Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes
While antibiotic-resistant bacteria have been detected in extreme environments, including Antarctica, to date there are no reports of Acinetobacter species isolated from this region. Here, we characterized by whole-genome sequencing (WGS) the genetic content of a single antibiotic-resistant Acinetob...
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Format: | Article |
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Frontiers Media S.A.
2019-09-01
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Series: | Frontiers in Cellular and Infection Microbiology |
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Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2019.00328/full |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrés Opazo-Capurro Andrés Opazo-Capurro Paul G. Higgins Paul G. Higgins Julia Wille Julia Wille Harald Seifert Harald Seifert Camila Cigarroa Paulina González-Muñoz Paulina González-Muñoz Paulina González-Muñoz Mario Quezada-Aguiluz Mario Quezada-Aguiluz Mariana Domínguez-Yévenes Mariana Domínguez-Yévenes Helia Bello-Toledo Helia Bello-Toledo Luis Vergara Gerardo González-Rocha Gerardo González-Rocha |
spellingShingle |
Andrés Opazo-Capurro Andrés Opazo-Capurro Paul G. Higgins Paul G. Higgins Julia Wille Julia Wille Harald Seifert Harald Seifert Camila Cigarroa Paulina González-Muñoz Paulina González-Muñoz Paulina González-Muñoz Mario Quezada-Aguiluz Mario Quezada-Aguiluz Mariana Domínguez-Yévenes Mariana Domínguez-Yévenes Helia Bello-Toledo Helia Bello-Toledo Luis Vergara Gerardo González-Rocha Gerardo González-Rocha Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes Frontiers in Cellular and Infection Microbiology antibiotic-resistance genes Acinetobacter Antarctica whole-genome sequencing resistance plasmid ecotoxicology |
author_facet |
Andrés Opazo-Capurro Andrés Opazo-Capurro Paul G. Higgins Paul G. Higgins Julia Wille Julia Wille Harald Seifert Harald Seifert Camila Cigarroa Paulina González-Muñoz Paulina González-Muñoz Paulina González-Muñoz Mario Quezada-Aguiluz Mario Quezada-Aguiluz Mariana Domínguez-Yévenes Mariana Domínguez-Yévenes Helia Bello-Toledo Helia Bello-Toledo Luis Vergara Gerardo González-Rocha Gerardo González-Rocha |
author_sort |
Andrés Opazo-Capurro |
title |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_short |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_full |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_fullStr |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_full_unstemmed |
Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes |
title_sort |
genetic features of antarctic acinetobacter radioresistens strain a154 harboring multiple antibiotic-resistance genes |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2019-09-01 |
description |
While antibiotic-resistant bacteria have been detected in extreme environments, including Antarctica, to date there are no reports of Acinetobacter species isolated from this region. Here, we characterized by whole-genome sequencing (WGS) the genetic content of a single antibiotic-resistant Acinetobacter spp. isolate (A154) collected in Antarctica. The isolate was recovered in 2013 from soil samples at Fildes Peninsula, Antarctica, and was identified by detection of the intrinsic OXA-23 gene, and confirmed by Tetra Correlation Search (TCS) and WGS. The antibiotic susceptibility profile was determined by disc diffusion, E-test, and broth microdilution methods. From WGS data, the acquired resistome and insertion sequence (IS) content were identified by in silico analyses. Plasmids were studied by the alkaline lysis method followed by pulsed-field gel electrophoresis and conventional PCR. The A154 isolate was identified as A. radioresistens by WGS analysis and displayed >99.9 of similarity by TCS in relation with the databases. Moreover, it was resistant to ampicillin, ceftriaxone, ceftazidime, cefepime, cefotaxime, streptomycin, and kanamycin. Likewise, in addition to the intrinsic blaOXA−23−like gene, A154 harbored the plasmid-encoded antibiotic-resistance genes blaPER−2, tet(B), aph(3′)-Vla, strA, and strB, as well as a large diversity of ISs. This is the first report of antibiotic-resistant A. radioresistens in Antarctica. Our findings show the presence of several resistance genes which could be either intrinsic or acquired in the region. |
topic |
antibiotic-resistance genes Acinetobacter Antarctica whole-genome sequencing resistance plasmid ecotoxicology |
url |
https://www.frontiersin.org/article/10.3389/fcimb.2019.00328/full |
work_keys_str_mv |
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doaj-1a1754041de74520915d334bb9714f202020-11-24T21:27:23ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-09-01910.3389/fcimb.2019.00328463286Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance GenesAndrés Opazo-Capurro0Andrés Opazo-Capurro1Paul G. Higgins2Paul G. Higgins3Julia Wille4Julia Wille5Harald Seifert6Harald Seifert7Camila Cigarroa8Paulina González-Muñoz9Paulina González-Muñoz10Paulina González-Muñoz11Mario Quezada-Aguiluz12Mario Quezada-Aguiluz13Mariana Domínguez-Yévenes14Mariana Domínguez-Yévenes15Helia Bello-Toledo16Helia Bello-Toledo17Luis Vergara18Gerardo González-Rocha19Gerardo González-Rocha20Laboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileMillennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago, ChileInstitute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, GermanyGerman Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, GermanyGerman Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, GermanyGerman Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, GermanyLaboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileMillennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago, ChileDepartamento de Ciencias Biológicas y Químicas, Facultad de Medicina y Ciencia, Universidad San Sebastián, Concepción, ChileLaboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileMillennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago, ChileLaboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileMillennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago, ChileLaboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileMillennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago, ChileDepartamento de Ciencias Biológicas y Químicas, Facultad de Medicina y Ciencia, Universidad San Sebastián, Concepción, ChileLaboratorio de Investigación en Agentes Antibacterianos (LIAA), Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileMillennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago, ChileWhile antibiotic-resistant bacteria have been detected in extreme environments, including Antarctica, to date there are no reports of Acinetobacter species isolated from this region. Here, we characterized by whole-genome sequencing (WGS) the genetic content of a single antibiotic-resistant Acinetobacter spp. isolate (A154) collected in Antarctica. The isolate was recovered in 2013 from soil samples at Fildes Peninsula, Antarctica, and was identified by detection of the intrinsic OXA-23 gene, and confirmed by Tetra Correlation Search (TCS) and WGS. The antibiotic susceptibility profile was determined by disc diffusion, E-test, and broth microdilution methods. From WGS data, the acquired resistome and insertion sequence (IS) content were identified by in silico analyses. Plasmids were studied by the alkaline lysis method followed by pulsed-field gel electrophoresis and conventional PCR. The A154 isolate was identified as A. radioresistens by WGS analysis and displayed >99.9 of similarity by TCS in relation with the databases. Moreover, it was resistant to ampicillin, ceftriaxone, ceftazidime, cefepime, cefotaxime, streptomycin, and kanamycin. Likewise, in addition to the intrinsic blaOXA−23−like gene, A154 harbored the plasmid-encoded antibiotic-resistance genes blaPER−2, tet(B), aph(3′)-Vla, strA, and strB, as well as a large diversity of ISs. This is the first report of antibiotic-resistant A. radioresistens in Antarctica. Our findings show the presence of several resistance genes which could be either intrinsic or acquired in the region.https://www.frontiersin.org/article/10.3389/fcimb.2019.00328/fullantibiotic-resistance genesAcinetobacterAntarcticawhole-genome sequencingresistance plasmidecotoxicology |