Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy
Super-resolution microscopy offers a non-invasive and real-time tool for probing the subcellular structures and activities on nanometer precision. Exploring adequate luminescent probes is a great concern for acquiring higher-resolution image. Benefiting from the atomic-like transitions among real en...
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doaj-186d06963ac84c12b15942762297eda32021-01-15T04:36:43ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-01-01810.3389/fchem.2020.619377619377Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution MicroscopyHao Dong0Ling-Dong Sun1Chun-Hua Yan2Chun-Hua Yan3Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, ChinaBeijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, ChinaBeijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, ChinaCollege of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, ChinaSuper-resolution microscopy offers a non-invasive and real-time tool for probing the subcellular structures and activities on nanometer precision. Exploring adequate luminescent probes is a great concern for acquiring higher-resolution image. Benefiting from the atomic-like transitions among real energy levels, lanthanide-doped upconversion nanoparticles are featured by unique optical properties including excellent photostability, large anti-Stokes shifts, multicolor narrowband emissions, tunable emission lifetimes, etc. The past few years have witnessed the development of upconversion nanoparticles as probes for super-resolution imaging studies. To date, the optimal resolution reached 28 nm (λ/36) for single nanoparticles and 82 nm (λ/12) for cytoskeleton structures with upconversion nanoparticles. Compared with conventional probes such as organic dyes and quantum dots, upconversion nanoparticle-related super-resolution microscopy is still in the preliminary stage, and both opportunities and challenges exist. In this perspective article, we summarized the recent advances of upconversion nanoparticles for super-resolution microscopy and projected the future directions of this emerging field. This perspective article should be enlightening for designing efficient upconversion nanoprobes for super-resolution imaging and promote the development of upconversion nanoprobes for cell biology applications.https://www.frontiersin.org/articles/10.3389/fchem.2020.619377/fullupconversion nanoparticlesuper-resolution microscopylanthanideSTEDmultiphoton imaging |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hao Dong Ling-Dong Sun Chun-Hua Yan Chun-Hua Yan |
spellingShingle |
Hao Dong Ling-Dong Sun Chun-Hua Yan Chun-Hua Yan Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy Frontiers in Chemistry upconversion nanoparticle super-resolution microscopy lanthanide STED multiphoton imaging |
author_facet |
Hao Dong Ling-Dong Sun Chun-Hua Yan Chun-Hua Yan |
author_sort |
Hao Dong |
title |
Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy |
title_short |
Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy |
title_full |
Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy |
title_fullStr |
Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy |
title_full_unstemmed |
Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy |
title_sort |
lanthanide-doped upconversion nanoparticles for super-resolution microscopy |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Chemistry |
issn |
2296-2646 |
publishDate |
2021-01-01 |
description |
Super-resolution microscopy offers a non-invasive and real-time tool for probing the subcellular structures and activities on nanometer precision. Exploring adequate luminescent probes is a great concern for acquiring higher-resolution image. Benefiting from the atomic-like transitions among real energy levels, lanthanide-doped upconversion nanoparticles are featured by unique optical properties including excellent photostability, large anti-Stokes shifts, multicolor narrowband emissions, tunable emission lifetimes, etc. The past few years have witnessed the development of upconversion nanoparticles as probes for super-resolution imaging studies. To date, the optimal resolution reached 28 nm (λ/36) for single nanoparticles and 82 nm (λ/12) for cytoskeleton structures with upconversion nanoparticles. Compared with conventional probes such as organic dyes and quantum dots, upconversion nanoparticle-related super-resolution microscopy is still in the preliminary stage, and both opportunities and challenges exist. In this perspective article, we summarized the recent advances of upconversion nanoparticles for super-resolution microscopy and projected the future directions of this emerging field. This perspective article should be enlightening for designing efficient upconversion nanoprobes for super-resolution imaging and promote the development of upconversion nanoprobes for cell biology applications. |
topic |
upconversion nanoparticle super-resolution microscopy lanthanide STED multiphoton imaging |
url |
https://www.frontiersin.org/articles/10.3389/fchem.2020.619377/full |
work_keys_str_mv |
AT haodong lanthanidedopedupconversionnanoparticlesforsuperresolutionmicroscopy AT lingdongsun lanthanidedopedupconversionnanoparticlesforsuperresolutionmicroscopy AT chunhuayan lanthanidedopedupconversionnanoparticlesforsuperresolutionmicroscopy AT chunhuayan lanthanidedopedupconversionnanoparticlesforsuperresolutionmicroscopy |
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