Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis

• Main Authors: Wahyu Widowati, Dian Laksmitawati, Teresa Wargasetia, Ervi Afifah, Annisa Amalia, Yukko Arinta, Rizal Rizal, Tri Suciati
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2018-09-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: Fibronectin; Glomerulosclerosis; Garcinia mangostana; Mesangial cell; Transforming growth factor-β1
• Online Access: http://ijbms.mums.ac.ir/article_11203_14e704ac2a5f60db9c0879be5810f630.pdf

Objective(s): This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line (Glomerular Mesangial Kidney, Mus Musculus)).  Materials and Methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, while the level of TGF-β1 (Transforming growth factor-β1) and fibronectin in glucose-induced mesangial cells were assayed and determined using ELISA KIT. Results: In viability assay, MPE 5 and 20 µg/ml has the highest activity to increase cells proliferation in glucose-induced mesangial cells at 5, 10, and 15 days of incubation in glucose concentration (5 and 25 mM) (P