Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data

In this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD...

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Main Authors: Regina Helena Geribello Priolli, José Baldin Pinheiro, Maria Imaculada Zucchi, Miklos Maximiliano Bajay, Natal Antonio Vello
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2010-06-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004
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spelling doaj-1a03f6fc37184040879d2a8f68fa20852020-11-25T02:24:33ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-06-0153351953110.1590/S1516-89132010000300004Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree dataRegina Helena Geribello PriolliJosé Baldin PinheiroMaria Imaculada ZucchiMiklos Maximiliano BajayNatal Antonio VelloIn this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD) was determined using the modified Roger's Wright distance, and a final dendrogram was in agreement with the cultivar pedigree. A distance matrix based on the coefficient of parentage scores was also generated for the cultivars, which ranged from 0 to 1, with a mean of 0.18, whereas SSR-based genetic similarity (1- GD) ranged from 0.01 to 0.90, with a mean of 0.25. Mantel's Z test showed that the similarity matrices generated from both the data sets were low, but significantly correlated (r = 0.31, p<0.001). The results showed that SSR data and pedigree analyses could help to quantify more accurately the degree of relationship among the soybean cultivars.<br>Locos microssatélites e dados de genealogia foram utilizados para avaliar a diversidade genética de um grupo de 168 cultivares brasileiras de soja. Os dezoito locos utilizados apresentaram em média 5,06 alelos por loco e coeficiente de diversidade genética médio de 0,58. O dendrograma final resultante da matriz de distância genética de Roger modificado por Wright, apresentou boa concordância com a ancestralidade dos grupos formados. Também foi estimado os coeficientes de parentesco entre as cultivares, sendo observada variação de 0 a 1 com média de 0,18, enquanto que as similaridades para os locos microssatélites (1- GD) variou de 0,01 a 0,90 com média de 0,25. A correlação entre as duas matrizes obtidas determinada pelo teste Z de Mantel apresentou valor baixo, 0,31, mas significativo (p<0,001). Os resultados obtidos sugerem que os locos microssatélites aliados às informações de genealogia proporcionam melhor análise da diversidade genética de cultivares de soja.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004Coefficient of parentageGlycine max (L.) Merr.microsatellite markers
collection DOAJ
language English
format Article
sources DOAJ
author Regina Helena Geribello Priolli
José Baldin Pinheiro
Maria Imaculada Zucchi
Miklos Maximiliano Bajay
Natal Antonio Vello
spellingShingle Regina Helena Geribello Priolli
José Baldin Pinheiro
Maria Imaculada Zucchi
Miklos Maximiliano Bajay
Natal Antonio Vello
Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
Brazilian Archives of Biology and Technology
Coefficient of parentage
Glycine max (L.) Merr.
microsatellite markers
author_facet Regina Helena Geribello Priolli
José Baldin Pinheiro
Maria Imaculada Zucchi
Miklos Maximiliano Bajay
Natal Antonio Vello
author_sort Regina Helena Geribello Priolli
title Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
title_short Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
title_full Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
title_fullStr Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
title_full_unstemmed Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
title_sort genetic diversity among brazilian soybean cultivars based on ssr loci and pedigree data
publisher Instituto de Tecnologia do Paraná (Tecpar)
series Brazilian Archives of Biology and Technology
issn 1516-8913
1678-4324
publishDate 2010-06-01
description In this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD) was determined using the modified Roger's Wright distance, and a final dendrogram was in agreement with the cultivar pedigree. A distance matrix based on the coefficient of parentage scores was also generated for the cultivars, which ranged from 0 to 1, with a mean of 0.18, whereas SSR-based genetic similarity (1- GD) ranged from 0.01 to 0.90, with a mean of 0.25. Mantel's Z test showed that the similarity matrices generated from both the data sets were low, but significantly correlated (r = 0.31, p<0.001). The results showed that SSR data and pedigree analyses could help to quantify more accurately the degree of relationship among the soybean cultivars.<br>Locos microssatélites e dados de genealogia foram utilizados para avaliar a diversidade genética de um grupo de 168 cultivares brasileiras de soja. Os dezoito locos utilizados apresentaram em média 5,06 alelos por loco e coeficiente de diversidade genética médio de 0,58. O dendrograma final resultante da matriz de distância genética de Roger modificado por Wright, apresentou boa concordância com a ancestralidade dos grupos formados. Também foi estimado os coeficientes de parentesco entre as cultivares, sendo observada variação de 0 a 1 com média de 0,18, enquanto que as similaridades para os locos microssatélites (1- GD) variou de 0,01 a 0,90 com média de 0,25. A correlação entre as duas matrizes obtidas determinada pelo teste Z de Mantel apresentou valor baixo, 0,31, mas significativo (p<0,001). Os resultados obtidos sugerem que os locos microssatélites aliados às informações de genealogia proporcionam melhor análise da diversidade genética de cultivares de soja.
topic Coefficient of parentage
Glycine max (L.) Merr.
microsatellite markers
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004
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