Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene

Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene

Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The a...

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Main Authors: Munenari Itoh, Shiho Kawagoe, Katsuto Tamai, Hirotaka James Okano, Hidemi Nakagawa
Format: Article
Language:English
Published: Elsevier 2016-07-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116300344
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spelling doaj-18d20fb586a247119c0406556fe6c76b2020-11-25T01:08:14ZengElsevierStem Cell Research1873-50611876-77532016-07-01171323510.1016/j.scr.2016.05.003Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 geneMunenari Itoh0Shiho Kawagoe1Katsuto Tamai2Hirotaka James Okano3Hidemi Nakagawa4Department of Dermatology, The Jikei University School of Medicine, JapanDepartment of Dermatology, The Jikei University School of Medicine, JapanDepartment of Stem Cell Therapy Science, Graduate School of Medicine, Osaka University, JapanDivision of Regenerative Medicine, The Jikei University School of Medicine, JapanDepartment of Dermatology, The Jikei University School of Medicine, JapanExpanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of established iPSC line, RDEB-iPSC26, was confirmed by the expressions of stem cell markers and the differentiation capability into three germ layer. RDEB-iPSC26 may be a useful cell resource for the establishment of in vitro RDEB modeling and the study for developing gene and cell therapy.http://www.sciencedirect.com/science/article/pii/S1873506116300344
collection DOAJ
language English
format Article
sources DOAJ
author Munenari Itoh
Shiho Kawagoe
Katsuto Tamai
Hirotaka James Okano
Hidemi Nakagawa
spellingShingle Munenari Itoh
Shiho Kawagoe
Katsuto Tamai
Hirotaka James Okano
Hidemi Nakagawa
Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene
Stem Cell Research
author_facet Munenari Itoh
Shiho Kawagoe
Katsuto Tamai
Hirotaka James Okano
Hidemi Nakagawa
author_sort Munenari Itoh
title Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene
title_short Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene
title_full Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene
title_fullStr Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene
title_full_unstemmed Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene
title_sort integration-free t cell-derived human induced pluripotent stem cells (ipscs) from a patient with recessive dystrophic epidermolysis bullosa (rdeb) carrying two compound heterozygous mutations in the col7a1 gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-07-01
description Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of established iPSC line, RDEB-iPSC26, was confirmed by the expressions of stem cell markers and the differentiation capability into three germ layer. RDEB-iPSC26 may be a useful cell resource for the establishment of in vitro RDEB modeling and the study for developing gene and cell therapy.
url http://www.sciencedirect.com/science/article/pii/S1873506116300344
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AT shihokawagoe integrationfreetcellderivedhumaninducedpluripotentstemcellsipscsfromapatientwithrecessivedystrophicepidermolysisbullosardebcarryingtwocompoundheterozygousmutationsinthecol7a1gene
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