Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra

ABSTRACT: The electrophysiological characteristics of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra was investigated using the whole-cell patch clamp technique under the voltage-clamp mode. Nicotine (0.01 – 100 μM) induced inward currents corresponding to nicotine-i...

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Main Authors:	Hiroaki Matsubayashi, Taku Amano, Takahiro Seki, Masashi Sasa, Norio Sakai
Format:	Article
Language:	English
Published:	Elsevier 2003-01-01
Series:	Journal of Pharmacological Sciences
Online Access:	http://www.sciencedirect.com/science/article/pii/S1347861319325903

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spelling	doaj-189fbea01acf43d5b404dc906d67d4e22020-11-25T01:27:37ZengElsevierJournal of Pharmacological Sciences1347-86132003-01-01932143148Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia NigraHiroaki Matsubayashi0Taku Amano1Takahiro Seki2Masashi Sasa3Norio Sakai4Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, JapanNagisa Hospital, Osaka 573-1183, JapanDepartment of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, JapanABSTRACT: The electrophysiological characteristics of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra was investigated using the whole-cell patch clamp technique under the voltage-clamp mode. Nicotine (0.01 – 100 μM) induced inward currents corresponding to nicotine-induced depolarization with an increase in firing in a dose-dependent manner. This current was inhibited by dihydro-β-erythroidine, a selective antagonist for the α4β2 type neuronal nicotinic receptor. Nicotine directly acts on postsynaptic α4β2 type nicotinic receptors and induces inward currents, resulting in excitation of dopaminergic neurons in the substantia nigra and subsequent enhancement of dopamine release in the corpus striatum.http://www.sciencedirect.com/science/article/pii/S1347861319325903
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Hiroaki Matsubayashi Taku Amano Takahiro Seki Masashi Sasa Norio Sakai
spellingShingle	Hiroaki Matsubayashi Taku Amano Takahiro Seki Masashi Sasa Norio Sakai Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra Journal of Pharmacological Sciences
author_facet	Hiroaki Matsubayashi Taku Amano Takahiro Seki Masashi Sasa Norio Sakai
author_sort	Hiroaki Matsubayashi
title	Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra
title_short	Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra
title_full	Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra
title_fullStr	Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra
title_full_unstemmed	Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra
title_sort	electrophysiological characterization of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra
publisher	Elsevier
series	Journal of Pharmacological Sciences
issn	1347-8613
publishDate	2003-01-01
description	ABSTRACT: The electrophysiological characteristics of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra was investigated using the whole-cell patch clamp technique under the voltage-clamp mode. Nicotine (0.01 – 100 μM) induced inward currents corresponding to nicotine-induced depolarization with an increase in firing in a dose-dependent manner. This current was inhibited by dihydro-β-erythroidine, a selective antagonist for the α4β2 type neuronal nicotinic receptor. Nicotine directly acts on postsynaptic α4β2 type nicotinic receptors and induces inward currents, resulting in excitation of dopaminergic neurons in the substantia nigra and subsequent enhancement of dopamine release in the corpus striatum.
url	http://www.sciencedirect.com/science/article/pii/S1347861319325903
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Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra

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