ability and virulence in zebrafish (Danio rerio) when compared to the wild-type strain. Only when the two immunity proteins coexist can bacteria protect themselves from the toxicity of Tle1AH. Further study shows that Tle1AH is a kind of phospholipase that possesses a conserved lipase motif, Gly-X-Ser-X-Gly (X is for any amino acid). Tle1AH is secreted by T6SS, and this secretion requires its interaction with an associated VgrG (valine-glycine repeat protein G). In conclusion, we identified a T6SS effector-immunity pair and verified its function, which lays the foundation for future research on the role of T6SS in the pathogenic mechanism of A. hydrophila.http://link.springer.com/article/10.1186/s13567-020-00794-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuiyan Ma Yuhao Dong Nannan Wang Jin Liu Chengping Lu Yongjie Liu |
spellingShingle |
Shuiyan Ma Yuhao Dong Nannan Wang Jin Liu Chengping Lu Yongjie Liu Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system Veterinary Research |
author_facet |
Shuiyan Ma Yuhao Dong Nannan Wang Jin Liu Chengping Lu Yongjie Liu |
author_sort |
Shuiyan Ma |
title |
Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system |
title_short |
Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system |
title_full |
Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system |
title_fullStr |
Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system |
title_full_unstemmed |
Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system |
title_sort |
identification of a new effector-immunity pair of aeromonas hydrophila type vi secretion system |
publisher |
BMC |
series |
Veterinary Research |
issn |
1297-9716 |
publishDate |
2020-05-01 |
description |
Abstract The type VI secretion system (T6SS) is a multiprotein weapon that kills eukaryotic predators or prokaryotic competitors by delivering toxic effectors. Despite the importance of T6SS in bacterial environmental adaptation, it is still challenging to systematically identify T6SS effectors because of their high diversity and lack of conserved domains. In this report, we discovered a putative effector gene, U876-17730, in the whole genome of Aeromonas hydrophila NJ-35 based on the reported conservative domain DUF4123 (domain of unknown function), with two cognate immunity proteins encoded downstream. Phylogenetic tree analysis of amino acids indicates that AH17730 belongs to the Tle1 (type VI lipase effector) family, and therefore was named Tle1AH. The deletion of tle1 AH resulted in significantly decreased biofilm formation, antibacterial competition ability and virulence in zebrafish (Danio rerio) when compared to the wild-type strain. Only when the two immunity proteins coexist can bacteria protect themselves from the toxicity of Tle1AH. Further study shows that Tle1AH is a kind of phospholipase that possesses a conserved lipase motif, Gly-X-Ser-X-Gly (X is for any amino acid). Tle1AH is secreted by T6SS, and this secretion requires its interaction with an associated VgrG (valine-glycine repeat protein G). In conclusion, we identified a T6SS effector-immunity pair and verified its function, which lays the foundation for future research on the role of T6SS in the pathogenic mechanism of A. hydrophila. |
url |
http://link.springer.com/article/10.1186/s13567-020-00794-w |
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