Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification

Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification

To establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tiss...

Full description

Bibliographic Details
Main Authors: Masahiro Gotoh, Eri Arai, Saori Wakai-Ushijima, Nobuyoshi Hiraoka, Tomoo Kosuge, Fumie Hosoda, Tatsuhiro Shibata, Tadashi Kondo, Sana Yokoi, Issei Imoto, Johji Inazawa, Yae Kanai
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2011/780836
id doaj-18a7466ecda946f7b9366d27c1d245ad
record_format Article
spelling doaj-18a7466ecda946f7b9366d27c1d245ad2020-11-25T01:33:16ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512011-01-01201110.1155/2011/780836780836Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island AmplificationMasahiro Gotoh0Eri Arai1Saori Wakai-Ushijima2Nobuyoshi Hiraoka3Tomoo Kosuge4Fumie Hosoda5Tatsuhiro Shibata6Tadashi Kondo7Sana Yokoi8Issei Imoto9Johji Inazawa10Yae Kanai11Pathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, JapanPathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, JapanPathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, JapanPathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, JapanHepatobiliary and Pancreatic Surgery Division, National Cancer Center Hospital, Tokyo 104-0045, JapanCancer Genomics Project, National Cancer Center Research Institute, Tokyo 104-0045, JapanCancer Genomics Project, National Cancer Center Research Institute, Tokyo 104-0045, JapanProteome Bioinformatics Project, National Cancer Center Research Institute, Tokyo 104-0045, JapanDepartment of Molecular Cytogenetics, Medical Research Institute and School of Biomedical Science, Tokyo Medical and Dental University, Tokyo 113-8510, JapanDepartment of Molecular Cytogenetics, Medical Research Institute and School of Biomedical Science, Tokyo Medical and Dental University, Tokyo 113-8510, JapanDepartment of Molecular Cytogenetics, Medical Research Institute and School of Biomedical Science, Tokyo Medical and Dental University, Tokyo 113-8510, JapanPathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, JapanTo establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tissue (T) from noncancerous pancreatic tissue in the learning cohort with a specificity of 100%, were identified. Using criteria that combined the 12 BAC clones, T-samples were diagnosed as cancers with 100% sensitivity and specificity in both the learning and validation cohorts. DNA methylation status on 11 of the BAC clones, which was able to discriminate patients showing early relapse from those with no relapse in the learning cohort with 100% specificity, was correlated with the recurrence-free and overall survival rates in the validation cohort and was an independent prognostic factor by multivariate analysis. Genome-wide DNA methylation profiling may provide optimal diagnostic markers and prognostic indicators for patients with PCs.http://dx.doi.org/10.1155/2011/780836
collection DOAJ
language English
format Article
sources DOAJ
author Masahiro Gotoh
Eri Arai
Saori Wakai-Ushijima
Nobuyoshi Hiraoka
Tomoo Kosuge
Fumie Hosoda
Tatsuhiro Shibata
Tadashi Kondo
Sana Yokoi
Issei Imoto
Johji Inazawa
Yae Kanai
spellingShingle Masahiro Gotoh
Eri Arai
Saori Wakai-Ushijima
Nobuyoshi Hiraoka
Tomoo Kosuge
Fumie Hosoda
Tatsuhiro Shibata
Tadashi Kondo
Sana Yokoi
Issei Imoto
Johji Inazawa
Yae Kanai
Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification
Journal of Biomedicine and Biotechnology
author_facet Masahiro Gotoh
Eri Arai
Saori Wakai-Ushijima
Nobuyoshi Hiraoka
Tomoo Kosuge
Fumie Hosoda
Tatsuhiro Shibata
Tadashi Kondo
Sana Yokoi
Issei Imoto
Johji Inazawa
Yae Kanai
author_sort Masahiro Gotoh
title Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification
title_short Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification
title_full Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification
title_fullStr Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification
title_full_unstemmed Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification
title_sort diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide dna methylation profiling by bacterial artificial chromosome array-based methylated cpg island amplification
publisher Hindawi Limited
series Journal of Biomedicine and Biotechnology
issn 1110-7243
1110-7251
publishDate 2011-01-01
description To establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tissue (T) from noncancerous pancreatic tissue in the learning cohort with a specificity of 100%, were identified. Using criteria that combined the 12 BAC clones, T-samples were diagnosed as cancers with 100% sensitivity and specificity in both the learning and validation cohorts. DNA methylation status on 11 of the BAC clones, which was able to discriminate patients showing early relapse from those with no relapse in the learning cohort with 100% specificity, was correlated with the recurrence-free and overall survival rates in the validation cohort and was an independent prognostic factor by multivariate analysis. Genome-wide DNA methylation profiling may provide optimal diagnostic markers and prognostic indicators for patients with PCs.
url http://dx.doi.org/10.1155/2011/780836
work_keys_str_mv AT masahirogotoh diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT eriarai diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT saoriwakaiushijima diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT nobuyoshihiraoka diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT tomookosuge diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT fumiehosoda diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT tatsuhiroshibata diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT tadashikondo diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT sanayokoi diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT isseiimoto diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT johjiinazawa diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
AT yaekanai diagnosisandprognosticationofductaladenocarcinomasofthepancreasbasedongenomewidednamethylationprofilingbybacterialartificialchromosomearraybasedmethylatedcpgislandamplification
_version_ 1725078333979361280

Similar Items