Eukaryotic Translation Initiation Factor 4AI: A Potential Novel Target in Neuroblastoma
Neuroblastoma (NB) is the most common extracranial pediatric solid tumor. Children suffering from high-risk and/or metastatic NB often show no response to therapy, and new therapeutic approaches are urgently needed. Malignant tumor development has been shown to be driven by the dysregulation of euka...
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doaj-18f806aa7e4e4295a8c835c5577e2fb82021-02-03T00:01:54ZengMDPI AGCells2073-44092021-02-011030130110.3390/cells10020301Eukaryotic Translation Initiation Factor 4AI: A Potential Novel Target in NeuroblastomaChristina Skofler0Florian Kleinegger1Stefanie Krassnig2Anna Maria Birkl-Toeglhofer3Georg Singer4Holger Till5Martin Benesch6Regina Cencic7John A. Porco8Jerry Pelletier9Christoph Castellani10Andrea Raicht11Ewa Izycka-Swieszewska12Piotr Czapiewski13Johannes Haybaeck14Diagnostic and Research Center for Molecular BioMedicine, Diagnostic and Research Institute of Pathology, Medical University of Graz, 8010 Graz, AustriaDiagnostic and Research Center for Molecular BioMedicine, Diagnostic and Research Institute of Pathology, Medical University of Graz, 8010 Graz, AustriaDiagnostic and Research Center for Molecular BioMedicine, Diagnostic and Research Institute of Pathology, Medical University of Graz, 8010 Graz, AustriaDiagnostic and Research Center for Molecular BioMedicine, Diagnostic and Research Institute of Pathology, Medical University of Graz, 8010 Graz, AustriaDepartment of Pediatric and Adolescent Surgery, Medical University of Graz, 8036 Graz, AustriaDepartment of Pediatric and Adolescent Surgery, Medical University of Graz, 8036 Graz, AustriaDepartment of Pediatrics and Adolescent Medicine, Division of Pediatric Hematology/Oncology, Medical University of Graz, 8036 Graz, AustriaMcIntyre Medical Sciences Building, Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, CanadaDepartment of Chemistry and Center for Molecular Discovery (BU-CMD), Boston University, Boston, MA 02215, USAMcIntyre Medical Sciences Building, Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, CanadaDepartment of Pediatric and Adolescent Surgery, Medical University of Graz, 8036 Graz, AustriaDepartment of Pediatrics and Adolescent Medicine, Division of Pediatric Hematology/Oncology, Medical University of Graz, 8036 Graz, AustriaDepartment of Pathology and Neuropathology, Medical University of Gdańsk, 80-211 Gdańsk, PolandDepartment of Pathomorphology, Medical University of Gdańsk, 80-214 Gdańsk, PolandDiagnostic and Research Center for Molecular BioMedicine, Diagnostic and Research Institute of Pathology, Medical University of Graz, 8010 Graz, AustriaNeuroblastoma (NB) is the most common extracranial pediatric solid tumor. Children suffering from high-risk and/or metastatic NB often show no response to therapy, and new therapeutic approaches are urgently needed. Malignant tumor development has been shown to be driven by the dysregulation of eukaryotic initiation factors (eIFs) at the translation initiation. Especially the activity of the heterotrimeric eIF4F complex is often altered in malignant cells, since it is the direct connection to key oncogenic signaling pathways such as the PI3K/AKT/mTOR-pathway. A large body of literature exists that demonstrates targeting the translational machinery as a promising anti-neoplastic approach. The objective of this study was to determine whether eIF4F complex members are aberrantly expressed in NB and whether targeting parts of the complex may be a therapeutic strategy against NB. We show that eIF4AI is overexpressed in NB patient tissue using immunohistochemistry, immunoblotting, and RT-qPCR. NB cell lines exhibit decreased viability, increased apoptosis rates. as well as changes in cell cycle distribution when treated with the synthetic rocaglate CR-1-31-B, which clamps eIF4A and eIF4F onto mRNA, resulting in a translational block. Additionally, this study reveals that CR-1-31-B is effective against NB cell lines at low nanomolar doses (≤20 nM), which previous studies have been shown do not affect non-malignant cells. Thus, our study provides information of the expression status on eIF4AI in NB and offers initial promising insight into targeting translation initiation as an anti-tumorigenic approach for NB.https://www.mdpi.com/2073-4409/10/2/301neuroblastomaeukaryotic initiation factor 4AI (eIF4AI)rocaglatesCR-1-31-BSH-SY5YKelly |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christina Skofler Florian Kleinegger Stefanie Krassnig Anna Maria Birkl-Toeglhofer Georg Singer Holger Till Martin Benesch Regina Cencic John A. Porco Jerry Pelletier Christoph Castellani Andrea Raicht Ewa Izycka-Swieszewska Piotr Czapiewski Johannes Haybaeck |
spellingShingle |
Christina Skofler Florian Kleinegger Stefanie Krassnig Anna Maria Birkl-Toeglhofer Georg Singer Holger Till Martin Benesch Regina Cencic John A. Porco Jerry Pelletier Christoph Castellani Andrea Raicht Ewa Izycka-Swieszewska Piotr Czapiewski Johannes Haybaeck Eukaryotic Translation Initiation Factor 4AI: A Potential Novel Target in Neuroblastoma Cells neuroblastoma eukaryotic initiation factor 4AI (eIF4AI) rocaglates CR-1-31-B SH-SY5Y Kelly |
author_facet |
Christina Skofler Florian Kleinegger Stefanie Krassnig Anna Maria Birkl-Toeglhofer Georg Singer Holger Till Martin Benesch Regina Cencic John A. Porco Jerry Pelletier Christoph Castellani Andrea Raicht Ewa Izycka-Swieszewska Piotr Czapiewski Johannes Haybaeck |
author_sort |
Christina Skofler |
title |
Eukaryotic Translation Initiation Factor 4AI: A Potential Novel Target in Neuroblastoma |
title_short |
Eukaryotic Translation Initiation Factor 4AI: A Potential Novel Target in Neuroblastoma |
title_full |
Eukaryotic Translation Initiation Factor 4AI: A Potential Novel Target in Neuroblastoma |
title_fullStr |
Eukaryotic Translation Initiation Factor 4AI: A Potential Novel Target in Neuroblastoma |
title_full_unstemmed |
Eukaryotic Translation Initiation Factor 4AI: A Potential Novel Target in Neuroblastoma |
title_sort |
eukaryotic translation initiation factor 4ai: a potential novel target in neuroblastoma |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-02-01 |
description |
Neuroblastoma (NB) is the most common extracranial pediatric solid tumor. Children suffering from high-risk and/or metastatic NB often show no response to therapy, and new therapeutic approaches are urgently needed. Malignant tumor development has been shown to be driven by the dysregulation of eukaryotic initiation factors (eIFs) at the translation initiation. Especially the activity of the heterotrimeric eIF4F complex is often altered in malignant cells, since it is the direct connection to key oncogenic signaling pathways such as the PI3K/AKT/mTOR-pathway. A large body of literature exists that demonstrates targeting the translational machinery as a promising anti-neoplastic approach. The objective of this study was to determine whether eIF4F complex members are aberrantly expressed in NB and whether targeting parts of the complex may be a therapeutic strategy against NB. We show that eIF4AI is overexpressed in NB patient tissue using immunohistochemistry, immunoblotting, and RT-qPCR. NB cell lines exhibit decreased viability, increased apoptosis rates. as well as changes in cell cycle distribution when treated with the synthetic rocaglate CR-1-31-B, which clamps eIF4A and eIF4F onto mRNA, resulting in a translational block. Additionally, this study reveals that CR-1-31-B is effective against NB cell lines at low nanomolar doses (≤20 nM), which previous studies have been shown do not affect non-malignant cells. Thus, our study provides information of the expression status on eIF4AI in NB and offers initial promising insight into targeting translation initiation as an anti-tumorigenic approach for NB. |
topic |
neuroblastoma eukaryotic initiation factor 4AI (eIF4AI) rocaglates CR-1-31-B SH-SY5Y Kelly |
url |
https://www.mdpi.com/2073-4409/10/2/301 |
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