Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra
ABSTRACT: The electrophysiological characteristics of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra was investigated using the whole-cell patch clamp technique under the voltage-clamp mode. Nicotine (0.01 – 100 μM) induced inward currents corresponding to nicotine-i...
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2003-01-01
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doaj-189fbea01acf43d5b404dc906d67d4e22020-11-25T01:27:37ZengElsevierJournal of Pharmacological Sciences1347-86132003-01-01932143148Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia NigraHiroaki Matsubayashi0Taku Amano1Takahiro Seki2Masashi Sasa3Norio Sakai4Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, JapanNagisa Hospital, Osaka 573-1183, JapanDepartment of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8551, JapanABSTRACT: The electrophysiological characteristics of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra was investigated using the whole-cell patch clamp technique under the voltage-clamp mode. Nicotine (0.01 – 100 μM) induced inward currents corresponding to nicotine-induced depolarization with an increase in firing in a dose-dependent manner. This current was inhibited by dihydro-β-erythroidine, a selective antagonist for the α4β2 type neuronal nicotinic receptor. Nicotine directly acts on postsynaptic α4β2 type nicotinic receptors and induces inward currents, resulting in excitation of dopaminergic neurons in the substantia nigra and subsequent enhancement of dopamine release in the corpus striatum.http://www.sciencedirect.com/science/article/pii/S1347861319325903 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hiroaki Matsubayashi Taku Amano Takahiro Seki Masashi Sasa Norio Sakai |
spellingShingle |
Hiroaki Matsubayashi Taku Amano Takahiro Seki Masashi Sasa Norio Sakai Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra Journal of Pharmacological Sciences |
author_facet |
Hiroaki Matsubayashi Taku Amano Takahiro Seki Masashi Sasa Norio Sakai |
author_sort |
Hiroaki Matsubayashi |
title |
Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra |
title_short |
Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra |
title_full |
Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra |
title_fullStr |
Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra |
title_full_unstemmed |
Electrophysiological Characterization of Nicotine-Induced Excitation of Dopaminergic Neurons in the Rat Substantia Nigra |
title_sort |
electrophysiological characterization of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra |
publisher |
Elsevier |
series |
Journal of Pharmacological Sciences |
issn |
1347-8613 |
publishDate |
2003-01-01 |
description |
ABSTRACT: The electrophysiological characteristics of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra was investigated using the whole-cell patch clamp technique under the voltage-clamp mode. Nicotine (0.01 – 100 μM) induced inward currents corresponding to nicotine-induced depolarization with an increase in firing in a dose-dependent manner. This current was inhibited by dihydro-β-erythroidine, a selective antagonist for the α4β2 type neuronal nicotinic receptor. Nicotine directly acts on postsynaptic α4β2 type nicotinic receptors and induces inward currents, resulting in excitation of dopaminergic neurons in the substantia nigra and subsequent enhancement of dopamine release in the corpus striatum. |
url |
http://www.sciencedirect.com/science/article/pii/S1347861319325903 |
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