In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green
Conventional X-ray-based cystography uses radio-opaque materials, but this method uses harmful ionizing radiation and is not sensitive. In this study, we demonstrate nonionizing and noninvasive photoacoustic (PA) and fluorescence (FL) cystography using clinically relevant indocyanine green (ICG) in...
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doaj-1869ae7587ac4f65b61fb743fabac5af2020-11-24T21:47:20ZengMDPI AGSensors1424-82202014-10-011410196601966810.3390/s141019660s141019660In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine GreenSungjo Park0Jeesu Kim1Mansik Jeon2Jaewon Song3Chulhong Kim4School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370, Sankyuk-dong, Buk-gu, Daegu 702-701, KoreaDepartments of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, KoreaDepartments of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, KoreaSchool of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370, Sankyuk-dong, Buk-gu, Daegu 702-701, KoreaDepartments of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, KoreaConventional X-ray-based cystography uses radio-opaque materials, but this method uses harmful ionizing radiation and is not sensitive. In this study, we demonstrate nonionizing and noninvasive photoacoustic (PA) and fluorescence (FL) cystography using clinically relevant indocyanine green (ICG) in vivo. After transurethral injection of ICG into rats through a catheter, their bladders were photoacoustically and fluorescently visualized. A deeply positioned bladder below the skin surface (i.e., ~1.5–5 mm) was clearly visible in the PA and FL image using a laser pulse energy of less than 2 mJ/cm2 (1/15 of the safety limit). Then, the in vivo imaging results were validated through in situ studies. Our results suggest that dual modal cystography can provide a nonionizing and noninvasive imaging tool for bladder mapping.http://www.mdpi.com/1424-8220/14/10/19660photoacoustic tomographyfluorescence imagecystographyindocyanine green |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sungjo Park Jeesu Kim Mansik Jeon Jaewon Song Chulhong Kim |
spellingShingle |
Sungjo Park Jeesu Kim Mansik Jeon Jaewon Song Chulhong Kim In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green Sensors photoacoustic tomography fluorescence image cystography indocyanine green |
author_facet |
Sungjo Park Jeesu Kim Mansik Jeon Jaewon Song Chulhong Kim |
author_sort |
Sungjo Park |
title |
In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green |
title_short |
In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green |
title_full |
In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green |
title_fullStr |
In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green |
title_full_unstemmed |
In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green |
title_sort |
in vivo photoacoustic and fluorescence cystography using clinically relevant dual modal indocyanine green |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2014-10-01 |
description |
Conventional X-ray-based cystography uses radio-opaque materials, but this method uses harmful ionizing radiation and is not sensitive. In this study, we demonstrate nonionizing and noninvasive photoacoustic (PA) and fluorescence (FL) cystography using clinically relevant indocyanine green (ICG) in vivo. After transurethral injection of ICG into rats through a catheter, their bladders were photoacoustically and fluorescently visualized. A deeply positioned bladder below the skin surface (i.e., ~1.5–5 mm) was clearly visible in the PA and FL image using a laser pulse energy of less than 2 mJ/cm2 (1/15 of the safety limit). Then, the in vivo imaging results were validated through in situ studies. Our results suggest that dual modal cystography can provide a nonionizing and noninvasive imaging tool for bladder mapping. |
topic |
photoacoustic tomography fluorescence image cystography indocyanine green |
url |
http://www.mdpi.com/1424-8220/14/10/19660 |
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