Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI
Abstract Background The complex relationship between oocyte morphology, specific follicular fluid metabolites, gene expression in cumulus granulosa cells, and oocyte competence toward fertilization and embryo development still needs further clarification. Methods Forty-six oocytes retrieved from the...
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doaj-198ac1e936954560a9cfcafcc2eb6e7c2020-11-24T20:56:09ZengBMCReproductive Biology and Endocrinology1477-78272017-06-0115111210.1186/s12958-017-0265-2Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSIAlberto Revelli0Stefano Canosa1Loredana Bergandi2Oleksii A. Skorokhod3Valentina Biasoni4Andrea Carosso5Angela Bertagna6Milena Maule7Elisabetta Aldieri8Maria Diletta D’Eufemia9Francesca Evangelista10Nicola Colacurci11Chiara Benedetto12Chair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of TorinoChair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of TorinoDepartment of Oncology, University of TorinoDepartment of Oncology, University of TorinoChair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of TorinoChair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of TorinoEndocrinology Laboratory, Department of Internal Medicine, University of TorinoDepartment of Medical Sciences, Cancer Epidemiology Unit, University of TorinoDepartment of Oncology, University of TorinoDepartment of Woman, Child, General and Special Surgery, Second University of NapoliChair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of TorinoDepartment of Woman, Child, General and Special Surgery, Second University of NapoliChair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of TorinoAbstract Background The complex relationship between oocyte morphology, specific follicular fluid metabolites, gene expression in cumulus granulosa cells, and oocyte competence toward fertilization and embryo development still needs further clarification. Methods Forty-six oocytes retrieved from the largest pre-ovulatory follicle of patients undergoing intra-cytoplasmic sperm injection (ICSI) were considered assessing: (a) oocyte morphological characteristics at polarized light microscopy (PLM), (b) specific follicular fluid (FF) metabolites previously suggested to influence oocyte competence (AMH, markers of redox status and of cytotoxicity), (c) transcription of AMH and AMH type II receptor genes in cumulus cells. Data were analyzed using mono-parametric tests and multivariable logistic analysis in order to correlate morphological and biochemical data with fertilization. Results Comparing normally fertilized oocytes (n = 29, F group) with unfertilized (n = 17, nF group) we observed that: (a) the meiotic spindle area and major axis were significantly higher in nF group and in fertilized oocytes undergoing an early embryo development arrest; (b) AMH level in FF was comparable in F and nF groups; (c) the FF of nF group contained significantly higher levels of cytotoxicity (lactate dehydrogenase) and oxidative stress (Cu,Zn-superoxide dismutase, catalase, 4-hydroxynonenal-protein conjugates) markers; (d) cumulus cells of nF group showed significantly higher AMH receptor type II gene expression. Conclusions Taken together, these observations suggest that an excessive cytotoxicity level can alter AMH signal transduction within cumulus cells, in turn leading to partial inhibition of aromatase activity, altered cytoplasmic maturation and increased oxidative stress, factors able to impair oocyte fertilization competence and embryo growth.http://link.springer.com/article/10.1186/s12958-017-0265-2OocyteFertilizationFollicular fluidCumulus cellsAmhPolarized light microscopy |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alberto Revelli Stefano Canosa Loredana Bergandi Oleksii A. Skorokhod Valentina Biasoni Andrea Carosso Angela Bertagna Milena Maule Elisabetta Aldieri Maria Diletta D’Eufemia Francesca Evangelista Nicola Colacurci Chiara Benedetto |
spellingShingle |
Alberto Revelli Stefano Canosa Loredana Bergandi Oleksii A. Skorokhod Valentina Biasoni Andrea Carosso Angela Bertagna Milena Maule Elisabetta Aldieri Maria Diletta D’Eufemia Francesca Evangelista Nicola Colacurci Chiara Benedetto Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI Reproductive Biology and Endocrinology Oocyte Fertilization Follicular fluid Cumulus cells Amh Polarized light microscopy |
author_facet |
Alberto Revelli Stefano Canosa Loredana Bergandi Oleksii A. Skorokhod Valentina Biasoni Andrea Carosso Angela Bertagna Milena Maule Elisabetta Aldieri Maria Diletta D’Eufemia Francesca Evangelista Nicola Colacurci Chiara Benedetto |
author_sort |
Alberto Revelli |
title |
Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI |
title_short |
Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI |
title_full |
Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI |
title_fullStr |
Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI |
title_full_unstemmed |
Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI |
title_sort |
oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after icsi |
publisher |
BMC |
series |
Reproductive Biology and Endocrinology |
issn |
1477-7827 |
publishDate |
2017-06-01 |
description |
Abstract Background The complex relationship between oocyte morphology, specific follicular fluid metabolites, gene expression in cumulus granulosa cells, and oocyte competence toward fertilization and embryo development still needs further clarification. Methods Forty-six oocytes retrieved from the largest pre-ovulatory follicle of patients undergoing intra-cytoplasmic sperm injection (ICSI) were considered assessing: (a) oocyte morphological characteristics at polarized light microscopy (PLM), (b) specific follicular fluid (FF) metabolites previously suggested to influence oocyte competence (AMH, markers of redox status and of cytotoxicity), (c) transcription of AMH and AMH type II receptor genes in cumulus cells. Data were analyzed using mono-parametric tests and multivariable logistic analysis in order to correlate morphological and biochemical data with fertilization. Results Comparing normally fertilized oocytes (n = 29, F group) with unfertilized (n = 17, nF group) we observed that: (a) the meiotic spindle area and major axis were significantly higher in nF group and in fertilized oocytes undergoing an early embryo development arrest; (b) AMH level in FF was comparable in F and nF groups; (c) the FF of nF group contained significantly higher levels of cytotoxicity (lactate dehydrogenase) and oxidative stress (Cu,Zn-superoxide dismutase, catalase, 4-hydroxynonenal-protein conjugates) markers; (d) cumulus cells of nF group showed significantly higher AMH receptor type II gene expression. Conclusions Taken together, these observations suggest that an excessive cytotoxicity level can alter AMH signal transduction within cumulus cells, in turn leading to partial inhibition of aromatase activity, altered cytoplasmic maturation and increased oxidative stress, factors able to impair oocyte fertilization competence and embryo growth. |
topic |
Oocyte Fertilization Follicular fluid Cumulus cells Amh Polarized light microscopy |
url |
http://link.springer.com/article/10.1186/s12958-017-0265-2 |
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