In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green

Conventional X-ray-based cystography uses radio-opaque materials, but this method uses harmful ionizing radiation and is not sensitive. In this study, we demonstrate nonionizing and noninvasive photoacoustic (PA) and fluorescence (FL) cystography using clinically relevant indocyanine green (ICG) in...

Full description

Bibliographic Details
Main Authors: Sungjo Park, Jeesu Kim, Mansik Jeon, Jaewon Song, Chulhong Kim
Format: Article
Language:English
Published: MDPI AG 2014-10-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/14/10/19660
id doaj-1869ae7587ac4f65b61fb743fabac5af
record_format Article
spelling doaj-1869ae7587ac4f65b61fb743fabac5af2020-11-24T21:47:20ZengMDPI AGSensors1424-82202014-10-011410196601966810.3390/s141019660s141019660In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine GreenSungjo Park0Jeesu Kim1Mansik Jeon2Jaewon Song3Chulhong Kim4School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370, Sankyuk-dong, Buk-gu, Daegu 702-701, KoreaDepartments of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, KoreaDepartments of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, KoreaSchool of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370, Sankyuk-dong, Buk-gu, Daegu 702-701, KoreaDepartments of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, KoreaConventional X-ray-based cystography uses radio-opaque materials, but this method uses harmful ionizing radiation and is not sensitive. In this study, we demonstrate nonionizing and noninvasive photoacoustic (PA) and fluorescence (FL) cystography using clinically relevant indocyanine green (ICG) in vivo. After transurethral injection of ICG into rats through a catheter, their bladders were photoacoustically and fluorescently visualized. A deeply positioned bladder below the skin surface (i.e., ~1.5–5 mm) was clearly visible in the PA and FL image using a laser pulse energy of less than 2 mJ/cm2 (1/15 of the safety limit). Then, the in vivo imaging results were validated through in situ studies. Our results suggest that dual modal cystography can provide a nonionizing and noninvasive imaging tool for bladder mapping.http://www.mdpi.com/1424-8220/14/10/19660photoacoustic tomographyfluorescence imagecystographyindocyanine green
collection DOAJ
language English
format Article
sources DOAJ
author Sungjo Park
Jeesu Kim
Mansik Jeon
Jaewon Song
Chulhong Kim
spellingShingle Sungjo Park
Jeesu Kim
Mansik Jeon
Jaewon Song
Chulhong Kim
In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green
Sensors
photoacoustic tomography
fluorescence image
cystography
indocyanine green
author_facet Sungjo Park
Jeesu Kim
Mansik Jeon
Jaewon Song
Chulhong Kim
author_sort Sungjo Park
title In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green
title_short In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green
title_full In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green
title_fullStr In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green
title_full_unstemmed In Vivo Photoacoustic and Fluorescence Cystography Using Clinically Relevant Dual Modal Indocyanine Green
title_sort in vivo photoacoustic and fluorescence cystography using clinically relevant dual modal indocyanine green
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2014-10-01
description Conventional X-ray-based cystography uses radio-opaque materials, but this method uses harmful ionizing radiation and is not sensitive. In this study, we demonstrate nonionizing and noninvasive photoacoustic (PA) and fluorescence (FL) cystography using clinically relevant indocyanine green (ICG) in vivo. After transurethral injection of ICG into rats through a catheter, their bladders were photoacoustically and fluorescently visualized. A deeply positioned bladder below the skin surface (i.e., ~1.5–5 mm) was clearly visible in the PA and FL image using a laser pulse energy of less than 2 mJ/cm2 (1/15 of the safety limit). Then, the in vivo imaging results were validated through in situ studies. Our results suggest that dual modal cystography can provide a nonionizing and noninvasive imaging tool for bladder mapping.
topic photoacoustic tomography
fluorescence image
cystography
indocyanine green
url http://www.mdpi.com/1424-8220/14/10/19660
work_keys_str_mv AT sungjopark invivophotoacousticandfluorescencecystographyusingclinicallyrelevantdualmodalindocyaninegreen
AT jeesukim invivophotoacousticandfluorescencecystographyusingclinicallyrelevantdualmodalindocyaninegreen
AT mansikjeon invivophotoacousticandfluorescencecystographyusingclinicallyrelevantdualmodalindocyaninegreen
AT jaewonsong invivophotoacousticandfluorescencecystographyusingclinicallyrelevantdualmodalindocyaninegreen
AT chulhongkim invivophotoacousticandfluorescencecystographyusingclinicallyrelevantdualmodalindocyaninegreen
_version_ 1725897649694965760