Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
In this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD...
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Instituto de Tecnologia do Paraná (Tecpar)
2010-06-01
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doaj-1a03f6fc37184040879d2a8f68fa20852020-11-25T02:24:33ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-06-0153351953110.1590/S1516-89132010000300004Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree dataRegina Helena Geribello PriolliJosé Baldin PinheiroMaria Imaculada ZucchiMiklos Maximiliano BajayNatal Antonio VelloIn this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD) was determined using the modified Roger's Wright distance, and a final dendrogram was in agreement with the cultivar pedigree. A distance matrix based on the coefficient of parentage scores was also generated for the cultivars, which ranged from 0 to 1, with a mean of 0.18, whereas SSR-based genetic similarity (1- GD) ranged from 0.01 to 0.90, with a mean of 0.25. Mantel's Z test showed that the similarity matrices generated from both the data sets were low, but significantly correlated (r = 0.31, p<0.001). The results showed that SSR data and pedigree analyses could help to quantify more accurately the degree of relationship among the soybean cultivars.<br>Locos microssatélites e dados de genealogia foram utilizados para avaliar a diversidade genética de um grupo de 168 cultivares brasileiras de soja. Os dezoito locos utilizados apresentaram em média 5,06 alelos por loco e coeficiente de diversidade genética médio de 0,58. O dendrograma final resultante da matriz de distância genética de Roger modificado por Wright, apresentou boa concordância com a ancestralidade dos grupos formados. Também foi estimado os coeficientes de parentesco entre as cultivares, sendo observada variação de 0 a 1 com média de 0,18, enquanto que as similaridades para os locos microssatélites (1- GD) variou de 0,01 a 0,90 com média de 0,25. A correlação entre as duas matrizes obtidas determinada pelo teste Z de Mantel apresentou valor baixo, 0,31, mas significativo (p<0,001). Os resultados obtidos sugerem que os locos microssatélites aliados às informações de genealogia proporcionam melhor análise da diversidade genética de cultivares de soja.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004Coefficient of parentageGlycine max (L.) Merr.microsatellite markers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Regina Helena Geribello Priolli José Baldin Pinheiro Maria Imaculada Zucchi Miklos Maximiliano Bajay Natal Antonio Vello |
spellingShingle |
Regina Helena Geribello Priolli José Baldin Pinheiro Maria Imaculada Zucchi Miklos Maximiliano Bajay Natal Antonio Vello Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data Brazilian Archives of Biology and Technology Coefficient of parentage Glycine max (L.) Merr. microsatellite markers |
author_facet |
Regina Helena Geribello Priolli José Baldin Pinheiro Maria Imaculada Zucchi Miklos Maximiliano Bajay Natal Antonio Vello |
author_sort |
Regina Helena Geribello Priolli |
title |
Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data |
title_short |
Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data |
title_full |
Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data |
title_fullStr |
Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data |
title_full_unstemmed |
Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data |
title_sort |
genetic diversity among brazilian soybean cultivars based on ssr loci and pedigree data |
publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
series |
Brazilian Archives of Biology and Technology |
issn |
1516-8913 1678-4324 |
publishDate |
2010-06-01 |
description |
In this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD) was determined using the modified Roger's Wright distance, and a final dendrogram was in agreement with the cultivar pedigree. A distance matrix based on the coefficient of parentage scores was also generated for the cultivars, which ranged from 0 to 1, with a mean of 0.18, whereas SSR-based genetic similarity (1- GD) ranged from 0.01 to 0.90, with a mean of 0.25. Mantel's Z test showed that the similarity matrices generated from both the data sets were low, but significantly correlated (r = 0.31, p<0.001). The results showed that SSR data and pedigree analyses could help to quantify more accurately the degree of relationship among the soybean cultivars.<br>Locos microssatélites e dados de genealogia foram utilizados para avaliar a diversidade genética de um grupo de 168 cultivares brasileiras de soja. Os dezoito locos utilizados apresentaram em média 5,06 alelos por loco e coeficiente de diversidade genética médio de 0,58. O dendrograma final resultante da matriz de distância genética de Roger modificado por Wright, apresentou boa concordância com a ancestralidade dos grupos formados. Também foi estimado os coeficientes de parentesco entre as cultivares, sendo observada variação de 0 a 1 com média de 0,18, enquanto que as similaridades para os locos microssatélites (1- GD) variou de 0,01 a 0,90 com média de 0,25. A correlação entre as duas matrizes obtidas determinada pelo teste Z de Mantel apresentou valor baixo, 0,31, mas significativo (p<0,001). Os resultados obtidos sugerem que os locos microssatélites aliados às informações de genealogia proporcionam melhor análise da diversidade genética de cultivares de soja. |
topic |
Coefficient of parentage Glycine max (L.) Merr. microsatellite markers |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004 |
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