Danhong Inhibits Oxidized Low-Density Lipoprotein–Induced Immune Maturation of Dentritic Cells via a Peroxisome Proliferator Activated Receptor γ–Mediated Pathway
Danhong injection (DHI), a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is effective in the treatment of atherosclerosis (AS)-related diseases. It is widely recognized that AS is a complex inflammatory disease of the arterial wal...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2012-01-01
|
Series: | Journal of Pharmacological Sciences |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319305183 |
id |
doaj-185978a43a644c0292dfd2a398995177 |
---|---|
record_format |
Article |
spelling |
doaj-185978a43a644c0292dfd2a3989951772020-11-25T02:31:03ZengElsevierJournal of Pharmacological Sciences1347-86132012-01-01119119Danhong Inhibits Oxidized Low-Density Lipoprotein–Induced Immune Maturation of Dentritic Cells via a Peroxisome Proliferator Activated Receptor γ–Mediated PathwayHongying Liu0Shijun Wang1Aijun Sun2Dong Huang3Wei Wang4Chunyu Zhang5Dazhuo Shi6Keji Chen7Yunzeng Zou8Junbo Ge9Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR ChinaShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR ChinaShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR ChinaShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR ChinaShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR ChinaShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR ChinaXiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, PR ChinaXiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, PR ChinaShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR ChinaShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, PR China; Corresponding author. jbge@zs-hospital.sh.cnDanhong injection (DHI), a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is effective in the treatment of atherosclerosis (AS)-related diseases. It is widely recognized that AS is a complex inflammatory disease of the arterial wall and the dendritic cells (DCs) is a major player in the pathogenesis of AS via mediating atherosclerotic antigen presenting and T lymphocytes. Here, we determined the effect and possible mechanism of DHI on oxidized low-density lipoprotein (ox-LDL)-induced maturation and immune function of DCs. Human monocyte-derived DCs were incubated with DHI or ciglitazone and were subsequently stimulated with ox-LDL to induce maturation. Similar to ciglitazone, a peroxisome proliferator activated receptor (PPAR) γ agonist, DHI, could significantly reduce ox-LDL-induced expressions of mature markers, enhance the endocytotic function, and inhibit secretions of cytokine on DCs. These effects of DHI could be partly reversed by silencing the PPARγ. In conclusion, DHI could inhibit ox-LDL-induced maturation of DCs partly through activating a PPARγ-mediated signaling pathway.[Supplementary Methods and Figures: available only at http://dx.doi.org/10.1254/jphs.11226FP] Keywords:: Danhong injection, dendritic cell, peroxisome proliferator activated receptor (PPAR) γ, atherosclerosishttp://www.sciencedirect.com/science/article/pii/S1347861319305183 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hongying Liu Shijun Wang Aijun Sun Dong Huang Wei Wang Chunyu Zhang Dazhuo Shi Keji Chen Yunzeng Zou Junbo Ge |
spellingShingle |
Hongying Liu Shijun Wang Aijun Sun Dong Huang Wei Wang Chunyu Zhang Dazhuo Shi Keji Chen Yunzeng Zou Junbo Ge Danhong Inhibits Oxidized Low-Density Lipoprotein–Induced Immune Maturation of Dentritic Cells via a Peroxisome Proliferator Activated Receptor γ–Mediated Pathway Journal of Pharmacological Sciences |
author_facet |
Hongying Liu Shijun Wang Aijun Sun Dong Huang Wei Wang Chunyu Zhang Dazhuo Shi Keji Chen Yunzeng Zou Junbo Ge |
author_sort |
Hongying Liu |
title |
Danhong Inhibits Oxidized Low-Density Lipoprotein–Induced Immune Maturation of Dentritic Cells via a Peroxisome Proliferator Activated Receptor γ–Mediated Pathway |
title_short |
Danhong Inhibits Oxidized Low-Density Lipoprotein–Induced Immune Maturation of Dentritic Cells via a Peroxisome Proliferator Activated Receptor γ–Mediated Pathway |
title_full |
Danhong Inhibits Oxidized Low-Density Lipoprotein–Induced Immune Maturation of Dentritic Cells via a Peroxisome Proliferator Activated Receptor γ–Mediated Pathway |
title_fullStr |
Danhong Inhibits Oxidized Low-Density Lipoprotein–Induced Immune Maturation of Dentritic Cells via a Peroxisome Proliferator Activated Receptor γ–Mediated Pathway |
title_full_unstemmed |
Danhong Inhibits Oxidized Low-Density Lipoprotein–Induced Immune Maturation of Dentritic Cells via a Peroxisome Proliferator Activated Receptor γ–Mediated Pathway |
title_sort |
danhong inhibits oxidized low-density lipoprotein–induced immune maturation of dentritic cells via a peroxisome proliferator activated receptor γ–mediated pathway |
publisher |
Elsevier |
series |
Journal of Pharmacological Sciences |
issn |
1347-8613 |
publishDate |
2012-01-01 |
description |
Danhong injection (DHI), a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is effective in the treatment of atherosclerosis (AS)-related diseases. It is widely recognized that AS is a complex inflammatory disease of the arterial wall and the dendritic cells (DCs) is a major player in the pathogenesis of AS via mediating atherosclerotic antigen presenting and T lymphocytes. Here, we determined the effect and possible mechanism of DHI on oxidized low-density lipoprotein (ox-LDL)-induced maturation and immune function of DCs. Human monocyte-derived DCs were incubated with DHI or ciglitazone and were subsequently stimulated with ox-LDL to induce maturation. Similar to ciglitazone, a peroxisome proliferator activated receptor (PPAR) γ agonist, DHI, could significantly reduce ox-LDL-induced expressions of mature markers, enhance the endocytotic function, and inhibit secretions of cytokine on DCs. These effects of DHI could be partly reversed by silencing the PPARγ. In conclusion, DHI could inhibit ox-LDL-induced maturation of DCs partly through activating a PPARγ-mediated signaling pathway.[Supplementary Methods and Figures: available only at http://dx.doi.org/10.1254/jphs.11226FP] Keywords:: Danhong injection, dendritic cell, peroxisome proliferator activated receptor (PPAR) γ, atherosclerosis |
url |
http://www.sciencedirect.com/science/article/pii/S1347861319305183 |
work_keys_str_mv |
AT hongyingliu danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT shijunwang danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT aijunsun danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT donghuang danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT weiwang danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT chunyuzhang danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT dazhuoshi danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT kejichen danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT yunzengzou danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway AT junboge danhonginhibitsoxidizedlowdensitylipoproteininducedimmunematurationofdentriticcellsviaaperoxisomeproliferatoractivatedreceptorgmediatedpathway |
_version_ |
1724825695187632128 |