Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus
Abstract Background Influenza A virus (IAV) infection is a continual threat to the health of animals and humans globally. Consumption of the conventional drugs has shown several side effects and drug resistance. This study was aimed to screen some Iranian medicinal plants extracts and their fraction...
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doaj-185827bb3bd74b1e929825b8cacefa022021-10-03T11:53:55ZengBMCBMC Complementary Medicine and Therapies2662-76712021-10-0121111210.1186/s12906-021-03423-xPotential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virusParvaneh Mehrbod0Hanieh Safari1Zeinab Mollai2Fatemeh Fotouhi3Yasaman Mirfakhraei4Hanieh Entezari5Saied Goodarzi6Zahra Tofighi7Influenza and Respiratory Viruses Department, Pasteur Institute of IRANDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical SciencesDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical SciencesInfluenza and Respiratory Viruses Department, Pasteur Institute of IRANDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical SciencesDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical SciencesMedicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical SciencesDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical SciencesAbstract Background Influenza A virus (IAV) infection is a continual threat to the health of animals and humans globally. Consumption of the conventional drugs has shown several side effects and drug resistance. This study was aimed to screen some Iranian medicinal plants extracts and their fractions against influenza A virus. Methods Glycyrrhiza glabra (rhizome), Myrtus commonis (leaves), Melissa officinalis (leaves), Hypericum perforatum (aerial parts), Tilia platyphyllos (flower), Salix alba (bark), and Camellia sinensis (green and fermented leaves) were extracted with 80% methanol and fractionated with chloroform and methanol, respectively. The cytotoxicity of the compounds were determined by MTT colorimetric assay on MDCK cells. The effective concentrations (EC50) of the compounds were calculated from the MTT results compared to the negative control with no significant effects on cell viability. The effects of EC50 of the compounds on viral surface glycoproteins and viral titer were tested by HI and HA virological assays, respectively and compared with oseltamivir and amantadine. Preliminary phytochemical analysis were done for promising anti-IAV extracts and fractions. Results The most effective samples against IAV titer (P ≤ 0.05) were crude extracts of G. glabra, M. officinalis and S. alba; methanol fractions of M. communis and M. officinalis; and chloroform fractions of M. communis and C. sinensis (fermented) mostly in co- and pre-penetration combined treatments. The potential extracts and fractions were rich in flavonoids, tannins, steroids and triterpenoids. Conclusion The outcomes confirmed a scientific basis for anti-influenza A virus capacity of the extracts and fractions from the selected plants for the first time, and correlated their effects with their phytochemical constituents. It is worth focusing on elucidating pure compounds and identifying their mechanism(s) of action.https://doi.org/10.1186/s12906-021-03423-xLicoriceLemon balmWhite willowMyrtleBlack teaAnti-influenza A viral activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Parvaneh Mehrbod Hanieh Safari Zeinab Mollai Fatemeh Fotouhi Yasaman Mirfakhraei Hanieh Entezari Saied Goodarzi Zahra Tofighi |
spellingShingle |
Parvaneh Mehrbod Hanieh Safari Zeinab Mollai Fatemeh Fotouhi Yasaman Mirfakhraei Hanieh Entezari Saied Goodarzi Zahra Tofighi Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus BMC Complementary Medicine and Therapies Licorice Lemon balm White willow Myrtle Black tea Anti-influenza A viral activity |
author_facet |
Parvaneh Mehrbod Hanieh Safari Zeinab Mollai Fatemeh Fotouhi Yasaman Mirfakhraei Hanieh Entezari Saied Goodarzi Zahra Tofighi |
author_sort |
Parvaneh Mehrbod |
title |
Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus |
title_short |
Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus |
title_full |
Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus |
title_fullStr |
Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus |
title_full_unstemmed |
Potential antiviral effects of some native Iranian medicinal plants extracts and fractions against influenza A virus |
title_sort |
potential antiviral effects of some native iranian medicinal plants extracts and fractions against influenza a virus |
publisher |
BMC |
series |
BMC Complementary Medicine and Therapies |
issn |
2662-7671 |
publishDate |
2021-10-01 |
description |
Abstract Background Influenza A virus (IAV) infection is a continual threat to the health of animals and humans globally. Consumption of the conventional drugs has shown several side effects and drug resistance. This study was aimed to screen some Iranian medicinal plants extracts and their fractions against influenza A virus. Methods Glycyrrhiza glabra (rhizome), Myrtus commonis (leaves), Melissa officinalis (leaves), Hypericum perforatum (aerial parts), Tilia platyphyllos (flower), Salix alba (bark), and Camellia sinensis (green and fermented leaves) were extracted with 80% methanol and fractionated with chloroform and methanol, respectively. The cytotoxicity of the compounds were determined by MTT colorimetric assay on MDCK cells. The effective concentrations (EC50) of the compounds were calculated from the MTT results compared to the negative control with no significant effects on cell viability. The effects of EC50 of the compounds on viral surface glycoproteins and viral titer were tested by HI and HA virological assays, respectively and compared with oseltamivir and amantadine. Preliminary phytochemical analysis were done for promising anti-IAV extracts and fractions. Results The most effective samples against IAV titer (P ≤ 0.05) were crude extracts of G. glabra, M. officinalis and S. alba; methanol fractions of M. communis and M. officinalis; and chloroform fractions of M. communis and C. sinensis (fermented) mostly in co- and pre-penetration combined treatments. The potential extracts and fractions were rich in flavonoids, tannins, steroids and triterpenoids. Conclusion The outcomes confirmed a scientific basis for anti-influenza A virus capacity of the extracts and fractions from the selected plants for the first time, and correlated their effects with their phytochemical constituents. It is worth focusing on elucidating pure compounds and identifying their mechanism(s) of action. |
topic |
Licorice Lemon balm White willow Myrtle Black tea Anti-influenza A viral activity |
url |
https://doi.org/10.1186/s12906-021-03423-x |
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