Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in Vivo
The report aims to investigate the relationship between the expression of cyclin D1 and Cyclooxgenase-2 (COX-2), thus to explore the molecular mechanisms of the antitumor efficacy of Celecoxib, a COX-2 inhibitor. Human ovarian SKOV-3 carcinoma cell xenograft-bearing mice were treated with Celecoxib...
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doaj-185188ee480f41e58bc2db53be1177862020-11-25T00:27:33ZengMDPI AGInternational Journal of Molecular Sciences1422-00672010-10-0111103999401310.3390/ijms11103999Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in VivoLing-Yun ZhaiMei-Lin LiuXiao-Li XuJun ZhangJie WangHong-Ru JiangWei LiThe report aims to investigate the relationship between the expression of cyclin D1 and Cyclooxgenase-2 (COX-2), thus to explore the molecular mechanisms of the antitumor efficacy of Celecoxib, a COX-2 inhibitor. Human ovarian SKOV-3 carcinoma cell xenograft-bearing mice were treated with Celecoxib by infusing gaster (i.g.) twice/day for 21 days. The mRNA levels of COX-2 and cyclin D1 were determined by RT-PCR. The expression of cyclin D1 at the protein level was detected by immunohistochemistry, while COX-2 protein expression was determined by Western blot. A high-dose of Celecoxib (100 mg/kg) significantly inhibited tumor growth (P < 0.05), and the expression of cyclin D1 was reduced by 61%. Celecoxib decreased the proliferation cell index by 40% (P < 0.001) and increased apoptotic index by 52% (P < 0.05) in high-dose Celecoxib treated group. Our results suggest that the antitumor efficacy of Celecoxib against ovarian cancer in mice may in part be mediated through suppression of cyclin D1, which may contribute to its ability to suppress proliferation. http://www.mdpi.com/1422-0067/11/10/3999/Celecoxibcyclin D1ovarian cancerproliferationapoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ling-Yun Zhai Mei-Lin Liu Xiao-Li Xu Jun Zhang Jie Wang Hong-Ru Jiang Wei Li |
spellingShingle |
Ling-Yun Zhai Mei-Lin Liu Xiao-Li Xu Jun Zhang Jie Wang Hong-Ru Jiang Wei Li Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in Vivo International Journal of Molecular Sciences Celecoxib cyclin D1 ovarian cancer proliferation apoptosis |
author_facet |
Ling-Yun Zhai Mei-Lin Liu Xiao-Li Xu Jun Zhang Jie Wang Hong-Ru Jiang Wei Li |
author_sort |
Ling-Yun Zhai |
title |
Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in Vivo |
title_short |
Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in Vivo |
title_full |
Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in Vivo |
title_fullStr |
Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in Vivo |
title_full_unstemmed |
Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in Vivo |
title_sort |
cyclin d1 expression and the inhibitory effect of celecoxib on ovarian tumor growth in vivo |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2010-10-01 |
description |
The report aims to investigate the relationship between the expression of cyclin D1 and Cyclooxgenase-2 (COX-2), thus to explore the molecular mechanisms of the antitumor efficacy of Celecoxib, a COX-2 inhibitor. Human ovarian SKOV-3 carcinoma cell xenograft-bearing mice were treated with Celecoxib by infusing gaster (i.g.) twice/day for 21 days. The mRNA levels of COX-2 and cyclin D1 were determined by RT-PCR. The expression of cyclin D1 at the protein level was detected by immunohistochemistry, while COX-2 protein expression was determined by Western blot. A high-dose of Celecoxib (100 mg/kg) significantly inhibited tumor growth (P < 0.05), and the expression of cyclin D1 was reduced by 61%. Celecoxib decreased the proliferation cell index by 40% (P < 0.001) and increased apoptotic index by 52% (P < 0.05) in high-dose Celecoxib treated group. Our results suggest that the antitumor efficacy of Celecoxib against ovarian cancer in mice may in part be mediated through suppression of cyclin D1, which may contribute to its ability to suppress proliferation. |
topic |
Celecoxib cyclin D1 ovarian cancer proliferation apoptosis |
url |
http://www.mdpi.com/1422-0067/11/10/3999/ |
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