Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia
The Loopamp™ MTBC kit (TB-LAMP) is recommended by WHO for Mycobacterium tuberculosis complex detection in low-income countries with a still low drug-resistant tuberculosis (TB) rate. This study is aimed at testing its feasibility in Cambodia on sputa collected from presumptive tuberculosis patients....
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2020-01-01
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Online Access: | http://dx.doi.org/10.1155/2020/6828043 |
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doaj-1904b7308649468ab0d5f74181ed656c2020-11-25T03:13:29ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/68280436828043Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in CambodiaSokleaph Cheng0Sok Heng Pheng1Seiha Heng2Guy B. Marks3Anne-Laure Bañuls4Tan Eang Mao5Alexandra Kerléguer6Ministry of Health, Phnom Penh, CambodiaMinistry of Health, Phnom Penh, CambodiaInstitut Pasteur du Cambodge, Phnom Penh, CambodiaWoolcock Institute of Medical Research, University of New South Wales, Sydney, AustraliaLMI “Drug Resistance in South East Asia (DRISA)”, Institut Pasteur du Cambodge and Institut de Recherche pour le Développement, Phnom Penh, CambodiaMinistry of Health, Phnom Penh, CambodiaInstitut Pasteur du Cambodge, Phnom Penh, CambodiaThe Loopamp™ MTBC kit (TB-LAMP) is recommended by WHO for Mycobacterium tuberculosis complex detection in low-income countries with a still low drug-resistant tuberculosis (TB) rate. This study is aimed at testing its feasibility in Cambodia on sputa collected from presumptive tuberculosis patients. 499 samples were tested at a smear microscopy center and 200 at a central-level mycobacteriology laboratory. Using mycobacterial cultures as reference, TB-LAMP results were compared with those of LED fluorescent microscopy (LED-FM) and Xpert® MTB/RIF. At the microscopy center, TB-LAMP sensitivity was higher than that of LED-FM (81.5% [95% CI, 74.5-87.6] versus 69.4% [95% CI, 62.2-76.6]), but lower than that of the Xpert assay (95.5% [95% CI 92.3-98.8]). At the central-level laboratory, TB-LAMP sensitivity (92.8% [95% CI, 87.6-97.9]) was comparable to that of Xpert (90.7% [95% CI, 85.0-96.5]) using stored sample. No significant difference in terms of specificity between TB-LAMP and Xpert assays was observed in both study sites. In conclusion, our data demonstrate that TB-LAMP could be implemented at microscopy centers in Cambodia to detect TB patients. In addition, TB-LAMP can be a better choice to replace smear microscopy for rapid TB diagnosis of new presumptive TB patients, in settings with relative low prevalence of drug-resistant TB and difficulties to implement Xpert assay.http://dx.doi.org/10.1155/2020/6828043 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sokleaph Cheng Sok Heng Pheng Seiha Heng Guy B. Marks Anne-Laure Bañuls Tan Eang Mao Alexandra Kerléguer |
spellingShingle |
Sokleaph Cheng Sok Heng Pheng Seiha Heng Guy B. Marks Anne-Laure Bañuls Tan Eang Mao Alexandra Kerléguer Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia BioMed Research International |
author_facet |
Sokleaph Cheng Sok Heng Pheng Seiha Heng Guy B. Marks Anne-Laure Bañuls Tan Eang Mao Alexandra Kerléguer |
author_sort |
Sokleaph Cheng |
title |
Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia |
title_short |
Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia |
title_full |
Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia |
title_fullStr |
Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia |
title_full_unstemmed |
Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia |
title_sort |
evaluation of loopamp assay for the diagnosis of pulmonary tuberculosis in cambodia |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
The Loopamp™ MTBC kit (TB-LAMP) is recommended by WHO for Mycobacterium tuberculosis complex detection in low-income countries with a still low drug-resistant tuberculosis (TB) rate. This study is aimed at testing its feasibility in Cambodia on sputa collected from presumptive tuberculosis patients. 499 samples were tested at a smear microscopy center and 200 at a central-level mycobacteriology laboratory. Using mycobacterial cultures as reference, TB-LAMP results were compared with those of LED fluorescent microscopy (LED-FM) and Xpert® MTB/RIF. At the microscopy center, TB-LAMP sensitivity was higher than that of LED-FM (81.5% [95% CI, 74.5-87.6] versus 69.4% [95% CI, 62.2-76.6]), but lower than that of the Xpert assay (95.5% [95% CI 92.3-98.8]). At the central-level laboratory, TB-LAMP sensitivity (92.8% [95% CI, 87.6-97.9]) was comparable to that of Xpert (90.7% [95% CI, 85.0-96.5]) using stored sample. No significant difference in terms of specificity between TB-LAMP and Xpert assays was observed in both study sites. In conclusion, our data demonstrate that TB-LAMP could be implemented at microscopy centers in Cambodia to detect TB patients. In addition, TB-LAMP can be a better choice to replace smear microscopy for rapid TB diagnosis of new presumptive TB patients, in settings with relative low prevalence of drug-resistant TB and difficulties to implement Xpert assay. |
url |
http://dx.doi.org/10.1155/2020/6828043 |
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