Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan.
BACKGROUND:Mosquitoes feed on plant-derived fluids such as nectar and sap and are exposed to bioactive molecules found in this dietary source. However, the role of such molecules on mosquito vectorial capacity is unknown. Weather has been recognized as a major determinant of the spread of dengue, an...
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doaj-186c3cd4714f4e11af3339d8c255de502020-11-24T21:58:51ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352016-10-011010e000503410.1371/journal.pntd.0005034Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan.Rodrigo Dutra NunesGuilherme Ventura-MartinsDébora Monteiro MorettiPriscilla Medeiros-CastroCarlucio Rocha-SantosCarlos Renato de Oliveira Daumas-FilhoPaula Rego Barros Bittencourt-CunhaKarina Martins-CardosoCecília Oliveira CudischevitchRubem Figueiredo Sadok Menna-BarretoJosé Henrique Maia OliveiraDesiely Silva GusmãoFrancisco José Alves LemosDaniela Sales AlvianoPedro Lagerblad OliveiraCarl LowenbergerDavid MajerowiczRicardo Melo OliveiraRafael Dias MesquitaGeorgia Correa AtellaMário Alberto Cardoso Silva-NetoBACKGROUND:Mosquitoes feed on plant-derived fluids such as nectar and sap and are exposed to bioactive molecules found in this dietary source. However, the role of such molecules on mosquito vectorial capacity is unknown. Weather has been recognized as a major determinant of the spread of dengue, and plants under abiotic stress increase their production of polyphenols. RESULTS:Here, we show that including polyphenols in mosquito meals promoted the activation of AMP-dependent protein kinase (AMPK). AMPK positively regulated midgut autophagy leading to a decrease in bacterial proliferation and an increase in vector lifespan. Suppression of AMPK activity resulted in a 6-fold increase in midgut microbiota. Similarly, inhibition of polyphenol-induced autophagy induced an 8-fold increase in bacterial proliferation. Mosquitoes maintained on the polyphenol diet were readily infected by dengue virus. CONCLUSION:The present findings uncover a new direct route by which exacerbation of autophagy through activation of the AMPK pathway leads to a more efficient control of mosquito midgut microbiota and increases the average mosquito lifespan. Our results suggest for the first time that the polyphenol content and availability of the surrounding vegetation may increase the population of mosquitoes prone to infection with arboviruses.http://europepmc.org/articles/PMC5061323?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rodrigo Dutra Nunes Guilherme Ventura-Martins Débora Monteiro Moretti Priscilla Medeiros-Castro Carlucio Rocha-Santos Carlos Renato de Oliveira Daumas-Filho Paula Rego Barros Bittencourt-Cunha Karina Martins-Cardoso Cecília Oliveira Cudischevitch Rubem Figueiredo Sadok Menna-Barreto José Henrique Maia Oliveira Desiely Silva Gusmão Francisco José Alves Lemos Daniela Sales Alviano Pedro Lagerblad Oliveira Carl Lowenberger David Majerowicz Ricardo Melo Oliveira Rafael Dias Mesquita Georgia Correa Atella Mário Alberto Cardoso Silva-Neto |
spellingShingle |
Rodrigo Dutra Nunes Guilherme Ventura-Martins Débora Monteiro Moretti Priscilla Medeiros-Castro Carlucio Rocha-Santos Carlos Renato de Oliveira Daumas-Filho Paula Rego Barros Bittencourt-Cunha Karina Martins-Cardoso Cecília Oliveira Cudischevitch Rubem Figueiredo Sadok Menna-Barreto José Henrique Maia Oliveira Desiely Silva Gusmão Francisco José Alves Lemos Daniela Sales Alviano Pedro Lagerblad Oliveira Carl Lowenberger David Majerowicz Ricardo Melo Oliveira Rafael Dias Mesquita Georgia Correa Atella Mário Alberto Cardoso Silva-Neto Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan. PLoS Neglected Tropical Diseases |
author_facet |
Rodrigo Dutra Nunes Guilherme Ventura-Martins Débora Monteiro Moretti Priscilla Medeiros-Castro Carlucio Rocha-Santos Carlos Renato de Oliveira Daumas-Filho Paula Rego Barros Bittencourt-Cunha Karina Martins-Cardoso Cecília Oliveira Cudischevitch Rubem Figueiredo Sadok Menna-Barreto José Henrique Maia Oliveira Desiely Silva Gusmão Francisco José Alves Lemos Daniela Sales Alviano Pedro Lagerblad Oliveira Carl Lowenberger David Majerowicz Ricardo Melo Oliveira Rafael Dias Mesquita Georgia Correa Atella Mário Alberto Cardoso Silva-Neto |
author_sort |
Rodrigo Dutra Nunes |
title |
Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan. |
title_short |
Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan. |
title_full |
Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan. |
title_fullStr |
Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan. |
title_full_unstemmed |
Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan. |
title_sort |
polyphenol-rich diets exacerbate ampk-mediated autophagy, decreasing proliferation of mosquito midgut microbiota, and extending vector lifespan. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Neglected Tropical Diseases |
issn |
1935-2727 1935-2735 |
publishDate |
2016-10-01 |
description |
BACKGROUND:Mosquitoes feed on plant-derived fluids such as nectar and sap and are exposed to bioactive molecules found in this dietary source. However, the role of such molecules on mosquito vectorial capacity is unknown. Weather has been recognized as a major determinant of the spread of dengue, and plants under abiotic stress increase their production of polyphenols. RESULTS:Here, we show that including polyphenols in mosquito meals promoted the activation of AMP-dependent protein kinase (AMPK). AMPK positively regulated midgut autophagy leading to a decrease in bacterial proliferation and an increase in vector lifespan. Suppression of AMPK activity resulted in a 6-fold increase in midgut microbiota. Similarly, inhibition of polyphenol-induced autophagy induced an 8-fold increase in bacterial proliferation. Mosquitoes maintained on the polyphenol diet were readily infected by dengue virus. CONCLUSION:The present findings uncover a new direct route by which exacerbation of autophagy through activation of the AMPK pathway leads to a more efficient control of mosquito midgut microbiota and increases the average mosquito lifespan. Our results suggest for the first time that the polyphenol content and availability of the surrounding vegetation may increase the population of mosquitoes prone to infection with arboviruses. |
url |
http://europepmc.org/articles/PMC5061323?pdf=render |
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