Propolis Extract: A Possible Antiseptic Oral Care against Multidrug-Resistant Non-Fermenting Bacteria Isolated from Non-Ventilator Hospital-Acquired Pneumonia
Non-ventilator Hospital-acquired Pneumonia (NV-HAP) is a significant burden in acute care hospitals and poses a risk to nonelderly, non-intensive care unit (ICU) patients, which have been increasing worldwide. In addition, poor oral hygiene has been associated to significant increases in the numbe...
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doaj-1a32b5704ade47f58a74132b26fffa102021-10-02T18:52:31ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2020-03-0114112313110.22207/JPAM.14.1.13Propolis Extract: A Possible Antiseptic Oral Care against Multidrug-Resistant Non-Fermenting Bacteria Isolated from Non-Ventilator Hospital-Acquired PneumoniaSylvana N. Gaber0https://orcid.org/0000-0001-5757-867XEman Elsayed Mahmoud Hemeda1Hebat-Allah Sayed Elsayeh2Wafaa Y. Abdel Wahed3https://orcid.org/0000-0002-8524-6135Mahmoud A.F. Khalil4Enas G. Ibrahim5Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Fayoum, Egypt. Department of Clinical and Chemical Pathology, Faculty of Medicine, Fayoum University, Fayoum, Egypt. Departments of Apiculture Research, Plant Protectionsititue, A.R.C.Department of Public Health, Faculty of Medicine, Fayoum University, Fayoum, Egypt.Department of Medical Microbiology and Immunology, Faculty of Pharmacy, Fayoum University, Fayoum, Egypt.Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Fayoum, Egypt. Non-ventilator Hospital-acquired Pneumonia (NV-HAP) is a significant burden in acute care hospitals and poses a risk to nonelderly, non-intensive care unit (ICU) patients, which have been increasing worldwide. In addition, poor oral hygiene has been associated to significant increases in the number of cases of NV-HAP. Unfortunately, preventive options are limited. Thus, there is a need for oral antiseptics, similar to those of natural products or plant sources. The aim of this study was to assess the antibacterial activity of various bee products (BPs); for example, honey, propolis, and bee venom against multidrugresistant (MDR) non-fermenting bacteria (e.g., Pseudomonas and Acinetobacter), which were collected from NV-HAP patients to investigate its use as a possible antiseptic oral care. Bacterial susceptibility to different antibiotics were performed. The antimicrobial activity of BPs against non-fermenting bacteria, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) were assessed. Eighteen Pseudomonas aeruginosa isolates and five Acinetobacter baumannii isolates were identified. P. aeruginosa isolates displayed high resistance to the antibiotics: meropenem and imipenem (55.6% and 77.8% respectively), whereas A. baumannii isolates were 100% resistant to meropenem and imipenem. All isolates remained sensitive to colistin. Propolis showed the best antibacterial activity (p<0.001) in comparison to honey and bee venom against P. aeruginosa (13 - 36 mm, MIC =1.4-22.5%, and MBC=2.8-45%) and A. baumannii (7-20 mm, MIC=5.6-22.5%, and MBC=11.3 -22.5%). While bee venom expressed the least antibacterial activity against all isolates with a zone diameter ranging from 0-12 mm, propolis, which is a non-toxic, natural, and inexpensive, had antibacterial activity towards the MDR bacteria: P. aeruginosa and A. baumannii collected from pneumonic patients. Additionally, we confirmed that propolis could be used as a potential antiseptic oral care product.https://microbiologyjournal.org/propolis-extract-a-possible-antiseptic-oral-care-against-multidrug-resistant-non-fermenting-bacteria-isolated-from-non-ventilator-hospital-acquired-pneumonia/antimicrobial activitypropolis extractpneumoniapseudomonas aeruginosa |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sylvana N. Gaber Eman Elsayed Mahmoud Hemeda Hebat-Allah Sayed Elsayeh Wafaa Y. Abdel Wahed Mahmoud A.F. Khalil Enas G. Ibrahim |
spellingShingle |
Sylvana N. Gaber Eman Elsayed Mahmoud Hemeda Hebat-Allah Sayed Elsayeh Wafaa Y. Abdel Wahed Mahmoud A.F. Khalil Enas G. Ibrahim Propolis Extract: A Possible Antiseptic Oral Care against Multidrug-Resistant Non-Fermenting Bacteria Isolated from Non-Ventilator Hospital-Acquired Pneumonia Journal of Pure and Applied Microbiology antimicrobial activity propolis extract pneumonia pseudomonas aeruginosa |
author_facet |
Sylvana N. Gaber Eman Elsayed Mahmoud Hemeda Hebat-Allah Sayed Elsayeh Wafaa Y. Abdel Wahed Mahmoud A.F. Khalil Enas G. Ibrahim |
author_sort |
Sylvana N. Gaber |
title |
Propolis Extract: A Possible Antiseptic Oral Care against Multidrug-Resistant Non-Fermenting Bacteria Isolated from Non-Ventilator Hospital-Acquired Pneumonia |
title_short |
Propolis Extract: A Possible Antiseptic Oral Care against Multidrug-Resistant Non-Fermenting Bacteria Isolated from Non-Ventilator Hospital-Acquired Pneumonia |
title_full |
Propolis Extract: A Possible Antiseptic Oral Care against Multidrug-Resistant Non-Fermenting Bacteria Isolated from Non-Ventilator Hospital-Acquired Pneumonia |
title_fullStr |
Propolis Extract: A Possible Antiseptic Oral Care against Multidrug-Resistant Non-Fermenting Bacteria Isolated from Non-Ventilator Hospital-Acquired Pneumonia |
title_full_unstemmed |
Propolis Extract: A Possible Antiseptic Oral Care against Multidrug-Resistant Non-Fermenting Bacteria Isolated from Non-Ventilator Hospital-Acquired Pneumonia |
title_sort |
propolis extract: a possible antiseptic oral care against multidrug-resistant non-fermenting bacteria isolated from non-ventilator hospital-acquired pneumonia |
publisher |
Journal of Pure and Applied Microbiology |
series |
Journal of Pure and Applied Microbiology |
issn |
0973-7510 2581-690X |
publishDate |
2020-03-01 |
description |
Non-ventilator Hospital-acquired Pneumonia (NV-HAP) is a significant burden in acute care hospitals and
poses a risk to nonelderly, non-intensive care unit (ICU) patients, which have been increasing worldwide.
In addition, poor oral hygiene has been associated to significant increases in the number of cases of
NV-HAP. Unfortunately, preventive options are limited. Thus, there is a need for oral antiseptics, similar
to those of natural products or plant sources. The aim of this study was to assess the antibacterial
activity of various bee products (BPs); for example, honey, propolis, and bee venom against multidrugresistant (MDR) non-fermenting bacteria (e.g., Pseudomonas and Acinetobacter), which were collected
from NV-HAP patients to investigate its use as a possible antiseptic oral care. Bacterial susceptibility to
different antibiotics were performed. The antimicrobial activity of BPs against non-fermenting bacteria,
the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) were
assessed. Eighteen Pseudomonas aeruginosa isolates and five Acinetobacter baumannii isolates were
identified. P. aeruginosa isolates displayed high resistance to the antibiotics: meropenem and imipenem
(55.6% and 77.8% respectively), whereas A. baumannii isolates were 100% resistant to meropenem and
imipenem. All isolates remained sensitive to colistin. Propolis showed the best antibacterial activity
(p<0.001) in comparison to honey and bee venom against P. aeruginosa (13 - 36 mm, MIC =1.4-22.5%,
and MBC=2.8-45%) and A. baumannii (7-20 mm, MIC=5.6-22.5%, and MBC=11.3 -22.5%). While bee
venom expressed the least antibacterial activity against all isolates with a zone diameter ranging from
0-12 mm, propolis, which is a non-toxic, natural, and inexpensive, had antibacterial activity towards
the MDR bacteria: P. aeruginosa and A. baumannii collected from pneumonic patients. Additionally,
we confirmed that propolis could be used as a potential antiseptic oral care product. |
topic |
antimicrobial activity propolis extract pneumonia pseudomonas aeruginosa |
url |
https://microbiologyjournal.org/propolis-extract-a-possible-antiseptic-oral-care-against-multidrug-resistant-non-fermenting-bacteria-isolated-from-non-ventilator-hospital-acquired-pneumonia/ |
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