Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites
In this work, a novel electrochemical aptasensor was developed for sensitive and selective detection of myoglobin based on meso-tetra (4-carboxyphenyl) porphyrin-functionalized graphene-conjugated gold nanoparticles (TCPP–Gr/AuNPs). Due to its good electric conductivity, large specific surface area,...
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2016-10-01
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doaj-1863ad6050c24b5cb29cd8a6bb9e37612020-11-25T00:20:25ZengMDPI AGSensors1424-82202016-10-011611180310.3390/s16111803s16111803Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold NanocompositesGuojuan Zhang0Zhiguang Liu1Li Wang2Yujing Guo3Institute of Environmental Science, Shanxi University, Taiyuan 030006, ChinaInstitute of Environmental Science, Shanxi University, Taiyuan 030006, ChinaInstitute of Environmental Science, Shanxi University, Taiyuan 030006, ChinaInstitute of Environmental Science, Shanxi University, Taiyuan 030006, ChinaIn this work, a novel electrochemical aptasensor was developed for sensitive and selective detection of myoglobin based on meso-tetra (4-carboxyphenyl) porphyrin-functionalized graphene-conjugated gold nanoparticles (TCPP–Gr/AuNPs). Due to its good electric conductivity, large specific surface area, and excellent mechanical properties, TCPP–Gr/AuNPs can act as an enhanced material for the electrochemical detection of myoglobin. Meanwhile, it provides an effective matrix for immobilizing myoglobin-binding aptamer (MbBA). The electrochemical aptasensor has a sensitive response to myoglobin in a linear range from 2.0 × 10−11 M to 7.7 × 10−7 M with a detection limit of 6.7 × 10−12 M (S/N = 3). Furthermore, the method has the merits of high sensitivity, low price, and high specificity. Our work will supply new horizons for the diagnostic applications of graphene-based materials in biomedicine and biosensors.http://www.mdpi.com/1424-8220/16/11/1803grapheneTCPPAuNPsmyoglobin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guojuan Zhang Zhiguang Liu Li Wang Yujing Guo |
spellingShingle |
Guojuan Zhang Zhiguang Liu Li Wang Yujing Guo Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites Sensors graphene TCPP AuNPs myoglobin |
author_facet |
Guojuan Zhang Zhiguang Liu Li Wang Yujing Guo |
author_sort |
Guojuan Zhang |
title |
Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites |
title_short |
Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites |
title_full |
Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites |
title_fullStr |
Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites |
title_full_unstemmed |
Electrochemical Aptasensor for Myoglobin-Specific Recognition Based on Porphyrin Functionalized Graphene-Conjugated Gold Nanocomposites |
title_sort |
electrochemical aptasensor for myoglobin-specific recognition based on porphyrin functionalized graphene-conjugated gold nanocomposites |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2016-10-01 |
description |
In this work, a novel electrochemical aptasensor was developed for sensitive and selective detection of myoglobin based on meso-tetra (4-carboxyphenyl) porphyrin-functionalized graphene-conjugated gold nanoparticles (TCPP–Gr/AuNPs). Due to its good electric conductivity, large specific surface area, and excellent mechanical properties, TCPP–Gr/AuNPs can act as an enhanced material for the electrochemical detection of myoglobin. Meanwhile, it provides an effective matrix for immobilizing myoglobin-binding aptamer (MbBA). The electrochemical aptasensor has a sensitive response to myoglobin in a linear range from 2.0 × 10−11 M to 7.7 × 10−7 M with a detection limit of 6.7 × 10−12 M (S/N = 3). Furthermore, the method has the merits of high sensitivity, low price, and high specificity. Our work will supply new horizons for the diagnostic applications of graphene-based materials in biomedicine and biosensors. |
topic |
graphene TCPP AuNPs myoglobin |
url |
http://www.mdpi.com/1424-8220/16/11/1803 |
work_keys_str_mv |
AT guojuanzhang electrochemicalaptasensorformyoglobinspecificrecognitionbasedonporphyrinfunctionalizedgrapheneconjugatedgoldnanocomposites AT zhiguangliu electrochemicalaptasensorformyoglobinspecificrecognitionbasedonporphyrinfunctionalizedgrapheneconjugatedgoldnanocomposites AT liwang electrochemicalaptasensorformyoglobinspecificrecognitionbasedonporphyrinfunctionalizedgrapheneconjugatedgoldnanocomposites AT yujingguo electrochemicalaptasensorformyoglobinspecificrecognitionbasedonporphyrinfunctionalizedgrapheneconjugatedgoldnanocomposites |
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