Nucleotide Sequence and Phylogenetic Analysis of Glycoprotein-G of the Russian Fixed Rabies Virus Strain “Moscow 3253”

• Main Authors: I. V. Tuchkov, Ya. M. Krasnov, A. A. Goryaev, Zh. V. Matveeva, A. V. Stepanov, N. V. Mayorov, A. K. Nikiforov
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2013-12-01
• Series: Проблемы особо опасных инфекций
• Subjects: вирус бешенства; филогенетический анализ; нуклеотидная последовательность; полимеразная цепная реакция; сиквенирование днк; rabies virus; phylogenetic analysis; nucleotide sequence; polymerase chain reaction; dna sequencing
• Online Access: https://journal.microbe.ru/jour/article/view/82

Fully sequenced have been glycoprotein-G, sha-psi region, as well as H-end site of the L-gene in the rabies virus strain “Moscow 3253”. Compared are amino acid sequences of proteins of “Moscow 3253” strain and other fixed strains of the virus. Established is 98 % DNA homology with RV-97, and 91% homology with PV (Pasteur virus) strain. Constructed has been phylogenetic tree of the strain under study alongside with various groups of fixed rabies virus. It is revealed that “Moscow 3253” strain has closer genetic relations with Japanese group of strains, than with PV strain. Put forward is an assumption that PV strain does not derive from the virus isolated by Pasteur, but relates to the American group of strains.