Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019
Abstract Background Newcastle disease (ND) causes severe economic losses in poultry industry worldwide. Egyptian poultry industry suffered from severe economic losses since the isolation of Velogenic Newcastle disease virus (vNDV) genotype VIId in 2011 and up till now despite the use of different va...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2020-07-01
|
Series: | BMC Veterinary Research |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12917-020-02470-9 |
id |
doaj-1983b24b4b26496db9300238ab2e62fb |
---|---|
record_format |
Article |
spelling |
doaj-1983b24b4b26496db9300238ab2e62fb2020-11-25T03:39:10ZengBMCBMC Veterinary Research1746-61482020-07-0116111510.1186/s12917-020-02470-9Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019Hatem S. Abd El-Hamid0Manal E. Shafi1Najah M. Albaqami2Hany F. Ellakany3Naglaa M. Abdelaziz4Mohamed N. Abdelaziz5Mohamed E. Abd El-Hack6Ayman E. Taha7Khalid M. Alanazi8Ahmed R. Elbestawy9Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour UniversityDepartment of Biological Sciences, Zoology, King Abdulaziz UniversityDepartment of Biological Sciences, Zoology, King Abdulaziz UniversityDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour UniversityReference Laboratory for veterinary Quality control on Poultry production (RLQP), Animal Health Research InstituteReference Laboratory for veterinary Quality control on Poultry production (RLQP), Animal Health Research InstituteDepartment of Poultry, Faculty of Agriculture, Zagazig UniversityDepartment of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria UniversityZoology Department, College of Science, King Saud UniversityDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour UniversityAbstract Background Newcastle disease (ND) causes severe economic losses in poultry industry worldwide. Egyptian poultry industry suffered from severe economic losses since the isolation of Velogenic Newcastle disease virus (vNDV) genotype VIId in 2011 and up till now despite the use of different vaccination programs. So, this study aimed to isolate and characterize the vNDV from a total of 120 poultry flocks from ten provinces in the Egyptian Delta region with a history of respiratory manifestation, high mortalities or a decrease in egg production between 2015 and 2019. Seventy-three samples’ allantoic fluid (73/120, 60.8%) were positive for hemagglutination with chicken RBCs. These samples were submitted to molecular examination using qRT-PCR specific primers for AOAV-1, highly pathogenic avian influenza (HPAI-H5), low pathogenic avian influenza (LPAI-H9) and infectious bronchitis virus (IBV). Results Fifty samples (50/120: 41.6%) were confirmed positive for AOAV-1, based on genetic analysis of matrix and fusion protein. The co-infection rate of other respiratory viral diseases examined was 1.6, 14.1, and 4.1%, for HPAI-H5, LPAI-H9, and IBV, respectively. Biologically, the intracerebral pathogenicity index of ten selected AOAV-1 isolates ranged from 1.70 to 1.98, which indicated the velogenic nature of these isolates. All the sixteen sequenced isolates were AOAV-1 genotype VII.1.1. The full F gene sequence of six examined AOAV-1 VII.1.1 isolates contained the seven neutralizing epitopes, and the glycosylation motif of six-potential sites for N linked glycosylation at residues 85, 191, 366, 447, 471, and 541. Conclusion It could be concluded that the high prevalence of AOAV-1 genotype VII.1.1 in the Egyptian chicken flocks despite the intensive vaccination with live and killed ND vaccines, as all the 16 isolates tested were belonged to this genotype. Homologous vaccination is badly needed to control and reduce the spread of AOAV-1 genotype VII.1.1infection in Egyptian poultry flocks.http://link.springer.com/article/10.1186/s12917-020-02470-9Avian Orthoavulavirus 1F proteinSequencingAmino acid residue substitutionIntracerebral |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hatem S. Abd El-Hamid Manal E. Shafi Najah M. Albaqami Hany F. Ellakany Naglaa M. Abdelaziz Mohamed N. Abdelaziz Mohamed E. Abd El-Hack Ayman E. Taha Khalid M. Alanazi Ahmed R. Elbestawy |
spellingShingle |
Hatem S. Abd El-Hamid Manal E. Shafi Najah M. Albaqami Hany F. Ellakany Naglaa M. Abdelaziz Mohamed N. Abdelaziz Mohamed E. Abd El-Hack Ayman E. Taha Khalid M. Alanazi Ahmed R. Elbestawy Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019 BMC Veterinary Research Avian Orthoavulavirus 1 F protein Sequencing Amino acid residue substitution Intracerebral |
author_facet |
Hatem S. Abd El-Hamid Manal E. Shafi Najah M. Albaqami Hany F. Ellakany Naglaa M. Abdelaziz Mohamed N. Abdelaziz Mohamed E. Abd El-Hack Ayman E. Taha Khalid M. Alanazi Ahmed R. Elbestawy |
author_sort |
Hatem S. Abd El-Hamid |
title |
Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019 |
title_short |
Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019 |
title_full |
Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019 |
title_fullStr |
Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019 |
title_full_unstemmed |
Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019 |
title_sort |
sequence analysis and pathogenicity of avian orthoavulavirus 1 strains isolated from poultry flocks during 2015–2019 |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2020-07-01 |
description |
Abstract Background Newcastle disease (ND) causes severe economic losses in poultry industry worldwide. Egyptian poultry industry suffered from severe economic losses since the isolation of Velogenic Newcastle disease virus (vNDV) genotype VIId in 2011 and up till now despite the use of different vaccination programs. So, this study aimed to isolate and characterize the vNDV from a total of 120 poultry flocks from ten provinces in the Egyptian Delta region with a history of respiratory manifestation, high mortalities or a decrease in egg production between 2015 and 2019. Seventy-three samples’ allantoic fluid (73/120, 60.8%) were positive for hemagglutination with chicken RBCs. These samples were submitted to molecular examination using qRT-PCR specific primers for AOAV-1, highly pathogenic avian influenza (HPAI-H5), low pathogenic avian influenza (LPAI-H9) and infectious bronchitis virus (IBV). Results Fifty samples (50/120: 41.6%) were confirmed positive for AOAV-1, based on genetic analysis of matrix and fusion protein. The co-infection rate of other respiratory viral diseases examined was 1.6, 14.1, and 4.1%, for HPAI-H5, LPAI-H9, and IBV, respectively. Biologically, the intracerebral pathogenicity index of ten selected AOAV-1 isolates ranged from 1.70 to 1.98, which indicated the velogenic nature of these isolates. All the sixteen sequenced isolates were AOAV-1 genotype VII.1.1. The full F gene sequence of six examined AOAV-1 VII.1.1 isolates contained the seven neutralizing epitopes, and the glycosylation motif of six-potential sites for N linked glycosylation at residues 85, 191, 366, 447, 471, and 541. Conclusion It could be concluded that the high prevalence of AOAV-1 genotype VII.1.1 in the Egyptian chicken flocks despite the intensive vaccination with live and killed ND vaccines, as all the 16 isolates tested were belonged to this genotype. Homologous vaccination is badly needed to control and reduce the spread of AOAV-1 genotype VII.1.1infection in Egyptian poultry flocks. |
topic |
Avian Orthoavulavirus 1 F protein Sequencing Amino acid residue substitution Intracerebral |
url |
http://link.springer.com/article/10.1186/s12917-020-02470-9 |
work_keys_str_mv |
AT hatemsabdelhamid sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT manaleshafi sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT najahmalbaqami sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT hanyfellakany sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT naglaamabdelaziz sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT mohamednabdelaziz sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT mohamedeabdelhack sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT aymanetaha sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT khalidmalanazi sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 AT ahmedrelbestawy sequenceanalysisandpathogenicityofavianorthoavulavirus1strainsisolatedfrompoultryflocksduring20152019 |
_version_ |
1724540483627122688 |