Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in Mice
Radix angelicae pubescentis (RAP) has been used in Chinese traditional medicine to treat painful diseases such as rheumatism and headache. A previous study has reported that columbianadin (CBN), a major coumarin in RAP inhibits acute and inflammatory pain behaviors. However, the effects of CBN on ne...
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doaj-1a17dbce87704fdfa245a24195bca2692020-11-25T01:30:01ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-01-011010.3389/fphar.2019.01522456065Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in MiceXiaolin Su0Xiaolin Su1Bin Wu2Bin Wu3Wentong Zhang4Yong-Hua Ji5Qiuhong Wang6Qiuhong Wang7Zhi-Yong Tan8Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Harbin, ChinaDepartment of Pharmacology and Toxicology and Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Pharmacology and Toxicology and Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, United StatesInstitute of Special Environment Medicine, Nantong University, Nantong, ChinaDepartment of Pharmacology and Toxicology and Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, United StatesLab of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai, ChinaKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Harbin, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, ChinaDepartment of Pharmacology and Toxicology and Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, United StatesRadix angelicae pubescentis (RAP) has been used in Chinese traditional medicine to treat painful diseases such as rheumatism and headache. A previous study has reported that columbianadin (CBN), a major coumarin in RAP inhibits acute and inflammatory pain behaviors. However, the effects of CBN on neuropathic pain behaviors, and the potential underlying mechanism have not been reported. In the present study, the effects of CBN, compared to another major coumarin of RAP osthole (OST), on oxaliplatin-induced neuropathic pain behaviors and on the voltage-gated calcium currents in small dorsal root ganglion (DRG) neurons were studied in mice. It was found that CBN and OST inhibited both mechanical and cold hypersensitivity induced by oxaliplatin. Moreover, CBN and OST might preferentially inhibit T- and L-type calcium currents (Ica). The inhibitory effects of CBN and OST on the oxaliplatin-induced mechanical allodynia were prevented by gabapentin. These results suggest that CBN, as well as OST might inhibit neuropathic pain behaviors through an inhibition of T- and L-type calcium currents in nociceptive DRG neurons.https://www.frontiersin.org/article/10.3389/fphar.2019.01522/fullcolumbianadinRadix angelicae pubescentistraditional Chinese medicineneuropathic paincalcium currentsoxaliplatin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaolin Su Xiaolin Su Bin Wu Bin Wu Wentong Zhang Yong-Hua Ji Qiuhong Wang Qiuhong Wang Zhi-Yong Tan |
spellingShingle |
Xiaolin Su Xiaolin Su Bin Wu Bin Wu Wentong Zhang Yong-Hua Ji Qiuhong Wang Qiuhong Wang Zhi-Yong Tan Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in Mice Frontiers in Pharmacology columbianadin Radix angelicae pubescentis traditional Chinese medicine neuropathic pain calcium currents oxaliplatin |
author_facet |
Xiaolin Su Xiaolin Su Bin Wu Bin Wu Wentong Zhang Yong-Hua Ji Qiuhong Wang Qiuhong Wang Zhi-Yong Tan |
author_sort |
Xiaolin Su |
title |
Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in Mice |
title_short |
Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in Mice |
title_full |
Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in Mice |
title_fullStr |
Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in Mice |
title_full_unstemmed |
Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in Mice |
title_sort |
inhibitory effects of columbianadin on nociceptive behaviors in a neuropathic pain model, and on voltage-gated calcium currents in dorsal root ganglion neurons in mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2020-01-01 |
description |
Radix angelicae pubescentis (RAP) has been used in Chinese traditional medicine to treat painful diseases such as rheumatism and headache. A previous study has reported that columbianadin (CBN), a major coumarin in RAP inhibits acute and inflammatory pain behaviors. However, the effects of CBN on neuropathic pain behaviors, and the potential underlying mechanism have not been reported. In the present study, the effects of CBN, compared to another major coumarin of RAP osthole (OST), on oxaliplatin-induced neuropathic pain behaviors and on the voltage-gated calcium currents in small dorsal root ganglion (DRG) neurons were studied in mice. It was found that CBN and OST inhibited both mechanical and cold hypersensitivity induced by oxaliplatin. Moreover, CBN and OST might preferentially inhibit T- and L-type calcium currents (Ica). The inhibitory effects of CBN and OST on the oxaliplatin-induced mechanical allodynia were prevented by gabapentin. These results suggest that CBN, as well as OST might inhibit neuropathic pain behaviors through an inhibition of T- and L-type calcium currents in nociceptive DRG neurons. |
topic |
columbianadin Radix angelicae pubescentis traditional Chinese medicine neuropathic pain calcium currents oxaliplatin |
url |
https://www.frontiersin.org/article/10.3389/fphar.2019.01522/full |
work_keys_str_mv |
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