Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota
Water buffaloes mastitis represents a major issue in terms of animal health, cost of therapy, premature culling and decreased milk yeld. The emergence of antibiotic resistance has led to investigate strategies in order to avoid or minimize the antibiotic use, especially during subclinical mastitis d...
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Format: | Article |
Language: | English |
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Università degli Studi di Milano
2018-06-01
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Series: | International Journal of Health, Animal Science and Food Safety |
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Online Access: | https://riviste.unimi.it/index.php/haf/article/view/10018 |
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doaj-187ceaa0c60c44f8b3ff3e5fe42fac99 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carlotta Catozzi Anna Cuscó Martí Cristina Lecchi Valentina Zamarian Joaquim Viñes Pujol Sara D'andreano Alessandra Martucciello Giovanna Cappelli Carlo Grassi Cinzia Marianelli Luisa D'Angelo Esterina De Carlo Domenico Vecchio Armand Sanchez Bonastre Olga Francino Fabrizio Caciliani |
spellingShingle |
Carlotta Catozzi Anna Cuscó Martí Cristina Lecchi Valentina Zamarian Joaquim Viñes Pujol Sara D'andreano Alessandra Martucciello Giovanna Cappelli Carlo Grassi Cinzia Marianelli Luisa D'Angelo Esterina De Carlo Domenico Vecchio Armand Sanchez Bonastre Olga Francino Fabrizio Caciliani Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota International Journal of Health, Animal Science and Food Safety Water buffalo, Milk microbiota, Subclinical mastitis |
author_facet |
Carlotta Catozzi Anna Cuscó Martí Cristina Lecchi Valentina Zamarian Joaquim Viñes Pujol Sara D'andreano Alessandra Martucciello Giovanna Cappelli Carlo Grassi Cinzia Marianelli Luisa D'Angelo Esterina De Carlo Domenico Vecchio Armand Sanchez Bonastre Olga Francino Fabrizio Caciliani |
author_sort |
Carlotta Catozzi |
title |
Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota |
title_short |
Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota |
title_full |
Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota |
title_fullStr |
Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota |
title_full_unstemmed |
Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota |
title_sort |
effect of inactivated cultures of lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota |
publisher |
Università degli Studi di Milano |
series |
International Journal of Health, Animal Science and Food Safety |
issn |
2283-3927 |
publishDate |
2018-06-01 |
description |
Water buffaloes mastitis represents a major issue in terms of animal health, cost of therapy, premature culling and decreased milk yeld. The emergence of antibiotic resistance has led to investigate strategies in order to avoid or minimize the antibiotic use, especially during subclinical mastitis disease (SM) (1). Lactobacillus rhamnosus is part of the normal gut microflora, having meanwhile an immunostimulatory activity (2). The aim of this study was to investigate the change of milk microbiota after the theraputic treatment with inactivated cultures of Lactobacillus rhamnosus of mammary gland quarters affected by subclinical mastitis. A number of 43 quarters were included in the study, and were treated with antibiotics, Lactobacillus rhamnosus and PBS as negative control. Samples were collected at two time points, T0 and T5 (days) and V4 region of 16S rRNA gene was amplified by PCR and sequenced using Ion Torrent Personal Genome Machine. The microbiota structure of SM quarters showed no major changes after PBS treatment, while differed after antibiotic treatment where Staphylococcus decreased its relative abundance from 41% at T0 to 3% at T5. Lactobacillus rhamnosus induced a less dramatic change in milk microbiota, although the relative abundance of some genera were found to be modifidied, among which an increase of Pseudomonas from 1.5% at T0 up to 4% at T5. No differences were present between the microbiota structure of quarters treated with Lactobacillus rhamnosus and PBS. This study allowed to characterize the changes of microbiota in milk from animals with Lactobacillus rhamnosus and antibiotics. While changes in milk microbiota after antibiotic treatment were evident, changes after Lactobacillus rhamnosus were more limited. Further investigations are needed to evaluate alternative strategies to mastitis treatment. |
topic |
Water buffalo, Milk microbiota, Subclinical mastitis |
url |
https://riviste.unimi.it/index.php/haf/article/view/10018 |
work_keys_str_mv |
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doaj-187ceaa0c60c44f8b3ff3e5fe42fac992020-11-25T03:53:51ZengUniversità degli Studi di MilanoInternational Journal of Health, Animal Science and Food Safety2283-39272018-06-0151s10.13130/2283-3927/100188731Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiotaCarlotta Catozzi0Anna Cuscó Martí1Cristina Lecchi2Valentina Zamarian3Joaquim Viñes Pujol4Sara D'andreano5Alessandra Martucciello6Giovanna Cappelli7Carlo GrassiCinzia Marianelli8Luisa D'Angelo9Esterina De Carlo10Domenico Vecchio11Armand Sanchez Bonastre12Olga Francino13Fabrizio Caciliani14Veterinary Medicine Department, Università degli Studi di Milano, Via Celoria 10, Milano, Italy.Vetgenomics. Ed Eureka. PRUAB. Campus UAB, Bellaterra, Barcelona, Spain.Veterinary Medicine Department, Università degli Studi di Milano, Via Celoria 10, Milano, Italy.Veterinary Medicine Department, Università degli Studi di Milano, Via Celoria 10, Milano, Italy.Vetgenomics. Ed Eureka. PRUAB. Campus UAB, Bellaterra, Barcelona, Spain.Vetgenomics. Ed Eureka. PRUAB. Campus UAB, Bellaterra, Barcelona, Spain.Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.Unit of Prophylaxis and Control of Bacterial Zoonoses. Department Of Veterinary Public Health and Food Safety. Istituto Superiore di Sanità. Viale Regina Elena 299, Rome, Italy.Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.Molecular Genetics Veterinary Service (SVGM), Veterinary School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, SpainMolecular Genetics Veterinary Service (SVGM), Veterinary School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, SpainVeterinary Medicine Department, Università degli Studi di Milano, Via Celoria 10, Milano, Italy.Water buffaloes mastitis represents a major issue in terms of animal health, cost of therapy, premature culling and decreased milk yeld. The emergence of antibiotic resistance has led to investigate strategies in order to avoid or minimize the antibiotic use, especially during subclinical mastitis disease (SM) (1). Lactobacillus rhamnosus is part of the normal gut microflora, having meanwhile an immunostimulatory activity (2). The aim of this study was to investigate the change of milk microbiota after the theraputic treatment with inactivated cultures of Lactobacillus rhamnosus of mammary gland quarters affected by subclinical mastitis. A number of 43 quarters were included in the study, and were treated with antibiotics, Lactobacillus rhamnosus and PBS as negative control. Samples were collected at two time points, T0 and T5 (days) and V4 region of 16S rRNA gene was amplified by PCR and sequenced using Ion Torrent Personal Genome Machine. The microbiota structure of SM quarters showed no major changes after PBS treatment, while differed after antibiotic treatment where Staphylococcus decreased its relative abundance from 41% at T0 to 3% at T5. Lactobacillus rhamnosus induced a less dramatic change in milk microbiota, although the relative abundance of some genera were found to be modifidied, among which an increase of Pseudomonas from 1.5% at T0 up to 4% at T5. No differences were present between the microbiota structure of quarters treated with Lactobacillus rhamnosus and PBS. This study allowed to characterize the changes of microbiota in milk from animals with Lactobacillus rhamnosus and antibiotics. While changes in milk microbiota after antibiotic treatment were evident, changes after Lactobacillus rhamnosus were more limited. Further investigations are needed to evaluate alternative strategies to mastitis treatment.https://riviste.unimi.it/index.php/haf/article/view/10018Water buffalo, Milk microbiota, Subclinical mastitis |