Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats
Objective To study the change of STAT1 expression in the anterior cingulate cortex on rats under orth⁃ odontic force, and to further explore the roles of STAT1 and related JAK⁃STAT1 signaling pathway in the mediation and regulation of pain during tooth movement. Methods 112 male Sprague⁃Dawley (SD...
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Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2017-08-01
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doaj-18c3f2a1cf0c4b51bc392cb9d316f9ae2020-11-24T21:43:40ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562096-14562017-08-0125848248710.12016/j.issn.2096⁃1456.2017.08.002Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in ratsZHENG Yi0ZHAO Zhuannong1WANG Yixi2 CAO Yang3LIU Chufeng4Zhuhai Smile Dental ClinicDepartment of Orthodontics, Stomatological Hospital, Southern Medical UniversityDepartment of Orthodontics, Guanghua School of Stomatology, Hospital of Sto⁃ matology, Sun Yat⁃sen UniversityDepartment of Orthodontics, Guanghua School of Stomatology, Hospital of Sto⁃ matology, Sun Yat⁃sen UniversityDepartment of Orthodontics, Stomatological Hospital, Southern Medical UniversityObjective To study the change of STAT1 expression in the anterior cingulate cortex on rats under orth⁃ odontic force, and to further explore the roles of STAT1 and related JAK⁃STAT1 signaling pathway in the mediation and regulation of pain during tooth movement. Methods 112 male Sprague⁃Dawley (SD) rats (225±25 g) were used in this study. They were randomly divided into experimental groups (96 rats) and control groups (16 rats). All rats were in⁃ stalled bilateral maxillary device for tooth movement models. Rats in the experimental groups were applied 80g orth⁃ odontic force on both sides and were divided into six subgroup 4 h, 12 h, 24 h, 2 d, 3 d, 7 d, with 16 rabbits in each subgroup. The control groups were only installed the same orthodontic devices, without the application of orthodontic force. Brain tissue of the anterior cingulate cortex was isolated after 4 h, 12 h, 24 h, 2 d, 3 d, 7 d since experiment, and the expression level of STAT1 and p⁃STAT1 was analyzed with the method of immunofluorescence and PCR. Results For the immunofluorescence result, there was significant difference in STAT1 expression between control groups and dif⁃ ferent experimental groups at different time points in total (P < 0.05). The STAT1 expression amount in the 4 h group decreased significantly when compared with the control group (P < 0.05); to the 2 d group, the difference is still statisti⁃ cally significant (P < 0.01). 3 d group, 7 d group and control group had no statistically significant difference. The STAT1 expression amount in 4 h group, 12 h group, 24 h group was significantly lower than that in 3 d and 7 d groups, differences were statistically significant (P < 0.05). The STAT1 expression in the 2 d group was significantly lower than that of 7 d (42.35 ± 5.77) group, the difference was statistically significant (P < 0.05). There was significant difference in p⁃STAT1 expression between control groups and different experimental groups at different time points in total (F = 623.518, P < 0.05). The p⁃STAT1 expression amount in experimental groups were higher than that in the control group (P < 0.05). The p⁃STAT1 expression in 4 h group was lower than that in 12 h and 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were statistically significant (P < 0.05). The p⁃STAT1 expression in 12 h group was lower than that in 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were statistically significant (P < 0.05). For the PCR result, the expression of mRNA in STAT1 of experimental groups of 4 h, 12 h, 24 h, 2 d, 3 d, 7 d and the control groups were not statistically significant (P > 0.05). Conclusions After apply⁃ ing orthodontic force, the expression of STAT1 decreased transiently and the expression of p⁃STAT1 increased transient⁃ ly. The reduction of STAT1 was probably caused by the phosphorylation of STAT1 and decrease in the translation level of STAT1, rather than changes in the transcriptional levels. The orthodontic pain might be related with the activation of STAT1 into phosphorylated STAT1.http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.08.002Orthodontic forcePainACCSTAT1pSTAT1 |
collection |
DOAJ |
language |
zho |
format |
Article |
sources |
DOAJ |
author |
ZHENG Yi ZHAO Zhuannong WANG Yixi CAO Yang LIU Chufeng |
spellingShingle |
ZHENG Yi ZHAO Zhuannong WANG Yixi CAO Yang LIU Chufeng Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats 口腔疾病防治 Orthodontic force Pain ACC STAT1 pSTAT1 |
author_facet |
ZHENG Yi ZHAO Zhuannong WANG Yixi CAO Yang LIU Chufeng |
author_sort |
ZHENG Yi |
title |
Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats |
title_short |
Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats |
title_full |
Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats |
title_fullStr |
Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats |
title_full_unstemmed |
Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats |
title_sort |
changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats |
publisher |
Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
series |
口腔疾病防治 |
issn |
2096-1456 2096-1456 |
publishDate |
2017-08-01 |
description |
Objective To study the change of STAT1 expression in the anterior cingulate cortex on rats under orth⁃
odontic force, and to further explore the roles of STAT1 and related JAK⁃STAT1 signaling pathway in the mediation and
regulation of pain during tooth movement. Methods 112 male Sprague⁃Dawley (SD) rats (225±25 g) were used in this
study. They were randomly divided into experimental groups (96 rats) and control groups (16 rats). All rats were in⁃
stalled bilateral maxillary device for tooth movement models. Rats in the experimental groups were applied 80g orth⁃
odontic force on both sides and were divided into six subgroup 4 h, 12 h, 24 h, 2 d, 3 d, 7 d, with 16 rabbits in each
subgroup. The control groups were only installed the same orthodontic devices, without the application of orthodontic
force. Brain tissue of the anterior cingulate cortex was isolated after 4 h, 12 h, 24 h, 2 d, 3 d, 7 d since experiment, and
the expression level of STAT1 and p⁃STAT1 was analyzed with the method of immunofluorescence and PCR. Results
For the immunofluorescence result, there was significant difference in STAT1 expression between control groups and dif⁃
ferent experimental groups at different time points in total (P < 0.05). The STAT1 expression amount in the 4 h group
decreased significantly when compared with the control group (P < 0.05); to the 2 d group, the difference is still statisti⁃
cally significant (P < 0.01). 3 d group, 7 d group and control group had no statistically significant difference. The
STAT1 expression amount in 4 h group, 12 h group, 24 h group was significantly lower than that in 3 d and 7 d groups,
differences were statistically significant (P < 0.05). The STAT1 expression in the 2 d group was significantly lower than
that of 7 d (42.35 ± 5.77) group, the difference was statistically significant (P < 0.05). There was significant difference
in p⁃STAT1 expression between control groups and different experimental groups at different time points in total (F =
623.518, P < 0.05). The p⁃STAT1 expression amount in experimental groups were higher than that in the control group
(P < 0.05). The p⁃STAT1 expression in 4 h group was lower than that in 12 h and 24 h group and higher than that in 2 d,
3 d and 7 d groups, of which the differences were statistically significant (P < 0.05). The p⁃STAT1 expression in 12 h
group was lower than that in 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were
statistically significant (P < 0.05). For the PCR result, the expression of mRNA in STAT1 of experimental groups of 4 h,
12 h, 24 h, 2 d, 3 d, 7 d and the control groups were not statistically significant (P > 0.05). Conclusions After apply⁃
ing orthodontic force, the expression of STAT1 decreased transiently and the expression of p⁃STAT1 increased transient⁃
ly. The reduction of STAT1 was probably caused by the phosphorylation of STAT1 and decrease in the translation level
of STAT1, rather than changes in the transcriptional levels. The orthodontic pain might be related with the activation of
STAT1 into phosphorylated STAT1. |
topic |
Orthodontic force Pain ACC STAT1 pSTAT1 |
url |
http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.08.002 |
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