Voltage-gated potassium channels are involved in oxymatrine-regulated islet function in rat islet β cells and INS-1 cells

• Main Authors: Jingying Gao, Lixia xia, Yuanyuan Wei
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2021-04-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: apoptosis diabetes mellitus insulin secretion oxymatrine potassium channel voltage; gated
• Online Access: https://ijbms.mums.ac.ir/article_17755_742e709253b90b76eb5cae310ff59e81.pdf
• ISSN: 2008-3866; 2008-3874

Objective(s): Oxymatrine can regulate glucose metabolism. But the underlying mechanisms remain unclear. We investigated the relationship of oxymatrine and voltage-gated potassium (Kv) channel in rat islet β cells and INS-1 cells.Materials and Methods: Insulin secretion and Kv channel currents were tested by radioimmunoassay and patch-clamp technique, respectively. The INS-1 cell viability was detected using cell counting kit-8 experiments. Flowcytometry analysis and western blot were employed for cell apoptosis and protein levels, respectively. INS-1 cell proliferation was assessed by the 5-Ethynyl-2’- deoxyuridine method. Results: Oxymatrine potentiated insulin secretion at high glucose (p Conclusion: The results indicate that oxymatrine can stimulate insulin secretion and decrease kv channel currents in islet β cells. Besides, oxymatrine also increases cell viability, proliferation, and reduces cell apoptosis in INS-1 cells. The effects of oxymatrine are related to kv channels. This finding provides new insight into the mechanisms of oxymatrine-regulated islet function.