Investigation of the Antiproliferative Properties of Natural Sesquiterpenes from Artemisia asiatica and Onopordum acanthium on HL-60 Cells in Vitro
Plants and plant extracts play a crucial role in the research into novel antineoplastic agents. Four sesquiterpene lactones, artecanin (1), 3β-chloro-4α,10α-dihydroxy-1α,2α-epoxy-5α,7αH-guaia-11(13)-en-12,6α-olide (2), iso-seco-tanapartholide 3-O-methyl ether (3) and 4β,15-dihydro-3-dehydrozaluzanin...
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doaj-1859aaa4807343269bfc6ffaff32d73d2020-11-25T02:27:41ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-02-011728310.3390/ijms17020083ijms17020083Investigation of the Antiproliferative Properties of Natural Sesquiterpenes from Artemisia asiatica and Onopordum acanthium on HL-60 Cells in VitroJudit Molnár0Gábor J. Szebeni1Boglárka Csupor-Löffler2Zsuzsanna Hajdú3Thomas Szekeres4Philipp Saiko5Imre Ocsovszki6László G. Puskás7Judit Hohmann8István Zupkó9Department of Pharmacodynamics and Biopharmacy, University of Szeged, H-6720 Szeged, HungaryAVIDIN Ltd., H-6726 Szeged, HungaryDepartment of Pharmacognosy, University of Szeged, H-6720 Szeged, HungaryDepartment of Pharmacognosy, University of Szeged, H-6720 Szeged, HungaryDepartment of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, A-1090 Vienna, AustriaDepartment of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, A-1090 Vienna, AustriaDepartment of Biochemistry, University of Szeged, H-6720 Szeged, HungaryAVIDIN Ltd., H-6726 Szeged, HungaryDepartment of Pharmacognosy, University of Szeged, H-6720 Szeged, HungaryDepartment of Pharmacodynamics and Biopharmacy, University of Szeged, H-6720 Szeged, HungaryPlants and plant extracts play a crucial role in the research into novel antineoplastic agents. Four sesquiterpene lactones, artecanin (1), 3β-chloro-4α,10α-dihydroxy-1α,2α-epoxy-5α,7αH-guaia-11(13)-en-12,6α-olide (2), iso-seco-tanapartholide 3-O-methyl ether (3) and 4β,15-dihydro-3-dehydrozaluzanin C (4), were isolated from two traditionally used Asteraceae species (Onopordum acanthium and Artemisia asiatica). When tested for antiproliferative action on HL-60 leukemia cells, these compounds exhibited reasonable IC50 values in the range 3.6–13.5 μM. Treatment with the tested compounds resulted in a cell cycle disturbance characterized by increases in the G1 and G2/M populations, while there was a decrease in the S phase. Additionally, 1–3 elicited increases in the hypodiploid (subG1) population. The compounds elicited concentration-dependent chromatin condensation and disruption of the membrane integrity, as revealed by Hoechst 33258–propidium staining. Treatment for 24 h resulted in significant increases in activity of caspases-3 and -9, indicating that the tested sesquiterpenes induced the mitochondrial pathway of apoptosis. The proapoptotic properties of the sesquiterpene lactones were additionally demonstrated withannexin V staining. Compounds 1 and 2 increased the Bax/Bcl-2 expression and decreased the expressions of CDK1 and cyclin B2, as determined at the mRNA level by means of RT-PCR. These experimental results indicate that sesquiterpene lactones may be regarded as potential starting structures for the development of novel anticancer agents.http://www.mdpi.com/1422-0067/17/2/83sesquiterpenesleukemia cellscell cycleapoptosis |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Judit Molnár Gábor J. Szebeni Boglárka Csupor-Löffler Zsuzsanna Hajdú Thomas Szekeres Philipp Saiko Imre Ocsovszki László G. Puskás Judit Hohmann István Zupkó |
spellingShingle |
Judit Molnár Gábor J. Szebeni Boglárka Csupor-Löffler Zsuzsanna Hajdú Thomas Szekeres Philipp Saiko Imre Ocsovszki László G. Puskás Judit Hohmann István Zupkó Investigation of the Antiproliferative Properties of Natural Sesquiterpenes from Artemisia asiatica and Onopordum acanthium on HL-60 Cells in Vitro International Journal of Molecular Sciences sesquiterpenes leukemia cells cell cycle apoptosis |
author_facet |
Judit Molnár Gábor J. Szebeni Boglárka Csupor-Löffler Zsuzsanna Hajdú Thomas Szekeres Philipp Saiko Imre Ocsovszki László G. Puskás Judit Hohmann István Zupkó |
author_sort |
Judit Molnár |
title |
Investigation of the Antiproliferative Properties of Natural Sesquiterpenes from Artemisia asiatica and Onopordum acanthium on HL-60 Cells in Vitro |
title_short |
Investigation of the Antiproliferative Properties of Natural Sesquiterpenes from Artemisia asiatica and Onopordum acanthium on HL-60 Cells in Vitro |
title_full |
Investigation of the Antiproliferative Properties of Natural Sesquiterpenes from Artemisia asiatica and Onopordum acanthium on HL-60 Cells in Vitro |
title_fullStr |
Investigation of the Antiproliferative Properties of Natural Sesquiterpenes from Artemisia asiatica and Onopordum acanthium on HL-60 Cells in Vitro |
title_full_unstemmed |
Investigation of the Antiproliferative Properties of Natural Sesquiterpenes from Artemisia asiatica and Onopordum acanthium on HL-60 Cells in Vitro |
title_sort |
investigation of the antiproliferative properties of natural sesquiterpenes from artemisia asiatica and onopordum acanthium on hl-60 cells in vitro |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2016-02-01 |
description |
Plants and plant extracts play a crucial role in the research into novel antineoplastic agents. Four sesquiterpene lactones, artecanin (1), 3β-chloro-4α,10α-dihydroxy-1α,2α-epoxy-5α,7αH-guaia-11(13)-en-12,6α-olide (2), iso-seco-tanapartholide 3-O-methyl ether (3) and 4β,15-dihydro-3-dehydrozaluzanin C (4), were isolated from two traditionally used Asteraceae species (Onopordum acanthium and Artemisia asiatica). When tested for antiproliferative action on HL-60 leukemia cells, these compounds exhibited reasonable IC50 values in the range 3.6–13.5 μM. Treatment with the tested compounds resulted in a cell cycle disturbance characterized by increases in the G1 and G2/M populations, while there was a decrease in the S phase. Additionally, 1–3 elicited increases in the hypodiploid (subG1) population. The compounds elicited concentration-dependent chromatin condensation and disruption of the membrane integrity, as revealed by Hoechst 33258–propidium staining. Treatment for 24 h resulted in significant increases in activity of caspases-3 and -9, indicating that the tested sesquiterpenes induced the mitochondrial pathway of apoptosis. The proapoptotic properties of the sesquiterpene lactones were additionally demonstrated withannexin V staining. Compounds 1 and 2 increased the Bax/Bcl-2 expression and decreased the expressions of CDK1 and cyclin B2, as determined at the mRNA level by means of RT-PCR. These experimental results indicate that sesquiterpene lactones may be regarded as potential starting structures for the development of novel anticancer agents. |
topic |
sesquiterpenes leukemia cells cell cycle apoptosis |
url |
http://www.mdpi.com/1422-0067/17/2/83 |
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