Tctex-1 augments G protein-coupled receptor-mediated Gs signaling by activating adenylyl cyclase

• Main Authors: Masaki Saito, Ayano Chiba, Takeya Sato, Takahiro Moriya, Jun Sukegawa, Norimichi Nakahata
• Format: Article
• Language: English
• Published: Elsevier 2021-01-01
• Series: Journal of Pharmacological Sciences
• Subjects: Cytoplasmic dynein light chain Tctex-1; Parathyroid hormone receptor; Adenylyl cyclase
• Online Access: http://www.sciencedirect.com/science/article/pii/S1347861320301171
• ISSN: 1347-8613

Proteins interacting with G protein-coupled receptors (GPCRs) can modulate signal transduction of these receptors. However, the regulatory mechanisms of the interacting proteins are diverse and largely unknown. We have previously shown that Tctex-1 (or DYNLT1) can interact with the parathyroid hormone receptor (PTHR). In the present study, we investigated the role of Tctex-1 in the PTHR signaling and found that Tctex-1 augmented the PTHR-mediated Gs/adenylyl cyclase (AC) pathway by activating AC regardless of the binding to PTHR. Furthermore, Tctex-1 directly bound to AC type 6. These data demonstrate a novel mechanism underlying GPCR/Gs signaling regulated by Tctex-1.