Anti-Inflammatory Activity of N-(3-Florophenyl)ethylcaffeamide in Mice

• Main Authors: Yueh-Hsiung Kuo, Minoru Tsuzoki, Ping-Jyun Sung, Yung-Jia Chiu, Jung-Chun Liao, Jen-Chieh Tsai, Wen-Huang Peng
• Format: Article
• Language: English
• Published: MDPI AG 2013-07-01
• Series: International Journal of Molecular Sciences
• Subjects: anti-inflammatory; caffeamide; synthesis
• Online Access: http://www.mdpi.com/1422-0067/14/8/15199

In this study, we evaluated the anti-inflammatory activity of one synthetic product, N-(3-Florophenyl)ethylcaffeamide (abbrev. FECA), by using animal model of λ-carrageenan-induced paw edema in mice. The anti-inflammatory mechanism of FECA was determined by measuring the levels of cyclooxygenase-2 (COX-2), nitric oxide (NO), tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), and malondialdehyde (MDA) in the edema paw tissue, and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GRd) in the liver. The results showed that FECA reduced the paw edema at three, four and five hours after λ-carrageenan administration. The levels of COX-2, NO, TNF-α, and MDA in the λ-carrageenan-induced edema paws were reduced and the activities of SOD, GPx, and GRd in liver tissues were raised by FECA. These results suggested that FECA possessed anti-inflammatory activities and the anti-inflammatory mechanisms might be related to the decrease of the levels of COX-2, NO, and TNF-α in inflamed tissues and the increase in the MDA level by increasing the activities of SOD, GPx, and GRd.