Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus
Despite increasing research in type 2 diabetes mellitus (T2D), there are few studies showing the impact of the poor glycemic control on biological processes occurring in T2D. In order to identify potential genes related to poorly/well-controlled patients with T2D, our strategy of investigation inclu...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2017-01-01
|
Series: | Journal of Diabetes Research |
Online Access: | http://dx.doi.org/10.1155/2017/2180819 |
id |
doaj-196c20bd76ba40bf8a38aa0ba8fd4128 |
---|---|
record_format |
Article |
spelling |
doaj-196c20bd76ba40bf8a38aa0ba8fd41282020-11-24T23:11:33ZengHindawi LimitedJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/21808192180819Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes MellitusSâmia Cruz Tfaile Corbi0Alliny Souza Bastos1Rafael Nepomuceno2Thamiris Cirelli3Raquel Alves dos Santos4Catarina Satie Takahashi5Cristiane S. Rocha6Silvana Regina Perez Orrico7Claudia V. Maurer-Morelli8Raquel Mantuaneli Scarel-Caminaga9Department of Diagnosis and Surgery, School of Dentistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilPostgraduate Program in Sciences of the University of Franca, Franca, SP, BrazilDepartment of Genetics, Faculty of Medicine of Ribeirão Preto and Department of Biology, FFCLRP, University of São Paulo (USP), Ribeirão Preto, SP, BrazilDepartment of Medical Genetics, University of Campinas (UNICAMP), Campinas, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Medical Genetics, University of Campinas (UNICAMP), Campinas, SP, BrazilDepartment of Morphology, School of Dentistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilDespite increasing research in type 2 diabetes mellitus (T2D), there are few studies showing the impact of the poor glycemic control on biological processes occurring in T2D. In order to identify potential genes related to poorly/well-controlled patients with T2D, our strategy of investigation included a primary screen by microarray (Human Genome U133) in a small group of individuals followed by an independent validation in a greater group using RT-qPCR. Ninety patients were divided as follows: poorly controlled T2D (G1), well-controlled T2D (G2), and normoglycemic individuals (G3). After using affy package in R, differentially expressed genes (DEGs) were prospected as candidate genes potentially relevant for the glycemic control in T2D patients. After validation by RT-qPCR, the obtained DEGs were as follows—G1 + G2 versus G3: HLA-DQA1, SOS1, and BRCA2; G2 versus G1: ENO2, VAMP2, CCND3, CEBPD, LGALS12, AGBL5, MAP2K5, and PPAP2B; G2 versus G3: HLA-DQB1, MCM4, and SEC13; and G1 versus G3: PPIC. This demonstrated a systemic exacerbation of the gene expression related to immune response in T2D patients. Moreover, genes related to lipid metabolisms and DNA replication/repair were influenced by the glycemic control. In conclusion, this study pointed out candidate genes potentially associated with adequate glycemic control in T2D patients, contributing to the knowledge of how the glycemic control could systemically influence gene expression.http://dx.doi.org/10.1155/2017/2180819 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sâmia Cruz Tfaile Corbi Alliny Souza Bastos Rafael Nepomuceno Thamiris Cirelli Raquel Alves dos Santos Catarina Satie Takahashi Cristiane S. Rocha Silvana Regina Perez Orrico Claudia V. Maurer-Morelli Raquel Mantuaneli Scarel-Caminaga |
spellingShingle |
Sâmia Cruz Tfaile Corbi Alliny Souza Bastos Rafael Nepomuceno Thamiris Cirelli Raquel Alves dos Santos Catarina Satie Takahashi Cristiane S. Rocha Silvana Regina Perez Orrico Claudia V. Maurer-Morelli Raquel Mantuaneli Scarel-Caminaga Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus Journal of Diabetes Research |
author_facet |
Sâmia Cruz Tfaile Corbi Alliny Souza Bastos Rafael Nepomuceno Thamiris Cirelli Raquel Alves dos Santos Catarina Satie Takahashi Cristiane S. Rocha Silvana Regina Perez Orrico Claudia V. Maurer-Morelli Raquel Mantuaneli Scarel-Caminaga |
author_sort |
Sâmia Cruz Tfaile Corbi |
title |
Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus |
title_short |
Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus |
title_full |
Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus |
title_fullStr |
Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus |
title_full_unstemmed |
Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus |
title_sort |
expression profile of genes potentially associated with adequate glycemic control in patients with type 2 diabetes mellitus |
publisher |
Hindawi Limited |
series |
Journal of Diabetes Research |
issn |
2314-6745 2314-6753 |
publishDate |
2017-01-01 |
description |
Despite increasing research in type 2 diabetes mellitus (T2D), there are few studies showing the impact of the poor glycemic control on biological processes occurring in T2D. In order to identify potential genes related to poorly/well-controlled patients with T2D, our strategy of investigation included a primary screen by microarray (Human Genome U133) in a small group of individuals followed by an independent validation in a greater group using RT-qPCR. Ninety patients were divided as follows: poorly controlled T2D (G1), well-controlled T2D (G2), and normoglycemic individuals (G3). After using affy package in R, differentially expressed genes (DEGs) were prospected as candidate genes potentially relevant for the glycemic control in T2D patients. After validation by RT-qPCR, the obtained DEGs were as follows—G1 + G2 versus G3: HLA-DQA1, SOS1, and BRCA2; G2 versus G1: ENO2, VAMP2, CCND3, CEBPD, LGALS12, AGBL5, MAP2K5, and PPAP2B; G2 versus G3: HLA-DQB1, MCM4, and SEC13; and G1 versus G3: PPIC. This demonstrated a systemic exacerbation of the gene expression related to immune response in T2D patients. Moreover, genes related to lipid metabolisms and DNA replication/repair were influenced by the glycemic control. In conclusion, this study pointed out candidate genes potentially associated with adequate glycemic control in T2D patients, contributing to the knowledge of how the glycemic control could systemically influence gene expression. |
url |
http://dx.doi.org/10.1155/2017/2180819 |
work_keys_str_mv |
AT samiacruztfailecorbi expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT allinysouzabastos expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT rafaelnepomuceno expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT thamiriscirelli expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT raquelalvesdossantos expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT catarinasatietakahashi expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT cristianesrocha expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT silvanareginaperezorrico expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT claudiavmaurermorelli expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus AT raquelmantuaneliscarelcaminaga expressionprofileofgenespotentiallyassociatedwithadequateglycemiccontrolinpatientswithtype2diabetesmellitus |
_version_ |
1725603886930067456 |