The effect of ginsenoside Rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomics
Heart failure (HF), with a high fatality rate, is seriously harmful to human health. Ginsenoside Rg5 (Rg5) is the major active component in black ginseng. The effect and mechanism of Rg5 on HF were investigated for the first time. Firstly, the in vitro angiotensin-converting enzyme (ACE) inhibitory...
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doaj-195d13ee852d4f5496486b0a8cab7c402021-04-30T07:20:20ZengElsevierJournal of Functional Foods1756-46462021-01-0176104325The effect of ginsenoside Rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomicsJunli Liu0Yunhe Liu1Hongqiang Lin2Baisong Zhou3Hui Yu4Le Li5Caixia Wang6Xinyu Li7Pingya Li8Jinping Liu9Xiuhua Hao10School of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, China; Research Center of Natural Drug, Jilin University, Changchun 130021, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun 130021, China; Research Center of Natural Drug, Jilin University, Changchun 130021, China; Corresponding authors at: School of Pharmaceutical Sciences, Jilin University, Fujin Road 1266, Changchun 130021, China. (J. Liu)School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China; Corresponding authors at: School of Pharmaceutical Sciences, Jilin University, Fujin Road 1266, Changchun 130021, China. (J. Liu)Heart failure (HF), with a high fatality rate, is seriously harmful to human health. Ginsenoside Rg5 (Rg5) is the major active component in black ginseng. The effect and mechanism of Rg5 on HF were investigated for the first time. Firstly, the in vitro angiotensin-converting enzyme (ACE) inhibitory activity was evaluated. Then, the verapamil-induced zebrafish HF model was used to assay the effect of Rg5. Finally, the untargeted metabolomics based on UPLC-QTOF-MS was applied to explore the latent mechanism by analyzing the metabolic perturbation. The results showed that Rg5 had a similar ACE-inhibitory activity (IC50 = 0.124 μM) to the reference drug enalapril. Rg5 could dramatically improve cardiac function in a dose-dependent manner. A total of 22 differentiated metabolites and 8 perturbed metabolic pathways were identified. In summary, this study indicated that Rg5 could be a potential agent for protecting heart function in the clinical treatment of heart failure.http://www.sciencedirect.com/science/article/pii/S1756464620305491Ginsenoside Rg5ACE inhibitorZebrafishHeart failureMetabolomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Junli Liu Yunhe Liu Hongqiang Lin Baisong Zhou Hui Yu Le Li Caixia Wang Xinyu Li Pingya Li Jinping Liu Xiuhua Hao |
spellingShingle |
Junli Liu Yunhe Liu Hongqiang Lin Baisong Zhou Hui Yu Le Li Caixia Wang Xinyu Li Pingya Li Jinping Liu Xiuhua Hao The effect of ginsenoside Rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomics Journal of Functional Foods Ginsenoside Rg5 ACE inhibitor Zebrafish Heart failure Metabolomics |
author_facet |
Junli Liu Yunhe Liu Hongqiang Lin Baisong Zhou Hui Yu Le Li Caixia Wang Xinyu Li Pingya Li Jinping Liu Xiuhua Hao |
author_sort |
Junli Liu |
title |
The effect of ginsenoside Rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomics |
title_short |
The effect of ginsenoside Rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomics |
title_full |
The effect of ginsenoside Rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomics |
title_fullStr |
The effect of ginsenoside Rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomics |
title_full_unstemmed |
The effect of ginsenoside Rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomics |
title_sort |
effect of ginsenoside rg5, isolated from black ginseng, on heart failure in zebrafish based on untargeted metabolomics |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2021-01-01 |
description |
Heart failure (HF), with a high fatality rate, is seriously harmful to human health. Ginsenoside Rg5 (Rg5) is the major active component in black ginseng. The effect and mechanism of Rg5 on HF were investigated for the first time. Firstly, the in vitro angiotensin-converting enzyme (ACE) inhibitory activity was evaluated. Then, the verapamil-induced zebrafish HF model was used to assay the effect of Rg5. Finally, the untargeted metabolomics based on UPLC-QTOF-MS was applied to explore the latent mechanism by analyzing the metabolic perturbation. The results showed that Rg5 had a similar ACE-inhibitory activity (IC50 = 0.124 μM) to the reference drug enalapril. Rg5 could dramatically improve cardiac function in a dose-dependent manner. A total of 22 differentiated metabolites and 8 perturbed metabolic pathways were identified. In summary, this study indicated that Rg5 could be a potential agent for protecting heart function in the clinical treatment of heart failure. |
topic |
Ginsenoside Rg5 ACE inhibitor Zebrafish Heart failure Metabolomics |
url |
http://www.sciencedirect.com/science/article/pii/S1756464620305491 |
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