Trans-Stilbenes in Commercial Grape Juices: Quantification Using HPLC Approaches

• Main Authors: Julia López-Hernández, Ana Rodríguez-Bernaldo de Quirós
• Format: Article
• Language: English
• Published: MDPI AG 2016-10-01
• Series: International Journal of Molecular Sciences
• Subjects: trans-stilbenes; commercial fruit juices; high-performance liquid chromatography; variable wavelength detector; fluorescence detector
• Online Access: http://www.mdpi.com/1422-0067/17/10/1769

Trans-stilbenes belong to the group of polyphenolic phytoalexins, and occur in many plant foods. These compounds have received great attention by researchers due to their well-known beneficial health effects. In the present study a chromatographic method that comprises the use of variable wavelength (VWD) and fluorescence (FLD) detectors in series for the analysis of trans-stilbenes is presented. The relation of peak-area obtained with both detectors is proposed as an alternative and complementary approach for the rapid identification of these phenolic compounds. The proposed method was applied to determine trans-stilbenes in commercial fruit juices. Trans-piceid was the most common trans-stilbene found in the samples analyzed. The method was validated in terms of linearity, sensitivity and repeatability. Appropriate sensitivity and good linearity (r2 > 0.9991) were achieved.