Effect of Phenolic Compounds from Elderflowers on Glucose- and Fatty Acid Uptake in Human Myotubes and HepG2-Cells
Type 2 diabetes (T2D) is manifested by progressive metabolic impairments in tissues such as skeletal muscle and liver, and these tissues become less responsive to insulin, leading to hyperglycemia. In the present study, stimulation of glucose and oleic acid uptake by elderflower extracts, constituen...
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doaj-1a04f4ed687c4f188be6446774c1e6122020-11-25T01:06:24ZengMDPI AGMolecules1420-30492017-01-012219010.3390/molecules22010090molecules22010090Effect of Phenolic Compounds from Elderflowers on Glucose- and Fatty Acid Uptake in Human Myotubes and HepG2-CellsGiang Thanh Thi Ho0Eili Tranheim Kase1Helle Wangensteen2Hilde Barsett3Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, NorwayDepartment of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, NorwayDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, NorwayDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, NorwayType 2 diabetes (T2D) is manifested by progressive metabolic impairments in tissues such as skeletal muscle and liver, and these tissues become less responsive to insulin, leading to hyperglycemia. In the present study, stimulation of glucose and oleic acid uptake by elderflower extracts, constituents and metabolites were tested in vitro using the HepG2 hepatocellular liver carcinoma cell line and human skeletal muscle cells. Among the crude extracts, the 96% EtOH extract showed the highest increase in glucose and oleic acid uptake in human skeletal muscle cells and HepG2-cells. The flavonoids and phenolic acids contained therein were potent stimulators of glucose and fatty acid uptake in a dose-dependent manner. Most of the phenolic constituents and several of the metabolites showed high antioxidant activity and showed considerably higher α-amylase and α-glucosidase inhibition than acarbose. Elderflower might therefore be valuable as a functional food against diabetes.http://www.mdpi.com/1420-3049/22/1/90elderflowerSambucus nigratype 2 diabeteshuman skeletal muscleHepG2-cellmetabolic disorderantioxidantglucose uptakeoleic acid uptake |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giang Thanh Thi Ho Eili Tranheim Kase Helle Wangensteen Hilde Barsett |
spellingShingle |
Giang Thanh Thi Ho Eili Tranheim Kase Helle Wangensteen Hilde Barsett Effect of Phenolic Compounds from Elderflowers on Glucose- and Fatty Acid Uptake in Human Myotubes and HepG2-Cells Molecules elderflower Sambucus nigra type 2 diabetes human skeletal muscle HepG2-cell metabolic disorder antioxidant glucose uptake oleic acid uptake |
author_facet |
Giang Thanh Thi Ho Eili Tranheim Kase Helle Wangensteen Hilde Barsett |
author_sort |
Giang Thanh Thi Ho |
title |
Effect of Phenolic Compounds from Elderflowers on Glucose- and Fatty Acid Uptake in Human Myotubes and HepG2-Cells |
title_short |
Effect of Phenolic Compounds from Elderflowers on Glucose- and Fatty Acid Uptake in Human Myotubes and HepG2-Cells |
title_full |
Effect of Phenolic Compounds from Elderflowers on Glucose- and Fatty Acid Uptake in Human Myotubes and HepG2-Cells |
title_fullStr |
Effect of Phenolic Compounds from Elderflowers on Glucose- and Fatty Acid Uptake in Human Myotubes and HepG2-Cells |
title_full_unstemmed |
Effect of Phenolic Compounds from Elderflowers on Glucose- and Fatty Acid Uptake in Human Myotubes and HepG2-Cells |
title_sort |
effect of phenolic compounds from elderflowers on glucose- and fatty acid uptake in human myotubes and hepg2-cells |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2017-01-01 |
description |
Type 2 diabetes (T2D) is manifested by progressive metabolic impairments in tissues such as skeletal muscle and liver, and these tissues become less responsive to insulin, leading to hyperglycemia. In the present study, stimulation of glucose and oleic acid uptake by elderflower extracts, constituents and metabolites were tested in vitro using the HepG2 hepatocellular liver carcinoma cell line and human skeletal muscle cells. Among the crude extracts, the 96% EtOH extract showed the highest increase in glucose and oleic acid uptake in human skeletal muscle cells and HepG2-cells. The flavonoids and phenolic acids contained therein were potent stimulators of glucose and fatty acid uptake in a dose-dependent manner. Most of the phenolic constituents and several of the metabolites showed high antioxidant activity and showed considerably higher α-amylase and α-glucosidase inhibition than acarbose. Elderflower might therefore be valuable as a functional food against diabetes. |
topic |
elderflower Sambucus nigra type 2 diabetes human skeletal muscle HepG2-cell metabolic disorder antioxidant glucose uptake oleic acid uptake |
url |
http://www.mdpi.com/1420-3049/22/1/90 |
work_keys_str_mv |
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